Figen ERASLAN, Şerife Evrim ARICI, İbrahim ERDAL, Zeliha KÜÇÜKYUMUK

Kiraz Anaçlarının in vitro Koşullarda Tuz Stresine Tolerans Mekanizmalarının Fizyolojik Parametreler ve Antioksidan Enzim İzoformları ile Belirlenmesi

Tuzluluk, bitkilerde gelişim ve verimi sınırlayan önemli abiyotik stres koşullarının başında gelmektedir. Kiraz anaçlarının tuz stresi toleransları ile ilgili bilgiler oldukça sınırlıdır. Bu çalışmada, ülkemizde yetiştirilen üç kiraz anacının; Colt Prunus avium x Prunus pseudocerasus , Gisela 5 Prunus cerasus x Prunus avium ve Maxma Prunus mahaleb x Prunus avium , tuz stresine tolerans mekanizmaları in vitro koşullarda araştırılmıştır. Kiraz anaçlarının tuz stresine toleranslarını belirlemek amacıyla Murashige ve Skoog MS ortamına 0, 25 ve 50 mM NaCl uygulanmıştır. Anaçlarda; sürgün gelişimi, lipid peroksidasyonu MDA , membran geçirgenliği MG , toplam antioksidan aktivitesi TAA , prolin içeriği, toplam klorofil içeriği, katalaz CAT, EC 1.11.1.6 ve peroksidaz POD, EC 1.11.1.7 antioksidan enzim izoformları ile Na ve Cl konsantrasyonları belirlenmiştir. Tuz stresi anaçların sürgün gelişimini ve toplam klorofil içeriğini kontrole göre azaltırken MDA içeriğini, MG, TAA ve prolin içeriğini artırmıştır. Anaçların stres koşullarında sürgün POD enzim aktivitesi artmış ve üç farklı POD izoformu elde edilmiştir. Maxma anacının POD aktivitesi diğer anaçların POD aktivitesinden daha düşük olmuştur. Anaçların CAT izoformlarında ise uygulamalar ve anaçlar arasında belirgin bir farklılık elde edilmemiştir. Bütün parametreler birlikte değerlendirildiğinde, NaCl stresine Maxma anacının hassas, Colt anacının orta derecede hassas ve Gisela 5 anacının da dayanıklı olduğu belirlenmiştir

Anahtar Kelimeler:

Enzim izoformu, Katalaz, Kiraz anaçları, Peroksidaz, Tuz stresi

Evaluation of Salt Tolerance Mechanisms with Physiological and Antioxidant Enzyme Isoform Parameters in in vitro Sweet Cherry Rootstocks

Abiotic stress such as salinity is an important factor that limits plant growth and performance. Information regarding the genotypic variation for salinity stress tolerance in sweet cherry rootstocks is limited. In this study, salinity tolerance mechanisms of three sweet cherry rootstocks, namely; Colt Prunus avium x Prunus pseudocerasus , Gisela 5 Prunus cerasus x Prunus avium and Maxma Prunus mahaleb x Prunus avium , grown widely in Turkey were investigated under in vitro condition. The three rootstocks were cultured in vitro on MS medium supplemented with 0, 25 and 50 mM sodium chloride NaCl . The Shoot growth, lipid peroxidation MDA , membrane permeability MP , total antioxidant activity TAA , proline, total chlorophyll contents, catalase CAT, EC 1.11.1.6 and peroxidase POD, EC 1.11.1.7 antioxidant enzyme isoforms of rootstocks were studied. Compared to the control, salinity resulted in a reduction in the shoot growth and total chlorophyll contents. Contrary to this, MDA contents, MP, TAA and proline contents were increased by salinity. Activity of POD in the shoot of rootstocks was increased and three different POD isoforms were exhibited under saline conditions. The activity of POD was lower in the Maxma than the Colt and Gisela 5 rootstock. Salinity did not significantly change the CAT isoforms of the rootstocks. Regarding the all parameters studied, the rootstocks can be classified to their salt tolerance as sensitive Maxma , moderately sensitive Colt and resistant Gisela 5

Keywords:

Catalase, Enzyme isoform, Peroxidase, Salinity stress, Sweet cherry rootstocks,

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