Recep Demir, Ömer Atış, Lütfi Özel, Hasan Doğan, Gökhan Özdemir, Hızır Ulvi

Genç İskemik Stroklu Hastalarda Protrombotik Gen Polimorfizmleri ve Strok Risk Faktörlerinin Belirlenmesi

Genç İskemik Stroklu Hastalarda Protrombotik Gen Polimorfizmleri ve Strok Risk Faktörlerinin BelirlenmesiAmaç: Genç iskemik inmeli hastalarda MTHFR C677T, Protrombin G20210A ve Faktör V Leiden (G1691A) gen polimorfizmlerinin bir risk faktörü olup olmadığının araştırılmasıdır.Gereç ve yöntem: Çalışmaya, 50 yaş altında iskemik strok geçirmiş 142 hasta ve 101 kontrol grubu (sağlıklı poliklinik hastası) toplam 243 kişi dahil edildi. Hasta ve kontrol grubundan EDTA'lı tüplere periferik venöz kan örnekleri alındı ve genomik deoksiribonükleik asitler (DNA) elde edildi. Faktör V Leiden, FII G20210A ve MTHFR C677T polimorfizmleri standart Real Time - PCR protokolü ile genotiplemesi yapıldı. Hastaların yatışının 1. ve 3. günleri beyin görüntülemesi yapıldı. İstatistiksel olarak ki-kare, anova testi ve logistik regresyon analizi kullanıldı.Bulgular: Çalışma, 142'si (58,4%) arteryal iskemik strok hastalarından ve 101'i (41,6%) sağlıklı kontrol grubundan oluşmaktaydı. Arteryel iskemik strok hastalarda kontrol grubuna göre daha fazla MTHFR C677T mutasyonu vardı (p

Anahtar Kelimeler:

MTHFR C677T, Faktör V Leiden, FII G20210A, risk faktörleri, genç strok

Determination of Prothrombotic Gene Polymorphisms and Stroke Risk Factors in Young Ischemic Stroke Patients

Objective: To examine whether MTHFR C677T, Prothrombin G20210A and Factor V Leiden (G1691A) gene polymorphisms create a risk factor or not in young ischemic stroke patients.Materials and method: A total of 243 people under the age of 50 were included in the study 142 of whom had ischemic stroke and 101 of whom comprised the healthy control group (healthy polyclinic patients). Peripheric venous blood samples were taken from the patient and control group into EDTA tubes and genomic deoxyribonucleic acids (DNA) were obtained. Factor V Leiden, FII G20210A and MTHFR C677T polymorphisms genotyping was carried out via standard Real Time - PCR protocol. Brain imaging process was carried out on the 1st and 3rd days of the hospitalization of the patients. Statistically, chi-square, anova test and logistic regression analysis were used. Results: The study consisted of 142 (58,4%) patients with arterial ischemic stroke and 101 (41,6%) healthy controls. The MTHFR C677T mutation in arterial ischemic stroke patients was greater in comparison with the control group (p

Keywords:

MTHFR C677T, Faktör V Leiden, FII G20210A, risk factors, young stroke,

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