Mustafa ÖZTOP, Kenan ÇINAR, Emel DEMİRBAĞ

Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Pek çok fonksiyona katılan glikokonjugatlar, birçok hücre tipi tarafından üretilir. Bu çalışmada Oscar balığı (Astronotus ocellatus) solungaçlarındaki glikokonjugat varlığının beş horseradish peroksidaz ile konjuge lektin kullanarak araştırılması amaçlandı. Bu çalışma, solungaç epitel hücrelerinin Canavalia ensiformis agglutinin (Con A) ve Arachis hypogaea agglutinin (PNA) ile reaksiyona girdiğini ortaya çıkardı. Solungaç primer lamellerinin epitel hücrelerinde Con A’ya karşı güçlü reaksiyon görüldü. Primer ve sekonder lamel epitel hücreleri ve eozinofilik granüler hücreler, değişen yoğunlukta PNA bağlanmasına sahipti. Primer lamellerin bağ dokusunda Ulex europaeus agglutinin-I (UEA-I) pozitif eozinofilik granüler hücreler gözlendi. Solungaç primer filamentinin epitel hücreleri, çeşitli yoğunluklarda Con A reaksiyonuna sahipti. Yüzeysel epitel hücreleri PNA’ya karşı çeşitli derecelerde reaksiyon göstermekteydi. Sekonder lamellerin epitel hücreleri Con A ve PNA pozitif reaksiyona sahipti. Bu sonuçlar, glikokonjugat dağılımının türler arası varyasyonlar gösterebileceğine işaret etmektedir, bu da Oscar balık solungaçlarının epitelyal mukoza hücrelerinde doğal olarak oluşan glikokonjugatların yüksek heterojenitesinin, glikokonjugatların çeşitli fonksiyonel rollerine dayandırılabileceğini düşündürmektedir.

Anahtar Kelimeler:

Lektin, Oskar balığı, Glikokonjugat, Astronotus ocellatus

Distribution of Glycoconjugates in the Gills of Oscar Fish (Astronotus ocellatus)

Glycoconjugates involved in many functions are produced by many cell types. This study aimed to investigate the glycoconjugate expression in the Oscar fish (Astronotus ocellatus) gills using five horseradish peroxidase-conjugated lectins. This study revealed that gill epithelial cells react with Canavalia ensiformis agglutinin (Con A) and Arachis hypogaea agglutinin (PNA). Strong reaction to Con A was seen in the epithelial cells of the gill primary lamellae. Epithelial cells of primary and secondary lamellae and eosinophilic granular cells had varying intensity of PNA binding. Ulex europaeus agglutinin-I(UEA-I) positive eosinophilic granular cells were observed in the connective tissue of primary lamellae. Epithelial cells of the gill primary filament had Con A reaction in varying intensities. Superficial epithelial cells reacted with PNA in various degrees. Epithelial cells of the secondary lamellae had Con A and PNA positive reaction. These results indicate that glycoconjugate distribution may show interspecific variations, suggesting that the high heterogeneity of naturally occurring glycoconjugates in the epithelial cells of Oscar fish gills might be attributed to the various functional roles of glycoconjugates.

Keywords:

Lectin, Oscar fish, Glycoconjugate, Astronotus ocellatus,

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