Türkiye’den İzole Edilen Entomopatojen Nematodlar, Steinernema weiseri ve S. feltiae Rhabditida: Steinernematidae ’nin Bazı Biyolojik Özelliklerinin Karşılaştırılması

Türkiye’de ilk kez entomopatojen nematod Steinernema weiseri Ankara, Hacettepe Üniversitesi Beytepe Kampüsünden ve S. feltiae Rhabditida: Steinernematidae Ankara Üniversitesi kampüsünden izole edilmiştir. Bu çalışmada her iki entomopatojen nematodun değişik koşullarda etkinlik ve üreme kapasiteleri karşılaştırılmıştır. Üreme kapasiteleri Balmumu Güvesi, Galleria mellonella L. Lep: Pyralidae ‘nın son dönem larvaları üzerine 10, 15 ve 20 ºC sıcaklıklarda 10, 50 ve 100 adet infektif larva nematodun uygulanması ile belirlenmiştir. Konukçu larva içerisinde üreyerek kadavradan dışarı çıkan her iki nematoda ait yeni generasyon jüvenilleri sayılmıştır. Tüm deneme koşullarında S. weiserii’nin S. feltiae’ye göre daha fazla üreme kapasitesine sahip olduğu belirlenmiştir. G. mellonella’nın son dönem larvaları üzerinde her iki nematodun etkinliği penetrasyondan 48 saat sonra LD50 olarak belirlenmiştir. Bu sonuçlara göre S. weiseri’nin, S. feltiae’ye göre daha etkili olduğu ve biyolojik mücadelede kullanımının daha yararlı olabileceği bulunmuştur

Anahtar Kelimeler:

Etkinlik, üreme kapasitesi, entomopatojen nematodlar, Steinernema weiseri, S. feltiae, Türkiye

Comparison of Some Biological Characterizations of the Entomopathogenic Nematodes, Steinernema weiseri and S. feltiae Rhabditida: Steinernematidae , Isolated in Turkey

The entomopathogenic nematodes, Steinernema weiseri, was firstly isolated in Beytepe Campus of Hacettepe University, in Ankara, Turkey and S. feltiae Rhabditida: Steinernematidae , which was found in Ankara University Campus. In the present study, reproduction capacities and effectiveness of the both entomopathogenic nematodes were compared in variable conditions. The reproduction capacities were examined at the following temperatures; 10, 15 and 20 ºC and the doses of 10, 50 and 100 infective juvenilesdauer juveniles DJs in the last instar larvae of greater wax moth, Galleria mellonella L. Lep: Pyralidae . New generation DJs of each species, which produce within the host larvae and emerging from cadaver were counted and the results showed that S. weiseri was more productive than S. feltiae in all experimental conditions. Effectiveness of the both species on the last instar G. mellonella larvae as LD50 was calculated 48 h after penetration. According to the results, S. weiseri is more effective than S. feltiae. The present results indicated that S. weiseri can be more useful for biological control

Keywords:

Effectiveness, reproduction capacity, entomopathogenic nematodes, Steinernema weiseri, S,

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