İnsuyu Mağarası’ndan İzole Edilen Bakterilerin Antibiyotik Dirençliliğinin Araştırılması

Ekstrem çevrelerde farklı metabolik özellikteki mikroorganizmaların keşfi için yapılan çalışmalar her geçen yıl artmaktadır. Bu çalışma kapsamında ekstrem çevre olarak İnsuyu mağarasından örneklemeler yapılmıştır. Elde edilen izolatlarda antibiyotik dirençliliği kültürel ve moleküler biyoloji yöntemleri ile araştırılmıştır. Çoklu direnç gösteren 10 izolat ve duyarlı 3 izolat seçilerek 16S rRNA dizi analizi ile tür tayini yapılmıştır. Çoklu direnç gösteren Pseudomonas spp. (2G-2), Pseudomonas jesseni (2J), Sphingopyxis fribergensis (4D), Microbacterium yannicii (4M), Flavobacterium chungangense (6B), Rhodococcus spp. (7A), Flavobacterium resistens (9D), Pseudomonas spp. (K-4F), Pseudomonas spp. (K-4G), Buttiauxella agrestis (K-15A) ve duyarlılık gösteren Flavobacterium chungangense (K-1E), Rhodococcus erythropolis (K-11G) ve Pseudomonas spp. (K-15G) suşlarında antibiyotik direnç genleri taranmıştır. Pseudomonas jesseni (2J) ve Pseudomonas spp. (K-4G)’ de aminoglikozit 2’’-O-nükleotiltransferaz (aadB), beta laktamaz (blaCTXM3, blaSHV, blaTEM), aminoglikozit direnç proteini (strA), rifampin ADP- ribosiltransferaz (arr2- int2a), vankomisin (vanC) olmak üzere 8 farklı gen bölgesi saptanmıştır.

Anahtar Kelimeler:

Antibiyotik direnci, ekstrem çevreler, çoklu ilaç direnci

Investigation of Antibiotic Resistance of Bacteria Isolated from Insuyu Cave, Burdur-Turkey

Studies for the discovery of microorganisms with different metabolic properties in extreme environments are increasing every year. Within the scope of this study, samples were taken from, an extreme environment, İnsuyu cave. Antibiotic resistance in the obtained isolates was investigated by cultural and molecular biology methods. As a result of Minimum Inhibition Concentration (MIC) test, 64 cefpodoxim, 6 rifampicin and gentamycin, 18 vancomycin, 15 ampicillin, 44 clindamycin, 48 penicillin resistant strains were detected. Pseudomonas spp. (2G-2), Pseudomonas jesseni (2J), Sphingopyxis fribergensis (4D), Microbacterium yannicii (4M), Flavobacterium chungangense (6B), Rhodococcus spp. (7A), Flavobacterium resistens (9D), Pseudomonas spp. (K-4F), Pseudomonas spp. (K-4G), Buttiauxella agrestis (K-15A) were found to have multidrug resistant by cultural methods. These bacteria and susceptible strains, Flavobacterium chungangense (K-1E), Rhodococcus erythropolis (K-11G) and Pseudomonas spp. (K-15G), as negative controls were selected for the identification of antibiotic resistance genes by PCR based methods. Eight different gene regions, aminoglycoside 2''-O-nucleotyltransferase (aadB), beta lactamase (blaCTXM3, blaSHV, blaTEM), aminoglycoside resistance protein (strA), rifampin ADP- ribosyltransferase (arr2- int2a), vancomycin (vanC) have been detected in Pseudomonas jesseni (2J) and Pseudomonas spp. (K-4G).

Keywords:

Antibiotic resistance, extreme environment, multidrug resistance,

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