The Effect of PRGF on Healing of Clamp-Induced Tendinopathy in Animals

Objectives: To investigate the effect of PRGF on the healing of the tendon damage in the Achilles tendinopathy model. Material and Methods: A preliminary study was designed to obtain more apparent tendinopathy changes in the subculture. Histopathologically, second level compression with a clamp for 1 minute followed by releasing for 3 minutes applying the same mechanical stimulus procedure for four times, was defined as the optimal compression mode for the most optimal damage in terms of tendinopathy. Thirty-four female Sprague Downey type rats were divided into five separate groups and clamped tendon damage was formed on unilateral (left) Acchilles tendons. The first group was a control group (Kontrol: CN); in the second group tissue specimens were taken from the rats immediately after being clamped (K-0); from the group 3, tissue samples were taken 1 week after clamping (K-7). Immediately after the tendinopathy model was applied to the animals in the fourth group (PRGF), the PRGF injection was performed to the subjects from the blood that was obtained from the control group, while an isotonic saline solution was injected into the subjects in the fifth group (SF), and tissue samples from both groups were obtained 1 week after. Results: The samples were examined by means of a transmission electron microscope. In the K-0 group among the collagenous fibers, vacuoles are observed in the cytoplasm of the edematous areas and fibrocytes, and in comparison, with K-7 and SF group there was a significant increase in fibroblast and collagen fiber synthesis in the PRGF-treated group. Furthermore, fibroblast and new collagen synthesis were observed to be increased in K-7 and SF groups. An intense increase in organelle levels was observed in PRGF group fibroblasts. Conclusion: This data suggests that PRGF may provide a better healing response in tendons with limited healing and circulation, which enhances the activation in the cells at the beginning of the healing process.

Klemp ile Tendinopati Oluşturulan Hayvanlarda PRGF Uygulamasının İyileşmeye Etkisi

Amaç: PRGF uygulamasının Aşil tendinopati modelinde tendon hasarı iyileşmesindeki etkisini incelemek Gereç ve Yöntemler: Bu çalışma öncesinde Aşil tendonunda tendinopatik değişikliklerin daha belirgin ortaya çıkmasını sağlamak amacıyla yapılan ön çalışmada; klemp ile ikinci dişli seviyesinde 1 dakika sıkıştırmayı takiben 3 dakika serbest bırakma şeklinde yapılan dört sıkıştırmanın tendinopati açısından en optimal hasarı yarattığı histopatolojik olarak gösterildi. 34 adet dişi Sprague Downey tipi sıçan, beş ayrı gruba ayrılarak tek taraflı (sol) aşil tendonlarına klemp ile tendon hasarı oluşturuldu. İlk gruba herhangi bir şey uygulamaksızın (Kontrol:CN) doku örnekleri alınırken, 2. gruptaki sıçanlardan klemp ile sıkıştırıldıktan hemen sonra (K-0), 3. gruptakilerden ise klemp ile sıkıştırıldıktan 1 hafta sonra (K-7) doku örnekleri alındı. 4. gruptaki hayvanlara (PRGF) tendinopati modeli uygulandıktan hemen sonra kontrol grubu deneklerden alınan kandan hazırlanmış PRGF enjeksiyonu yapıldı ve 1 hafta sonra doku örnekleri elde edilirken, son gruptaki hayvanlara (SF) klemp ile sıkıştırıldıktan hemen sonra serum fizyolojik enjeksiyonunu takiben 1. haftada feda edilerek doku örnekleri alındı. Bulgular: Örneklerin transmisyon elektron mikroskop ile yapılan incelemesinde; K-0 grubunda kollajen lifler arasında yer yer ödemli sahalar ve fibrositlerin sitoplazmalarında vakuoller izlenirken, K-7 ve SF grupları ile karşılaştırıldığında; PRGF uygulanan grupta fibroblastlarda ve kollajen lif sentezinde belirgin artış olduğu gözlendi. Bununla birlikte K-7 ve SF gruplarında da fibroblast ve yeni kollajen sentezi artmış olarak izleniyordu. PRGF grubu fibroblastlarında organel düzeyinde yoğun bir artış gözlendi. Sonuç: Bu veriler PRGF’in iyileşmeye erken dönemde katkısının hücrelerdeki aktivasyonu arttırarak gerçekleştirdiği, kısıtlı iyileşme ve döngüye sahip tendonlarda daha iyi bir iyileşme yanıtı sağlayabileceği şeklinde yorumlandı.

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