Adem GÜNEŞ, Metin TURAN, Medine GÜLLÜCE, Fikrettin ŞAHİN, Mehmet Rüştü KARAMAN

Farklı bakteri Uygulamalarının Kaya Fosfatının Çözünürlüğü Üzerine Etkisi

Bu çalışmada, iki farklı kaynaktan sağlanan fosfat kayasının çözünürlüğü üzerine Azospirillum brasilense SP-245, Bacillus subtilis OSU-142, Bacillus megaterium M3, Raoultella terrigena, Burkholderia cepacia BA-7 fosfor çözücü bakterilerin çözelti ortamındaki etkisi araştırılmıştır. Laboratuvar koşullarında yürütülen denemede uygulamaları takiben deneme süresince 3 örnekleme (10., 20. ve 30. gün) yapılarak, elektriksel iletkenlik (EC) ve pH değerleri ile suda çözünebilir kalsiyum ve fosfor miktarları ölçülmüştür. Bakteri uygulamaları her iki fosforfor kaynağının kullanıldığı çözelti pH'sında azalışa neden olurken, elektriksel iletkenlik, fosfor ve Ca içeriğinde artışa neden olmuştur. En düşük pH değeri BA-7 uygulamasından elde edilirken, en yüksek P, Ca, çözünebilir tuz konsantrasyonu en yüksek asit ve alkalin fosfataz enzim aktivitesi ve organik asit salgılama kapasitesine sahip B. megaterium M3 uygulamasının 30. gün inkübasyonundan elde edilmiştir.

Anahtar Kelimeler:

Enzim aktivitesi, Fosfat kayası, fosfor çözünürlüğü, Enzyme activity, Phosphate rock, phosphorus solubilization.

Effects of Different Bacteria Application on Solubility of Rock Phosphate

In the present study, the capacity of phosphate solubilizing bacterial strain, Azospirillum brasilense SP-245, Bacillus subtilis OSU-142, Bacillus megaterium M3, Raoultella terrigena, Burkholderia cepacia BA-7 have been tested solution culture containing two different rock phosphate sources. Experiment was carried out under laboratory conditions during experiment period 3 times sample was collected (10th, 20th, and 30th day). The phosphorus solubilizing bacteria inoculation decreased solution pH and increased electrical conductivity, and Ca and P concentrations in solution culture of both P source. While the largest pH decrease was found with B. cepacia BA-7, the highest EC values, Ca and P concentrations were found applications of B. megaterium M3 which has the highest acid -alkaline phosphatase enzyme activities and organic acid compound when compared to control during the 30th day of the experiment.

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