Mehmet Hanifi TANYERİ, Mehmet Emin BÜYÜKOKUROĞLU, Pelin TANYERİ, Aykut ÖZTÜRK, Rümeysa KELEŞ, Şeyma Nur BAŞARIR BOZKURT, Oguz MUTLU

Oksibutininin Farelerde Pasif Sakınma ve Morris Su Tankı Testlerinde Öğrenme Ve Bellek İşlevleri Üzerine Etkileri

Amaç: Aşırı aktif mesane (AAM) çocukluk çağı inkontinans nedenlerinin çoğunluğunu oluşturmaktadır. Oksibutinin, AAM tedavisinde çocuklarda sıklıkla kullanılan antimuskarinik bir maddedir. Çalışmamızın amacı antimuskarinik ilaçlardan biri olan oksibutininin öğrenme ve bellek üzerine etkilerinin araştırılmasıdır. Gereç ve Yöntemler: Farelerde Pasif Sakınma (PA) Testi ve Morris Su Tankı (MWM) Testini kullanarak oksibutinin’in öğrenme ve hafıza üzerindeki etkilerini değerlendirdik. Bulgular: Oksibutinin tedavisinin (1 mg/kg, 2 mg/kg ve 4 mg/kg) kontrole göre ikinci gün tutma süresinde önemli bir farklılık göstermermezken, skopolamin uygulanmış farelerde oksibutinin (4 mg/kg) retansiyon süresini önemli ölçüde uzatmıştır. MWM Testinde, oksibutinin (1 mg/kg, 2 mg/kg ve 4 mg/kg) hedef kadranda geçirilen süre üzerinde hiçbir etkisi yoktur. Skopolamin (0,6 mg/kg) tek başına hedef kadranda harcanan süreyi önemli ölçüde azalttı, ancak oksibutinin (4 mg/kg), skopolamin ile tedavi edilen farelerde hedef kadranda geçirilen süreyi önemli ölçüde uzattı. Ayrıca, skopolamin, kaçış platformuna olan ortalama mesafeyi önemli ölçüde artırırken, oksibutinin (4 mg/kg), skopolamin ile tedavi edilen farelerde kaçış platformuna olan ortalama mesafeyi önemli ölçüde azalttı. Sonuç: Çalışmamızda oksibutinin öğrenme ve belleği etkilemezken, bozulmuş öğrenme ve bellek üzerine olumlu etkileri olduğu görülmüştür. Bu çalışmanın sonuçları AAM'li hastalarda oksibutinin kullanımının öğrenme ve hafızayı etkilemediğini göstermektedir.

Anahtar Kelimeler:

oksibutinin, skopolamin, öğrenme, bellek, fare

The Effects of Oxybutynin on Learning and Memory Functions In Passive Avoidance and Morris Water Maze Tests In Mice

Objective: Overactive bladder (OAB) constitutes the majority of childhood incontinence causes. Oxybutynin is an antimuscarinic agent frequently used for children in the treatment of OAB. This study aimed to investigate the effects of an antimuscarinic drug oxybutynin on learning and memory. Materials and Methods: We assessed the effects of oxybutynin on learning and memory functions using the Passive Avoidance (PA) and the Morris Water Maze (MWM) Test in mice. Results: Oxybutynin treatment (1 mg/kg, 2 mg/kg, and 4 mg/kg) did not show a significant difference in the retention time on the second day compared to the control and oxybutynin (4 mg/kg) significantly prolonged the retention time in scopolamine-treated mice. In the MWM Test, oxybutynin (1 mg/kg, 2 mg/kg, and 4 mg/kg) has no effect on the time spent in the target quadrant. Scopolamine (0.6 mg/kg) alone significantly reduced time spent in the target quadrant, but oxybutynin (4 mg/kg) significantly prolonged time spent in the target quadrant in scopolamine-treated mice. Also, scopolamine significantly increased the mean distance to the escape platform, while oxybutynin (4 mg/kg) significantly decreased the mean distance to the escape platform in scopolamine-treated mice. Conclusion: In our study, oxybutynin did not affect learning and memory, but it plays a role ameliorating the learning and memory deficits. The results of this study show that the use of oxybutynin in patients with OAB does not affect learning and memory.

Keywords:

oxybutynin, scopolamine, learning, memory, mice,

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