Umut TOPRAK, İ. Alper SUSURLUK, M. Oktay GÜRKAN

Türk SpliNPV-B İzolatından elde edilen lef-8 Geninin diğer Bakulovirüslerle Kısmi Sekans Karşılaştırması

Spodoptera littoralis Boisd. Lepidoptera:Noctuidae nukleopolihedrovirus SpliNPV Baculoviridae’nin bir üyesi olup grup II NPV’leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV’sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B SpliNPV-TR1 genomundaki lef-8 geni PCR’la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir en fazla % 46 . Grup II NPV’leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV’leri arasında saptanan düşük dereceli benzerlikler SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç , bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir

Anahtar Kelimeler:

Bakulovirüs, Spodoptera littoralis, Noctuidae, SpliNPV-TR1, lef-8, sekans, karşılaştırma

The Partial Sequence Comparison of lef-8 gene Obtained from a Turkish SpliNPV–B with Several Baculoviruses

Spodoptera littoralis Boisd. Lepidoptera:Noctuidae nucleopolyhedrovirus SpliNPV is a member of Baculoviridae and belongs to the group II NPVs. Lef-8 is a well-conserved gene that is characterized in most of the lepidopteran NPVs. Nucleotide and aminoacid sequences of the encoded proteins and gene content may be phylogenetically informative. In this study, lef-8 gene in the Turkish SpliNPV-B SpliNPV-TR1 genome was partially amplified by PCR and analyzed by amino-acid sequences with several baculoviruses. Amino-acid sequences of partial SpliNPV-TR1 lef-8 gene was 84 % identical to the lef-8 of SpltNPV while revealed it to have about only 57 %, 55 %, 51 %, 50 %, 50 %, 50 % and 49 % of its amino-acid sequence identity with AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV and BufuNPV, respectively. The sequences between SpliNPV-TR1 and granuloviruses also produced low levels of sequence identities maximum 46 % . Although group II NPVs were rather isolated from noctuid hosts, the low level identity values detected between these noctuid NPVs except the high idendity value between SpliNPV-TR1 and SpltNPV revealed that these isolates can not have a monophyletic origin

Keywords:

Baculovirus, spodoptera littoralis, noctuidae, spliNPV-TR1, lef-8, sequence, comparison,

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