Murat ARDIÇ, Murat OLGUN, Onur KOYUNCU, Engin TAKIL, Zekiye BUDAK BAŞÇİFTÇİ, Güleray AĞAR, N. Gözde AYTER, Metin TURAN

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Genetic Divergence in Some Barley (Hordeum vulgare L.) Genotypes by RAPD and ISSR Analyses

Özet: Bu çalışmada RAPD ve ISSR analizleri kullanılarak, arpa çeşitlerindeki genetik farklıların ortaya konması amaçlanmıştır. ISSR ve RAPD analizleri sonucunda, çeşitlerin primer bantlarının; RAPD analizinde 4 ile 11, ISSR analizine ise 7-11 arasında değiştiği belirlenmiştir. Primer ve primer kombinasyonlarının çoğu polimorfik bantların RAPD analizinde OPA-04, OPA-13 ve OPH-17; ISSR analizinde ise UBC-808, UBC-820 ve UBC-872 olduğu belirlenmiştir. Yine elde edilen sonuçlara göre ortalama polimorfik oran % 86.5 olarak tespit edilmiştir. Arpa çeşitleri geliştirilmesi açısından, çeşitlerin genetik farklılıkları belirlenmiş olup, genetik varyabilitenin ortaya konmasına da önemli katkı sağlamıştır. Sonuç olarak RAPD ve ISSR analizleri arpada genetik çeşitliliğin belirlenmesinde faydalı bir yöntem olup, çeşitlerin genetik ilişkisinin ve farklılıklarının belirlenebilmesinde güvenilir bir yöntem olarak kullanılabileceği ortaya konmuştur.

Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Belirlenmesi

The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.
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Süleyman Demirel Üniversitesi Ziraat fakültesi Dergisi-Cover
  • ISSN: 1304-9984
  • Yayın Aralığı: Yılda 2 Sayı
  • Yayıncı: Süleyman Demirel Üniversitesi Ziraat Fakültesi
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