Taze Çay Yaprağı ve Yeşil Çayın Ekstrakt ve Fraksiyonlarının Antioksidan ve Antibakteriyel Etkileri

Taze çay yaprağı FTL ve yeşil çayın GT metanol, etanol ve su ham ekstraktları ile bu ekstraktların etil asetat ve su fraksiyonlarının polifenol içeriği, polifenol verimi, antioksidan ve antimikrobiyel aktiviteleri belirlenmiştir. FTL ve GT örneklerinde etanol ekstraktlarının etil asetat fraksiyonunun en yüksek düzeyde polifenol içerdiği sırasıyla 680.2 ve 560.8 mg gallik asit eşdeğeri g-1 belirlenmiştir. Her üç ekstraksiyon solventinde de etil asetat fraksiyonu en yüksek polifenol içeriğine sahiptir. En yüksek antioksidatif aktiviteyi FTL ve GT’nın su ekstraktlarının etil asetat fraksiyonu göstermiştir sırasıyla 80.8 ve 78.6 g askorbik asit eşdeğeri 100 g-1 kuru ekstrakt . FTL ve GT örneklerinin antioksidan aktivitesi ile toplam fenolik madde miktarı arasında oldukça yüksek sırasıyla R2 = 0.9376 ve 0.9783 bir korelasyon mevcuttur. Her iki çay örneğinin etil asetat fraksiyonu S. aureus ve B. cereus üzerinde antibakteriyel etki gösterirken FTL’nin ham ekstraktı S. aureus üzerinde etki göstermiştir

Antioxidant and Antibacterial Activities of Various Extracts and Fractions of Fresh Tea Leaves and Green Tea

Polyphenol content, polyphenol yield, antioxidant and antibacterial activities of methanol, ethanol and water extracts and their crude, ethyl acetate and water fractions derived from fresh tea leaves FTL and green tea GT were evaluated. Ethyl acetate fractions of ethanolic extracts contained the highest polyphenol content in FTL and GT samples 680.2 and 560.8 mg gallic acid equivalent g-1 dry extract, respectively . For each of 3 extraction solvents, ethyl acetate fractions always had highest polyphenol content, for both teas. Ethyl acetate fractions of water extracts of FTL and GT had highest antioxidant activity 80.8 and 78.6 g ascorbic acid 100g-1 dry extract . A rather high correlation coefficient R2 = 0.9376 for FTL and 0.9783 for GT was obtained between antioxidant activity to total polyphenol content for both tea. Ethyl acetate fractions of extracts showed antibacterial activity on S. aureus and B. cereus. Crude extract of FTL were also effective on S. aureus

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  • Alzoreky, N.S. and K. Nakahara. 2003. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology 80: 223-230.
  • An, B., J. Kwak, J. Son, J. Park, J.C. Lee and M. Byun. 2004. Biological and anti-microbial activity of irradiated green tea polyphenols. Food Chemistry 88: 549-555.
  • Bu-Abbas, A., E. Copeland, M. N. Clifford, R. Walker and C. Ioannides. 1997.Fractionation of green tea extracts: correlation of antimutagenic effect with flavanol content. Journal of the Science of Food and Agriculture 75: 453- 462.
  • Canadanovic-Brunet, J.M., S.M. Djilas and G.S. Cetkovic. 2005. Free-radical scavenging activity of wormwood (Artemisia absinthium) extracts. Journal of the Science of Food and Agriculture 85: 265-272.
  • Chan, E.W.C., Y.Y. Lim and Y.L. Chew. 2007. Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food Chemistry 102: 1214-1222.
  • Chen, Z., S. Wang, K.M.S. Lee, Y. Huang and W.K.K. Ho. 2001. Preparation of flavanol-rich green tea extract by precipitation with AlCl3. Journal of the Science of Food and Agriculture 81: 1034-1038.
  • Chou, C., L. Lin and K. Chung. 1999. Antimicrobial activity of tea as affected by the degree of fermentation and manufacturing season. International Journal of Food Microbiology 48: 125-130.
  • Clifford, M.N., E.L. Copeland, J.P. Bloxsidge and L.A. Mitchell. 2000. Hippuric acid as a major excretion product associated with black tea consumption. Xenobiotica 30: 317-326.
  • Dupont, S., N. Caffin, B. Bhandari and G.A. Dykes. 2006. In vitro antibacterial activity of Australian native herb extracts against food-related bacteria. Food Control 17: 929-932.
  • Farhoosh, R. G.A. Golmovahhed and M.H.H. Khodaparast. 2007. Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry 100: 231-236.
  • Gramza, A and J. Korczak. 2005. Tea constituents (Camellia sinensis L.) as antioxidants in lipid systems. Trends in Food Science and Technology 16: 351-358.
  • Halder, B., S. Pramanick, S. Mukhopadhyoy and A.K. Giri. 2005. Inhibition of benzo[a]pyrene induced mutagenicity and genotoxicity multiple test systems. Food and Chemical Toxicology 43: 591-597.
  • Jayaprakasha, G.K., T. Selvi and K.K. Sakariah. 2003. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Research International 36: 117-122.
  • Larger, P.J., A.D. Jones and C. Dacombe. 1998. Separation of tea polyphenols using micellar electrokinetic chromatography with diode array detection. Journal of Chromatography A 799: 309-320.
  • Luximon-Ramma, A., T. Bahorun, A. Crozier, V. Zbarsky, K.P. Datla, D.T. Dexter, O.I. Aruoma. 2005. Characterization of the antioxidant functions of flavonoids and proanthocyanidins in Mauritian black teas. Food Research International 38: 357-367.
  • Navas, P.B., A., Carrasquero-Durán and I. Flores. 2005. Effects of black tea, garlic and onion on corn oil stability and fatty acid composition under accelerated oxidation. International Journal of Food Science and Technology 40: 1-5.
  • Negi, P.S., A.S. Chauhan, G.A. Sadia, Y.S. Rohinishree and R.S. Ramteke. 2005. Antioxidant and antibacterial activities rhamnoides L.) seed extracts. Food Chemistry 92: 119- 124. (Hippophae
  • Nissen, L.R., D.V. Byrne, G. Bertelsen and L.H. Skibsted. 2004. The antioxidative activity of plant extracts in cooked pork patties as evaluated by descriptive sensory profiling and chemical analysis. Meat Science 68: 485- 495.
  • Pinelo, M., L. Manzocco, M.J. Nunez and M.C. Nicoli. 2004. Solvent effect on quercetin antioxidant capacity. Food Chemistry 88: 201-207.
  • Row, K.H. and Y. Jin. 2005. Recovery of catechin compounds from Korean tea by solvent extraction. Bioresource Technology 97: 790-793.
  • Satoh, E. 2005. Ethyl acetate extract from black tea prevents neuromuscular blockade by botulinum neurotoxin type A in vitro. International Journal of Food Science and Nutrition 56: 543-550.
  • Sharma, V., A., Gulati, S. D. Ravindranath and V. Kumar. 2005. A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. Journal of Food Composition and Analysis 18: 583-594.
  • Sun, T. and C. Ho. 2005. Antioxidant activities of buckwheat extracts. Food Chemistry 90: 743-749.
  • Turkmen, N., F. Sari, G. Polat and Y.S. Velioglu. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chemistry 99: 835-841.
  • Turkmen, N., Y.S. Velioglu, F. Sari and G. Polat. 2007. Effect of extraction conditions on measured total polyphenol contents and antioxidant and antibacterial activities of black tea. Molecules 12: 484-496.
  • Wang, H., G .J. Provan and K. Helliwell. 2000. Tea flavonoids: their functions, utilization and analysis. Trends in Food Science & Technology 11: 152-160.
  • Wheleer, D. and W. Wheleer. 2004. The medicinal chemistry of tea. Drug Development Research 61: 45-65.
  • Wu, S., G. Yen, B. Wang, C. Chiu, W. Yen, L. Chang and P. Duh. 2007. Antimutagenic and antimicrobial activities of pu-erh tea. Lebensmittel Wissenschaft und-Technologie 40: 506-512.
  • Yao, L., Y. Jiang, N. Datta, R. Singanusong, X. Liu, J. Duan, K. Raymont, A. Lisle and Y. Xu. 2004. HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia. Food Chemistry 84: 253-263.
  • Yilmaz, Y. 2006. Novel uses of catechins in foods. Trends in Food Science and Technology 17: 64-71.
  • Yu, J., M. Ahmedna and I. Goktepe. 2005. Effects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chemistry 90: 199-206.
  • Zhu, Y. H. Huang and Y. Tu. 2005. A review of recent studies in China on the possible beneficial health effects of tea. International Journal of Food Science and Technology 41: 333-340.