Madeeha AKRAM, Ijaz SALEEM, Muhammad FARHAB, Zubair LUQMAN

Immunomodulatory Effects of Moringa Oleifera Leaf Meal (MOLM) against Newcastle Disease in Broilers

We checked the efficacy of Moringa oleifera leaf meal (MOLM) for immunomodulatory effects in broilers. 144 birds that were divided into 4 groups having 12 birds each that were further subdivided into groups of 12. Such that treatment groups were D; receiving 0%, divided into replicates D1, D2, D3 while similar divisions of group A, B and C received 2, 4 and 6 % of MOLM infused in their feed as an additive supplement. The birds were slaughtered at 14th, 21th and 35th day and blood as well as serum was collected for the sake of hematological parameters and to check the antibody titer using HA/HI checking for effectiveness of immune system at producing antibodies against Newcastle Disease, which the birds were vaccinated against. Diet B has maximum weight gain fed while Diet D has lowest weight gain, which was a control group. Maximum feed intake was with diet A while lowest feed intake with diet D. Improved FCR was with diet B whereas the highest feed conversion ratio (FCR) was observed in broilers fed diet without Moringa oleifera supplementations. It was concluded that Moringa infused in feed does enhance immune parameters of broilers, making them better at combating diseases, making Moringa a viable dietary supplement for commercial poultry. It was also found that the optimal dose of Moringa in MOLM is 4 g/kg feed.

Etlik Piliçlerde Moringa Oleifera Yaprak Ununun (MOLM) Newcastle Hastalığına Karşı İmmünomodülatör Etkileri

Etlik piliçlerde immünomodülatör etkiler açısından Moringa oleifera yaprak ununun (MOLM) etkinliğini kontrol ettik. Çalışmada herbirinde 12 hayvan olan 4 grup oluşturulmuş, toplam 144 hayvan, 12 altgruba ayrılmıştır. Denemede Moringa oleifera yaprak ununun (MOYU) farklı düzeylerdeki (%0, 2, 4 ve 6) etkisi araştırılmıştır. Hayvanlar 14, 21. Ve 35.gün kesilmiş, hematolojik parametrelerin tespiti için kanları alınarak serumları çıkartılmıştır. Ayrıca HA / HI kullanılarak antikor titresinin bağışıklık sisteminin Newcastle Hastalığına karşı antikor üretmedeki etkinliğini kontrol edilmiştir. Çalışmada Diet b, maksimum canlı ağırlık kazancına ulaşırken; Diet D, en düşük canlı ağırlık kazancına sahip olmuştur. Maksimum yem tüketimi Diet A grubunda; en düşük yem tüketimi de Diet D grubunun yemiyle beslenen hayvanlarda görülmüştür. Diet B’de yemden yararlanma oranı iyileşirken; Moringa oleifera katılmayan grupta en yüksek yemden yararlanma oranı tespit edilmiştir. Yemlere Moringa katkısı, etlik piliçlerde bağışıklık parametrelerini artırken; hastalıkla mücadele de başarılı olmalarını sağlamış; kümes hayvanları için tavsiye edilen bir yem katkısı olmuştur. Denemede Moringa oleifera yaprak ununun karma yeme optimum katılma düzeyinin 4 g/kg olduğu da tespit edildi.

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[1] Khalifa KA., Abedlrahim ES., Elnasri IM., Ahmed SO. A retrospective study (2000- 2005) of poultry diseases diagnosed at department of avian diseases and diagnosis, Veterinary Research Institute (VRI)-Khartoum. Sudanese J of Anim Sci 2013; 9: 42-45.

[2] Raza A, Muhammad F, Bashir S, Anwar MI, Awais MM, Akhtar M., Naseer MU. Antiviral and immune boosting activities of different medicinal plants against Newcastle disease virus in poultry, World’s Poultry Science Journal 2015; 71(3): 523-532.

[3] Fahey JW. Moringa Pleifera: A review of the medical evidence for its nutritional, therapeutic and prophylactic. Properties, part 1. Trees for Life Journal 2005; 1-5.

[4] Aruna M., Srilatha N. Water clarification using Moringa oleifera Lam. seed as a natural coagulant, Current Biotica 2012; 5(4): 472-486.

[5] Ikubanni PP., Komolafe OO, Agboola OO., Osueke CO. Moringa seed dehulling machine: a new conceptual design. Journal of Production Engineering 2017; 20(2): 73-78.

[6] Yameogo C., Bengaly M., Savadogo A., Nikiema P., Traore S. Determination of chemical composition and nutritional values Moringa oleifera leaves, Pak J Nutr 2011; 10: 264-268.

[7] Ezejindu DN, Akiingboye AJ. Protective effects of Moringa oleifera leaf extract on murcury induced spleenotoxity in adult wistar rats, Int Mult-dis e- J 2014; 3: 57- 63.

[8] Perez A., Sánchez T., Armengol N., Reyes F. Characteristics and potential of Moringa oleifera, Lamark. An alternative for animal feeding. Price, M.L. 2007. The Moringa Tree. Echo Technical Note.

[9] Orwa C., Mutua A., Kindt R., Jamnadass R., Anthony S. Agro-forestry Database: a tree reference and selection guide version 4.0. World Agro-forestry Centre, Kenya. 2009; 335-336.

[10] Gaia S. Wonder tree 100 facts Moringa fact 04 exceptional animal feed Moringa as livestock feed & pet food, Moringa Mission Trust. 2005.

[11] Khalafalla MM., Abdellatef E., Dafalla HM., Nassrallah AA., Aboul-Enein KM., Lightfoot DA. Active principle from Moringa oleif.era Lam Leaves effective against two leukemias and a hepatocarcinoma, African Journal of Biotechnology 2010; 9: 8467-8471.

[12] Anamika G., Manish K., Rahul K., Rao C., Goel R., Shampa A. Immunumodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice, Indian Journal of Experimental Biology 2010; 48: 1157-1160.

[13] Fuglier LJ. The miracle tree: Moringa oleifera. Natural nutrition for the tropics. Church World Service, Dakkar, Senegal, 1999; 68.

[14] Feustel S., Ayon-Perez F., SandovalRodriguez A., Rodriguez-Echevarria R., Contreras-salinas H., Armendarz-Borunda J., Sanchez-Orozco LV. Protective effects of moringa oleifera on hbv genotypes c and h transiently transfected huh7 cells, Journal of Immunology Research, 2017.

[15] Teixeira EMB., Carvalho MRB., Neves VA., Silva MA., Arantes-Pereira L. Chemical characteristics and fractionation of proteins from Moringa oleifera lam. leaves, Food Chemistry 2014; 147: 51-54.

[16] Gakuya DW., Mbugua PN., Kavoi B., Kiama SG. Effect of supplementation of Moringa oleifera leaf meal in broiler chicken feed, International Journal of Poultry Science 2014; 13: 208-213.

[17] Nouman W., Basra SMA., Siddiqui MT., Yasmeen A., Gull T., Alcayde MAC. Potential of Moringa oleifera L. as livestock fodder crop: a review, Turkish Journal of Agriculture and Forestry 2013; 37: 1-1.

[18] Ayssiwede S., Dieng A., Bello H., Chrysostome CAAM., Hane MB., Mankor A., Missohou A. Effects of Moringa oleifera (Lam.) leaves meal incorporation in diets on growth performances, carcass characteristics and economics results of growing indigenous Senegal chicken, Pakistan Journal of Nutrition 2011; 10(12): 1132-1145.

[19] Yan Z., Du Y., Zhao Q., Fan R., Guo W., Ma R., Wang X., Zhu R. Mucosal immune responses against live newcastle disease vaccine in immunosuppressed chickens, Pak Vet J 2011; 31: 280-286.

[20] Benjamin MH. Outline of Veterinary Clinical Pathology. 3rd ed., Iowa State University Press, Ames, U.S.A, 1978; 38-40.

[21] Minitab INC. Minitab 16 statistical software. URL:[Computer software]. State College, PA: Minitab, Inc. http://www.minitab.com/en-us/, 2010.

[22] Mahmood S., Rehman A., Yousaf M., Akhtar P., Abbas G., Hayat K., Shahzad MK. Comparative efficacy of different herbal plant’s leaf extract on haematology, intestinal histomorphology and nutrient digestibility in broilers, Adv Zool Bot 2015; 3(2): 11-16.

[23] Gadzirayi CT., Masamha B., Mupangwa JF., Washaya S. Performance of broiler chickens fed on mature Moringa oleifera leaf meal as a protein supplement to soyabean meal, Inter J of P Sci 2012; 11(1): 5-10.

[24] Melesse A., Tiruneh W., Negesse T. Effects of feeding Moringa stenopetala leaf meal on nutrient intake and growth performance of Rhode Island Red chicks under tropical climate, Trop Subtrop Agroeco 2011; 14: 485–492.

[25] Banjo OS. Growth and performance as affected by inclusion of Moringa oleifera leaf meal in broiler chicks diet, J. of Bio Agri Healthcare 2012; 2: 35-39.

[26] Mahmood S., Hayat K., Yousaf M., Abbas G., Rehman A. Comparative effect of Azadirachta indica, Moringa oleifera and Cichorium intybus leaf extracts on growth performance, immune response and carcass characteristics of broilers, Scholar’s Adv. Anim. Vet. Res 2016; 3: 60-65.

[27] Balami AG., Abdu PA., Wakawa AM., Aluwong T. The effect of very virulent infectious bursal disease virus on immune organs of broilers fed moringa oleifera supplemented feed, World 2016; 6(3): 95-100.