Songül DOĞANAY, Şeyma TRABZON, Nurten BAHTİYAR, Derya GÜZEL, Ayla EREN ÖZDEMİR, Arzu ŞAHİN

Streptozotosin ile İndüklenmiş Diyabetik Sıçanlarda Melatoninin Antioksidan Aktivitesi; Kan ve Karaciğer Dokusunda

AMAÇ: Bu çalışmada Streptozotosin (STZ) ile deneysel tip 1 diyabet oluşturulan sıçanların kan ve karaciğer dokularında oluşan oksidatif hasara karşı melatonin takviyesinin koruyucu etkisinin araştırılması amaçlandı. GEREÇ ve YÖNTEM: Kırk adet yetişkin Spraque Dawley erkek sıçan; grup 1- kontrol grubu, grup 2-diyabet oluşturulan grup, grup 3- melatonin takviye grubu ve grup 4- diyabet ve melatonin takviye grubu olmak üzere dört gruba ayrıldı. Sıçanlara tek doz 60 mg/kg STZ İntraperitoneal (i.p.) olarak uygulanması ile diyabet oluşturuldu. Melatonin takviyesi ise 10 mg/kg/gün dozunda subkutan olarak (s.c.) 6 hafta boyunca uygulandı. Deney sonunda alınan karaciğer ve serum örneklerinde; glutatyon (GSH), katalaz (CAT) ve malondialdehit (MDA) düzeyleri spektrofotometrik yöntem kullanılarak ölçüldü. BULGULAR: Çalışma sonuçları diyabetik hayvanların serum ve karaciğer dokularında MDA düzeylerinin arttığını gösterdi. Melatonin uygulamasının her iki dokuda da MDA düzeylerini azaltıp, GSH ve CAT düzeylerini artırdığı veya düşmesini engelleyerek kararlı bir şekilde kalmasını sağladığı görüldü (p

Anahtar Kelimeler:

glutatyon, katalaz, melatonin, oksidatif stres, tip 1 diyabet

Antioxidant Activity of Melatoninin in Streptozotocin-İnduced Diabetic Rats; Blood and Liver Tissue

OBJECTIVE: In this study, it was aimed to investigate the antioxidant protective effect of melatonin supplementation against oxidative damage in blood and liver tissues of rats with experimental type 1 diabetes with Streptozotocin (STZ). MATERIALS AND METHODS: Forty adult Spraque Dawley male rats; Group 1-Control group, Group 2-diabetes group, Group 3- melatonin supplement group and Group 4- diabetes and melatonin supplement group were divided into four groups. Diabetes was induced in rats by administering a single dose of 60 mg / kg STZ intraperitoneally. Melatonin supplementation was administered at a dose of 10 mg / kg / day subcutaneously for 6 weeks. Glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) levels were measured spectrophotometrically in the liver and serum samples taken at the end of the experiment. RESULTS: The results of the study showed that MDA levels increased in the serum and liver tissues of diabetic animals. It was observed that melatonin administration decreased MDA levels in both tissues, increased GSH and CAT levels or kept them stable by preventing it from falling (p

Keywords:

melatonin, oxidative stress, Catalase, gutathione, type 1 Diabetes,

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