Mısır Danesinde Kalite Özelliklerinin NIR Yansıma Spektroskopisi ile Belirlenmesi
Bu çalışmada mısır ununda protein, yağ, karbonhidrat ve kül oranının NIRS ile tespitinde kullanılabilecek farklı kalibrasyon modellerinin karşılaştırılması amaçlanmıştır. Çalışmada 115 hibrit genotip ve 23 adet saf hatta ait toplam 138 örnek materyal olarak kullanılmıştır. Referans analizlerden elde edilen sonuçlara göre Kısmi En Küçük Kareler Regresyonu (PLSR) ve Çoklu Doğrusal Regresyon (MLR) yöntemleri kullanılarak farklı tahmin modelleri oluşturulmuştur. Oluşturulan modellerin (n=110) validasyon işlemi farklı genotipler (n=28) kullanılarak gerçekleştirilmiştir. Oluşturulan modellerin her ikisinde de en yüksek doğruluk protein oranında (rMLR=0.990 ve rPLSR=0.987) tespit edilmiştir. Diğer özellikler için MLR modeli PLSR modelinden (karbonhidrat için rMLR=0.801, rPLSR=0.755; yağ için rMLR=0.823, rPLSR=0.723; kül için rMLR=0.926, rPLSR=0.810) matematiksel modellere göre daha iyi sonuç vermiş olmasına karşın, dış validasyon işleminde PLSR modelinde yapılan tahminlerin MLR modeline göre hata payının düşük olduğu görülmüştür. Sonuçlar, NIR yöntemi ile protein oranının başarılı şekilde tahminlenebileceğini, karbonhidrat ve yağ gibi diğer özellikler için ise daha fazla çalışmalara ihtiyaç olduğunu ortaya koymuştur. Modellerde etkili olan dalga boylarına ait profil analizi, modele dahil edilen dalga boylarının regresyon katsıyaları düşük olduğunda tahmin gücünün de zayıf olduğunu göstermiştir. Ayrıca, kül ve yağ oranının, protein ve karbonhidrat oranına göre tarama yapılan bölgede daha fazla sayıda spektral bölge ile ilişkili olduğu belirlenmiştir.
Determination of Quality Parameters in Maize Grain by NIR Reflectance Spectroscopy
The objective of this study is to compare different calibration models that could be used in the analysis of protein, oil, carbohydrate and ash contents in maize flour by NIRS. A total of 138 samples were used from 115 hybrids and 23 inbreds in the study as material. Based on reference analysis results, different estimation models were developed using Partial Least Squares Regression (PLSR) and Multiple Linear Regression (MLR) methods. Validation procedure of these models (n=110) were accomplished using samples from different genotypes (n=28). In both of the developed models, the highest accuracy was attained for protein content (r=0.990 for MLR and r=0.987 for PLSR). For the other traits analyzed, although MLR model yielded better results based on mathematical evaluations (rMLR=0.801, rPLSR=0.755 for carbohydrate, rMLR=0.823, rPLSR=0.723 for oil, rMLR=0.926 and rPLSR=0.810 for ash), external validation suggested PLSR model provide a lower error rate than MLR. Results suggested that protein content could be successfully estimated, whereas, for some other traits, such as carbohydrate and oil ratios, it seems that there is still need for more studies before getting accurate measurements using NIR methods. Profile analysis regarding the wavelengths potent in the models showed that the estimation power declined when the regression coefficients of the wavelengths included in the model were low. Among the analyzed traits, ash and oil contents seemed to be related with more spectral regions within the scanned spectra than protein and carbohydrate.
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- AOAC (1990). Methods of the Association of Official
Analytical Chemists, Vol. II. 15th ed. Method No.
920.85. Arlington Virginia USA AOAC p. 780
- Bailleres H, Davrieux F & Ham-Pichavant F (2002).
Near infrared analysis as a tool for rapid screening
of some major wood characteristics in an
eucalyptus breeding program. Annals of Forest
Science 59: 479–490
- Başlar M & Ertugay M F (2011). Determination of
protein and gluten quality-related parameters of
wheat flour using near-infrared reflectance
spectroscopy (NIRS). Turkish Journal of
Agricultural and Forestry 35:139-144
- Baye T M, Pearson T C & Mark Settles A (2006).
Development of a calibration to predict maize seed
composition using single kernel near infrared
spectroscopy. Journal of Cereal Science 43: 236–
243
- Berardo N, Brenna O V, Amato A, Valotia P, Pisacanea
V & Mottoa M (2004). Carotenoids concentration
among maize genotypes measured by near infrared
reflectance spectroscopy (NIRS). Innovative Food
Science and Emerging Technologies 5: 393-398
- Buchanan B R, Baxter M A, Chen T-S, Qin X-Z &
Robinson P A (1996). Use of Near-Infrared
Spectroscopy to evaluate an active in a film coated
tablet. Pharmaceutical Research 13: 616-621
- Cozzolino D, Delucchi I, Kholi M & Vázquez D
(2006). Use of near infrared reflectance
spectroscopy to evaluate quality characteristics in
whole-wheat grain. Agricultura Técnica 66: 370-
375
- CWS Manual (2003). Sensologic Calibration Workshop
Version 2.02, Sensologic Gmbh, Germany
Deaville E R & Flinn P C (2000). (eds D.I. Givens, E.
Owen, R.F.E. Axford and H.M. Omed) NearInfrared (NIR) Spectroscopy: an Alternative
Approach for the Estimation of Forage Quality and
Voluntary Intake, Forage Evaluation in Ruminant
Nutrition, 301-320
- Diller M (2002). Investigations for the Development of
a NIRS-method for Potatoes in Organic Farming
with Special Reference to the Influence of the Year
and the Potato Line (in German). PhD Thesis.
Rheinische Friedrich-Wilhelms-Universitat, Bonn,
Germany
- Fülöp A & Hancsok J (2009). Comparison of
calibration models based on near infrared
spectroscopy data for the determination of plant oil
properties. Chemical Engineering Transactions 17:
445-450
- Gerhardt P, Murray R G E, Wood W A & Krieg N R
(1994). Methods for General and Molecular
Bacteriology, ASM, Washington DC. ISBN 1-
55581-048-9, p 518
- ICC (1980). ICC Standard No: 105/1. Method for the
Determination of Crude Protein in Cereals and
Cereal Products for Food and for Feed. Standard
Methods of the International Association for Cereal
Chemistry (ICC). Verlag Moritz Schafer. Detmold
- ICC (2000). Determination of Ash in Cereal and Cereal
Products. Standard Methods of the International
Association for Cereal Chemistry (ICC), ICC
Standard No: 104/1. Verlag Moritz Schafer.
Detmold
Jiang H Y, Zhu Y J, Wei L M, Dai J R, Song T M, Yan
- Y L & Chen S J (2007). Analysis of protein, starch
and oil content of single intact kernels by near
infrared reflectance spectroscopy (NIRS) in maize
(Zea mays L.). Plant Breeding 126:492-497
- Kahrıman F & Egesel C Ö (2011). Development of a
calibration model to estimate quality traits in wheat
flour using NIR (Near Infrared Reflectance)
spectroscopy. Research Journal of Agricultural
Sciences 43:392-400
- Martens H & Naes T (1992). Multivariate Calibration.
J. Wiley and Sons, Chichester, UK.pp:25
- Orman B A & Schumann R A (1991). Comparison of
near-infrared spectroscopy calibration methods for
the prediction of protein, oil, and starch in maize
grain. Journal of Agricultural Food and Chemisty
39: 883-886
- Osborne B G (2000). Near-infrared spectroscopy in
food analysis, In: Encyclopedia in analystical
Cehemistiry (Ed: R. A. Meyers), John Wiley Sons
- Pandorf J A & deMan J M (1990). Determination of oil
content of seeds by NIR: Influence of fatty acid
composition on wavelength selection. Journal of
American Oil Chemistry Society 67:473-482
- Pasquini C (2003) Near infrared spectroscopy:
Fundamentals, practical aspects and analytical
applications Journal of the Brazilian Chemical
Society 14:198–219.
- Rasco B A, Miller C E & King T L (1991). Utilization
of NIR spectroscopy to estimate the proximate
composition of trout muscle with minimal sample
pretreatment. Journal of Agricultural Food and
Chemistry 39: 67-72
- Rodriguez-Otero J L, Hermida M & Centeno J (1997).
Analysis of dairy products by near-infrared
spectroscopy: A review. Journal of Agricultural
Food and Chemisty 45:2815-2819
- Sandorfy C, Buchet R & Lachenal G (2007). Principles
of molecular vibrations for near-infrared
spectroscopy. In Near-Infrared Spectroscopy in
Food Science and Technology; Ozaki, Y., McClure,
W. F., Christy, A. A., Eds.; John Wiley and Sons,
Inc.: Hoboken, NJ, pp 11-46
- SAS Institute (1999). SAS V8 User Manual. SAS
Institue Cary NC
Shenk J S, Workman J J & Westerhaus M O (1992).
Application of NIR spectroscopy to agricultural
products. In: Burns, D.A., Ciurczak, E.W. (Eds.),
Handbook of Near-infrared Analysis, vol. 13.
Practical Spectroscopy Series, Marcel Dekker, New
York, pp. 383–431
- Siesler H W, Ozaki Y, Kawata S & Heise H M (2002).
Near-Infrared Spectroscopy. Principles,
Instruments, Applications. Wiley-VCH, Weinheim
Spielbauer G, Armstrong P, Baier J W, Allen W B,
- Richardson K, Shen B & Settles A M (2009).
- High-throughput near-infrared reflectance
spectroscopy for predicting quantitative and
qualitative composition phenotypes of individual
maize kernels. Cereal Chemistry 86(5): 556-564
- Tallada J G, Palacios-Rojas N & Armstrong P R
(2009). Prediction of maize seed attributes using a
rapid single kernel near infrared instrument.
Journal of Cereal Science 50:381–387
Wehling R L, Jackson D S, Hooper D G & Ghaedian A
- R (1993). Prediction of wet-milling starch yield
from corn by near-infrared spectroscopy. Cereal
Chemisty 70:720-723
- Welle R, Greten W, Müler T, Weber G & Wehrmann H
(2005). Application of near infrared spectroscopy
on-combine in corn grain breeding. Journal of Near
Infrared Spectroscopy 13:69-75