Yaprakbitlerinden elde edilen bazı fakültatif endosimbiyont türlerin moleküler karşılaştırılması

Yaprakbitleri tarımsal alanlarda pek çok konukçu bitkide yaptığı zarardan dolayı önemlidir. Yaprakbitlerinin konukçu bitki seçimi, üreme, ilaçlara dayanıklılık gibi biyolojik aktivitelerinde anahtar öneme sahip endosimbiyotlar mevcuttur. Zararlının biyolojisini bu kadar etkileyen endosimbiyontlar üzerine ülkemizde pek çalışma yoktur. Bu nedenle yürütülen bu çalışmada da Adana ilinde farklı lokasyon ve bitkiler üzerinde toplanan yaprakbiti türlerindeki endosimbiyontlar üniversal bir primer ile tespit edilmiştir. Elde edilen endosimbiyontların filogenetik ilişkisi Maximum Likelihood metoduna göre ortaya konulmuştur. Yapılan analizler sonucunda yaprakbiti türleri üzerinden Buchnera aphidicola, Arsenophonus, Candidatus Hamiltonella defensa, Ca. Fukatsuia symbiotica, Ca. Serratia symbiotica endosimbiyont türleri elde edilmiştir. Filogenetik ağaç üzerinde tüm endosimbiyont türler yüksek Bootstrap değerleriyle birbirlerinden ayrılmıştır. Aphis craccivora’nın Robinia pseudoacacia’ya özgü haplotip geliştirmesinde etken olduğu düşünülen fakültatif Arsenophonus bu çalışmada da belirlenmiştir.

Molecular comparison of some facultative endosymbiotic species obtained from aphids

Aphids are important in agricultural areas due to the damage they do to many host plants. The endosymbionts play a key role in the biological activities of aphids such as host plant selection, reproduction, and drug resistance. Very few studies on endosymbionts that affect the biology of the pest so much were conducted in our country. In this study, endosymbionts in aphid species collected on different locations and plants in Adana province were determined with a universal primer. The phylogenetic relationship among obtained endosymbionts was determined according to the Maximum Likelihood method. As a result of the analysis, Buchnera aphidicola, Arsenophonus, Candidatus Hamiltonella defense, Ca. Fukatsuia symbiotica, Ca. Serratia symbiotica, endosymbiont species obtained on the different aphid populations. All endosymbiont species on the phylogenetic tree were separated from each other by high Bootstrap values. The facultative Arsenophonus, which is thought to be a factor in the development of specific haplotype to Aphis craccivora on Robinia pseudoacacia was also identified in this study.

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Mediterranean Agricultural Sciences-Cover
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1988
  • Yayıncı: Akdeniz Üniversitesi
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