T. KARSLI, E. ŞAHİN, B. Argun KARSLI, S. ALKAN, M. S. BALCIOĞLU

Türkiye’de yetiştirilen bazı yerel koyun ırklarında BMP-15 genindeki (FecXG, FecXI, FecXH, FecXB) mutasyonların araştırılması

Koyunlarda majör genler üzerindeki genetik mutasyonlar ovulasyon oranını ve bir batında doğan yavru sayısını artırmaktadır. Şu ana kadar TGF-β süper familyasına ait üç majör gen tanımlanmıştır. Bu genler BMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein-15) ve GDF9 (Growth differentiation factor 9) dur. Koyunlarda Bone Morphogenetic Protein-15 geni üzerindeki farklı mutasyonlar (FecXB, FecXG, FecXI, FecXH, FecXL ve FecXR) ovulasyon oranını artırmakta ya da kısırlığa neden olmaktadır. Bu çalışmanın amacı Türkiye’de yetiştirilen beş yerel koyun ırkında BMP-15 geninde dört farklı mutasyonun (FecXG, FecXI, FecXH, FecXB) olup olmadığının araştırılmasıdır. Bu mutasyonları belirlemek için PCR-RFLP yöntemi kullanılmıştır. Akkaraman (24 örnek), Dağlıç (19 örnek), İvesi (19 örnek), Tuj (15 örnek) ve Karakaş (19 örnek) koyun ırklarından toplam 96 kan örneği incelenmiştir. FecXG, FecXI, FecXHve FecXB mutasyonlarının belirlenmesi için sırasıyla 141, 154, 240 ve 153 bç büyüklüğündeki DNA fragmentleri spesifik primerler kullanılarak PCR ile çoğaltılmıştır. PCR ürünleri, FecXG, FecXI, FecXH, FecXB nokta mutasyonlarını belirlemek için sırasıyla HinfI, XbaI, AhlI, BstDEI restriksiyon enzimleri kullanılarak kesilmiştir. Sonuç olarak incelenen örneklerde FecXI, FecXH, FecXGve FecXB lokusları tespit edilememiştir. Beş sürüden rasgele seçilen 96 koyun FecXI, FecXH, FecXGve FecXB lokusları bakımından monomorf bulunmuştur.

Anahtar Kelimeler:

BMP-15 geni, Çoklu doğum, Koyun

An investigation of mutations (FecXG , FecXI , FecXH , FecXB ) on BMP-15 gene in some local sheep breeds raised in Turkey

Genetic mutations on major genes increase ovulation rate and litter size in sheep. Three major genes have been identified belonging to the TGF-β superfamily until now. These genes areBMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein15) and GDF9 (Growth differentiation factor 9). Different mutations (FecXB, FecXG, FecXI,FecXH, FecXL and FecXR) on Bone Morphogenetic Protein-15 gene either increase ovulation rate or cause infertility in sheeps. The purpose of this study was to investigate whether the four different mutations (FecXG, FecXI, FecXH and FecXB) exist on BMP15 gene in five local sheep breeds reared in Turkey. PCR-RFLP method was used to determine these mutations. A total of 96 blood samples were investigated from Akkaraman (24 samples), Dağlıç (19 samples), İvesi (19 samples), Tuj (15 samples) and Karakaş (19 samples) sheep breeds. To determine FecXG, FecXI, FecXH and FecXB mutations, DNA fragments with 141, 154, 240 and 153 bp sizes were PCR amplified by using spesific primers, respectively. PCR products were digested to detect FecXG, FecXI, FecXH, FecXB point mutation by using HinfI, XbaI, AhlI, BstDEI restriction enzymes, respectively. As a result, FecXI, FecXH, FecXG and FecXB loci could not bedetermined. Random selected 96 sheeps from five flocks were monomorph in terms of FecXI, FecXH, FecXG and FecXB loci.

Keywords:

BMP-15 gene, Prolificacy, Sheep,

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