Edible species of mushrooms, which grow naturally and have important ecological tasks, are collected and consumed as a foodstuff in our country as well as in many regions of the world. Many studies to determine the chemical structure of the mushrooms have revealed that they are valuable nutrient sources in terms of essential fatty acids, essential amino acids, vitamins, and minerals. It is especially preferred by people who are dieting because they are poor in terms of calories and fat. This study aimed to determine the fatty acid contents of Cyclocybe aegerita (V. Brig.) Vizzini and Hygrophorus eburneus (Bull.) Fr. Mushroom samples collected from the natural environment. Mushroom samples, which are the material of our study, were collected in Tokat during field trips. The analyzes were performed by GC-MS instrument after obtaining methyl esters of fatty acids. At the end of the studies, palmitic acid C16:0, stearic acid C18:0, oleic acid C18:1(n-9) and linoleic acid C18:2 (n-9-12) from C. aegerita samples; myristic acid C14:0, palmitic acid C16:0, palmitoleic acid C16:1 (n-9), stearic acid C18:0, oleic acid C18:1(n-9), linoleic acid C18:2 (n-9-12), and nervonic acid C24:1(n-9) from H. eburneus samples were determined in different proportions. The highest amount of fatty acid was found linoleic acid in C. aegerita with proportions of 54.33% and oleic acid in H. eburneus samples with proportions of 47.80%. In addition to also in both mushroom species, the total saturated fatty acid rate was higher than the total unsaturated fatty acid rate.
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