‘Tombul’ Fındık Çeşidinde Olgunlaşmamış Kotiledonlardan Somatik Embriyo Oluşumu

Açıkta tozlanan „Tombul‟ fındık çeşidinde, 6-benziladenin‟ in BA , 2,4- diklorofenoksiasetik asit 2,4-D ve Naftalenasetik asit NAA ile kombinasyonlarının ve kotiledon olgunlaşma düzeyinin somatik embriyo oluşumu üzerine etkileri incelenmiştir. Olgunlaşmamış kotiledon eksplantları hasattan 45 gün 1 Temmuz ve 30 gün 15 Temmuz önce alınmıştır. En yüksek somatik embriyo oluşumu %66.7 ve eksplant başına en yüksek somatik embriyo sayısı 5.5 1.0 mg·L-1BA, 0.5 mg·L-1 NAA ve 250 mg·L-1 L-glutamin ilave edilmiş ½ makro element içeren Murashige ve Skoog MS temel besin ortamından elde edilmiştir. Somatik embriyo oluşumu üzerine hasattan 45 gün önce alınan kotiledonların 30 gün önce alınanlara göre daha etkili olduğu belirlenmiştir

Anahtar Kelimeler:

Corylus avellana, somatik embriyo, kotiledon olgunluğu, 6-benziladenin, 2, 4diklorofenoksi asetik asit, α-naftalenasetik asit

Induction of Somatic Embryogenesis from Immature Cotyledons in ‘Tombul’ Hazelnut

Effects of 6-benzyladenine BA in combination with auxins either 2,4-dichlorophenoxyacetic acid 2,4-D or α-naphthaleneacetic acid NAA on the induction of somatic embryogenesis in different cotyledon maturity stages of open-pollinated seeds of „Tombul‟ hazelnut cultivar were investigated. Immature cotyledon explants were collected 45 and 30 days before harvest on July 1 and July 15 respectively. Initial Murashige and Skoog MS basal medium with half-strength of macronutrients containing 1.0 mg L-1 BA, 0.5 mg L-1 NAA and 250 mg L-1 L-glutamine resulted in the highest embryogenesis 66.7% and the highest number of somatic embryo 5.5 per cotyledon explant. The cotyledons collected 45 days before the harvest were more inductive than those collected 30 days before the harvest on embryogenesis. Key dichlorophenoxyacetic acid, α-naphthaleneacetic acid Words: Corylus avellana, somatic embryo, cotyledon maturity, 6-benzyladenine, 2,4

Keywords:

Corylus avellana, somatic embryo, cotyledon maturity, 6-benzyladenine, 2, 4- dichlorophenoxyacetic acid, α-naphthaleneacetic acid,

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