Korhan ARSLAN, Fadime ÖZDEMİR, Esma Gamze İLGAR, Bilal AKYÜZ

Döl Tutma Problemi Olan Holştayn Irkı Sığırlarda Sitogenetik ve Moleküler Genetik Taramalar

Sığır yetiştiriciliğinde repeat breeder sendromu olarak adlandırılan döl tutma problemi önemli bir sorundur. Holştayn ırkı sığırlarda bu problemin ortaya çıkmasında bakım-beslemenin yanı sıra Robertsonian translokasyon ve faktör XI yetmezliği FXID olarak adlandırılan genetik bozukluklarında rolü olduğu düşünülmektedir. Bu çalışmada Kayseri ilinde bir çiftlikte bulunan repeat breederlı Holştayn ırkı ineklerde Robertsonian translokasyon ve FXID varlığının araştırılması amaçlanmıştır. Yapılan çalışmada, repeat breeder tanısı konan 62 baş Holştayn ırkı inek incelenmiştir. Kromozomal inceleme sonucunda 62 hayvanın 58’inin normal karyotip 2n= 60 diploid , dördünün ise en sık görülen 1;29’dan farklı dört tip Robertsonian translokasyon [rob 1;21 , rob 23;26 , rob 24;26 , rob 26;29 ] profiline sahip olduğu saptanmıştır. Yapılan PCR analizi sonucunda, incelenen 62 örneğin hiçbirinde FXID’e neden olan mutasyona ait bant görüntüsü gözlenmemiştir. Çalışma sonunda, döl tutma problemi bulunan sığırlarda sitogenetik ve moleküler genetik taramaların yapılmasının kalıtsal nedenlerle gelişen repeat breeder sendromu gösteren damızlık adayların belirlenerek damızlık dışı bırakılmasına ve bu sayede özellikle damızlık yetiştiren işletmeler için çözüm yollarının aranmasına katkı sağlayacağı düşünülmüştür

Anahtar Kelimeler:

FXID, Holştayn, Repeat breeder, Robertsonian translokasyon

Cytogenetic and Molecular Genetic Screening in Holstein Cattle Breed which Showing Repeat Breeding Problems

The infertility problem named Repeat Breeder Syndrome is an important issue in cattle breeding. Beside maintenance and feed techniques, some genetic disorders such as Robertsonian Translocation and factor XI deficiency FXID are thought to be involved some problems in Holstein cattle. In this study, it was aimed to investigate the presence of Robertsonian Translocation and FXID in repeat breeder Holstein cows which grown a farm that located in the province of Kayseri. In the study 62 female Holstein cattle with Repeat Breeder Syndrome were examined. Chromosomal examination results indicated that 58 of 62 cows had a normal karyotype 2n= 60 diploid and four had different of Robertsonian translocation [Rob 1, 21 , rob 23, 26 , Rob 24, 26 , Rob 26, 29 ] profiles. None of the 62 cows was displayed the band of mutation which is the cause of FXID. As a result of the study it was determined molecular and cytogenetical screening of cows with a fertility problem could contribute to understand the reasons of repeat breeder syndrome and could be useful for the breeders to select the female breeding candidates and finding the solution for this problem.

Keywords:

FXID, Holstein, Repeat breeder, Robertsonian translocation,

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