Türkiye’de yetiştirilen beş sığır ırkında MYF5 gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi

Bu çalışmada Türkiye’de yetiştirilen Simental (SİM, n= 75), Holstein (HL, n=100), İsviçre Esmeri (ES, n=75), Doğu Anadolu Kırmızısı (DAK, n= 40) ve Boz Irk (BI, n=40) sığırlarının MYF5 gen polimorfizminin restiriksiyon parçacık uzunluk polimorfizmi (RFLP) metoduyla belirlenmesi amaçlanmıştır. MYF5 allelle ve genotipleri elde edilen polimeraz zincir reaksiyonu (PCR) ürünlerine TaqI kesim enzimi ile muamele edilmesiyle belirlenmiştir. Çalışmada incelenen ırklar içinde en yüksek AA genotip frekansı DAK (0.20), en yüksek GG genotip frekansı BI (0.53) ve en yüksek AG genotip frekansı ise ES (0.53) ırkında görülmüştür. MYF5 geni yönünden incelenen ırklar arasında en yüksek A allel frekansı DAK ırkında (0.43), en yüksek G allelinin frekansı ise BI’ta (0.73) bulunmuştur. Çalışma sonunda incelenen beş sığır ırkının da MYF5 geni yönünden Hardy-Weinberg dengesinde olduğu görülmüştür.

Anahtar Kelimeler:

Sığır, Genetik belirteç, MYF5, Polimorfizm

Detection of MYF5 gene polymorphism with PCR-RFLP method in five cattle breeds breeding in Turkey

The aim of this study was to detection of the MYF5 gene polymorphism in Simmental (SIM, n=75), Holstein (HL, n=100), Brown Swiss (ES, n=75), East Anatolian Red (EAR, n=40) and Turkish Grey (TG, n=40) cattle breeds raised in Turkey through restriction fragment length polymorphism (RFLP) method. The MYF5 alleles and genotypes were detected by digesting the polymerase chain reaction (PCR) products with TaqI endonuclease enzyme. Among the breeds examined for MYF5 gene, the highest AA genotype frequency was found in the EAR cattle breed (0.20); the highest GG genotype frequency was found in the TG cattle breed (0.53) and the highest AG genotype frequency was found in the ES cattle breed (0.53). Among the breeds examined, the highest A allele frequency was found in the EAR cattle breed (0.43) whereas the highest G allele frequency was found in the TG cattle breed (0.73). At the end of the study, it was found that examined five cattle breeds were in Hardy-Weinberg equilibrium (HWE) in terms of MYF5 gene.

Keywords:

Cattle, Genetic marker, MYF5, Polymorphism,

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