Investigation of the Effects of Maresin-1 on Testicular Ischemia Reperfusion Induced Oxidative Stress

Objective: The purpose of this study is to examine the protective effects of maresin 1 on testicular injury induced by ischemia reperfusion.Methods: 24 Sprague-Dawley male rats were divided into 3 groups. The groups are planned as follows; sham, ischemia reperfusion and ischemia reperfusion+maresin 1 groups. The spermatic cord was detected and clamped for 2 hours to establish the prompt. After 2 hours, the clamp was removed and testicular reperfusion was achieved for 2 hours. At the end of the reperfusion phase, testicular tissues were taken and total antioxidant capacity (TAC), total oxidant capacity (TOC), superoxide dismutase (SOD), malondialdehyde (MDA) and myeloperoxidase (MPO) levels were determined by spectrophotometric method.Results: In the ischemia-reperfusion group, TOS and MDA and MPO are increased, the levels of TAS and SOD molecules are decreased. TOS and SOD levels are increased and TAS, MDA and MPO levels are decreased in the Maresin-1 treatment group.Conclusion: These results show us that a single dose of maresin-1 application is effective against oxidative damage caused by ischemia reperfusion.

Testiküler İskemi Reperfüzyon ile İndüklenen Oksidatif Strese Maresin-1’in Etkilerinin Araştırılması

Amaç: Bu araştırmanın amacı, maresin 1'in iskemi reperfüzyonun indüklediği testis hasarı üzerindeki koruyucu etkilerini incelemektir.Gereç ve Yöntem: 24 Sprague-Dawley erkek sıçanlar 3 gruba ayrıldı. Gruplar şu şekilde planlanmıştır; sham, iskemi reperfüzyon ve iskemi reperfüzyon+ maresin 1 grupları. Spermatik kord tespit edildi ve istemi oluşturmak için 2 saat süre ile klempendi. İki saati takiben klemp çıkarıldı ve 2 saat boyunca testis reperfüzyonu sağlandı. Reperfüzyon aşamasının sonunda, testis dokuları alınarak total antioksidan kapasite (TAK), total oksidan kapasite (TOK), süperoksit dismütaz (SOD), malondialdehit (MDA) ve myeloperoksidaz (MPO) düzeyleri spektrofoto-metrik yöntemle belirlendi.Bulgular: İskemi-reperfüzyon grubunda TOS ve MDA ve MPO arttı, TAS ve SOD moleküllerinin seviyeleri azaldı. Maresin-1 tedavi grubunda TOS ve SOD düzeyleri artarken ve TAS, MDA ve MPO düzeyleri azaldı.Sonuç: Bu sonuçlar bize, tek doz maresin-1 uygulamasının, iskemi reperfüzyonunun neden olduğu oksidatif hasara karşı etkili olduğunu göstermiştir.

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Kaynak Göster

Southern Clinics of Istanbul Eurasia
  • ISSN: 2587-0998
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2017

1.1b387

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