Bülent BAYRAKTAR, Emre TEKCE, Vecihi AKSAKAL, Ciğdem TAKMA, Fatma Gülten BAYRAKTAR, Bülent ŞENGÜL

Effects of Race, Gender, Body Condition Score and Pregnancy on Serum Apelin Levels in Ewe

Apelin is an important adipokine hormone that is released from adipose tissue, which is considered as the energy store of the body, which plays a role in many physiological processes in the body, as well as cardiovascular, immune functions and energy, nutrients and fluid metabolism. In this study, it was aimed to determine the effect of lactation, pregnancy and gender on apelin hormone levels in blood serums belonging to different races of ewe. In the present study, the hormone levels of the pregnant, non-pregnant ewe and rams of the Akkaraman Kangal and Morkaraman races were thin, with different body condition scores (<2, 3 to 3.5 and ≥4). Apelin hormone level was determined by ELISA technique in blood serum samples of ewe's Jugular vein. It was determined that there was a difference in body scores between races and the interaction between race and body score was important (P<0.05). Apelin level in ewe in lactation and pregnant ewe did not change according to body condition scores (P>0.05). In terms of apelin, gender and body condition score, race and gender, and body condition score interactions were found to be significant.

Keywords:

Ewe, Apelin, Race, Gender, Pregnancy, ELISA,

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AA., K. (2006). Koyunların gebelik toksemisi.“Geviş Getiren Hayvanların İç Hastalıkları”. 2. Baskı, Ed; Gül Y, Medipres, Malatya. Ağaoğlu ZT, Y. A. (2006.). Koyunların ketozisi, “Geviş Getiren Hayvanların İç Hastalıkları”, 2. Baskı, Ed; Gül Y, Medipres, Malatya. . AOAC. (2005). Official Methods of Analysis of AOAC International. 18th ed. Rockville, MD, USA: Association of Official Analytical Chemists. Bełtowski, J. (2006). Apelin and visfatin: unique. Medical Science Monitor, 12(6), RA112-RA119.
Biçer, O. (1991). Koyunlarda vücut kondüsyon puanlaması ve koyun yetiştiriciliğinde önemi. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 6(4), 81-88.
Cheng, B., Chen, J., Bai, B., & Xin, Q. (2012). Neuroprotection of apelin and its signaling pathway. Peptides, 37(1), 171-173.
Choe, W., Albright, A., Sulcove, J., Jaffer, S., Hesselgesser, J., Lavi, E., . . . Kolson, D. L. (2000). Functional expression of the seventransmembrane. J Neurovirol, 6(1), 61-69.
Cobellis, L., De Falco, M., Mastrogiacomo, A., Giraldi, D., Dattilo, D., Scaffa, C., . . . De Luca, A. (2007). Modulation of apelin and APJ receptor in normal and preeclampsia-complicated placentas. Histology and histopathology, 22(1-3), 1-8.
Edinger, A. L., Hoffman, T. L., Sharron, M., Lee, B., Yi, Y., Choe, W., . . . Faulds, D. (1998). An orphan seven-transmembrane domain receptor expressed widely in the brain functions as a coreceptor for human immunodeficiency virus type 1 and simian immunodeficiency virus. Journal of virology, 72(10), 7934-7940.
Habata, Y., Fujii, R., Hosoya, M., Fukusumi, S., Kawamata, Y., Hinuma, S., . . . Kurokawa, T. (1999). Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1452(1), 25-35. Heinonen, M., Purhonen, A., Miettinen, P., Pääkkönen, M., Pirinen, E., Alhava, E., . . . Herzig, K. (2005). Apelin, orexin-A and leptin plasma levels in morbid obesity and effect of gastric banding. Regulatory peptides, 130(1-2), 7-13.
Kasai, A., Kinjo, T., Ishihara, R., Sakai, I., Ishimaru, Y., Yoshioka, Y., . . . Maeda, S. (2011). Apelin deficiency accelerates the progression of amyotrophic lateral sclerosis. PloS one, 6(8), e23968.
Katugampola, S., & Davenport, A. (2003). Emerging roles for orphan G-protein-coupled receptors in the cardiovascular system. Trends in pharmacological sciences, 24(1), 30-35.
Kourtis, A., Gkiomisi, A., Mouzaki, M., Makedou, K., Anastasilakis, A. D., Toulis, K. A., . . . Agorastos, T. (2011). Apelin levels in normal pregnancy. Clinical endocrinology, 75(3), 367-371.
Leeper, N. J., Tedesco, M. M., Kojima, Y., Schultz, G. M., Kundu, R. K., Ashley, E. A., . . . Quertermous, T. (2009). Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation. American journal of physiology-heart and circulatory physiology, 296(5), H1329-H1335. Medhurst, A. D., Jennings, C. A., Robbins, M. J., Davis, R. P., Ellis, C., Winborn, K. Y., . . . Bolaky, J. E. (2003). Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin. Journal of neurochemistry, 84(5), 1162-1172.
Reaux, A., De Mota, N., Skultetyova, I., Lenkei, Z., El Messari, S., Gallatz, K., . . . Llorens‐Cortès, C. (2001). Physiological role of a novel neuropeptide, apelin, and its receptor in the rat brain. Journal of neurochemistry, 77(4), 1085-1096. Sato K, K. Y., Takahashi T, Katoh K,. (2009). Effects of i.v. administration of apelin on endocrine in sheep and goats,. XIth International Symposium on Ruminant Physiology.
Taheri, S., Murphy, K., Cohen, M., Sujkovic, E., Kennedy, A., Dhillo, W., . . . Bloom, S. (2002). The effects of centrally administered apelin-13 on food intake, water intake and pituitary hormone release in rats. Biochemical and biophysical research communications, 291(5), 1208-1212.
Tang, S.-Y., Xie, H., Yuan, L.-Q., Luo, X.-H., Huang, J., Cui, R.-R., . . . Liao, E.-Y. (2007). Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways. Peptides, 28(3), 708-718.
Telejko, B., Kuzmicki, M., Wawrusiewicz-Kurylonek, N., Szamatowicz, J., Nikolajuk, A., Zonenberg, A., . . . Wilczynski, J. (2010). Plasma apelin levels and apelin/APJ mRNA expression in patients with gestational diabetes mellitus. Diabetes research and clinical practice, 87(2), 176-183.
Thomson, E., & Bahhady, F. (1988). A note on the effect of live weight at mating on fertility of Awassi ewes in semi-arid north-west Syria. Animal Science, 47(3), 505-508.
Trayhurn, P., Bing, C., & Wood, I. S. (2006). Adipose tissue and adipokines—energy regulation from the human perspective. The Journal of nutrition, 136(7), 1935S-1939S.
Zeng, X. J., Yu, S. P., Zhang, L., & Wei, L. (2010). Neuroprotective effect of the endogenous neural peptide apelin in cultured mouse cortical neurons. Experimental cell research, 316(11), 1773-1783.