A. KHAR, R. C. YADAV, N. YADAV, R. D. BHUTANI

Soğan (Allium cepa L.) ve Sarmısakda (Allium sativum L.) Transient Gus Expression Çalışmaları

Bu çalışmada, transient gus ekpressiyonu amacıyla, 3 soğan (B-780, N-2-4-1 ve Hisar-2) ve 2 sarmısak (HG1, G-41 and G-1) genotipi kullanılmıştır. Bu amaçla, soğanda kallus, sürgün ucu ve kök; sarmısakta ise kök, basal kısım ve genç etiyolleşmiş yapraklar kullanılarak 2 farklı plazmidin (pTOK 233 ve pCAMBIA 1301) transformasyon için uygunluğu araştırılmıştır. Soğanda eksplant kaynağı olarak kallusun kullanımı sürgün ucuna göre daha başarılı bulunmuş ve kök uçlarında gus epressionu gözlenmemiştir. Sarmısakta, sadece basal kısımda transient ekpression belirlenmiş, yaprak ve kökler her hangi bir yanıt göstermemişlerdir. Genotipler arasında gus transient ekspression bakımından bir farklılık belirlenmemiş, fakat plasmidler arasında farklılık kaydedilmiştir. Plasmid pCAMBIA 1301, pTOK 233’e göre önemli bir üstünlük göstermiştir

Anahtar Kelimeler:

Transformasyon, gus ekspression, Allium cepa, Allium sativum

Transient Gus Expression Studies in Onion (Allium cepa L.) and Garlic (Allium sativum L.)

Three genotypes of onion B-780, N-2-4-1, Hisar 2 and garlic HG-1, G-41 and G-1 were used to study the transient gus expression. Also different explants in onion, callus, shoot tip and roots and in garlic, roots, basal portion and young etiolated leaves along with two plasmids were used to study the effect of different plasmids (pTOK 233 and pCAMBIA 1301) and suitability of explants towards transformation. Results revealed that in onion callus obtained from different explant sources performed significantly superior than shoot tip whereas in root tip no gus expression was observed. In garlic, only basal portion showed transient expression whereas leaves and roots did not show any response. There were no significant differences among genotypes but plasmids showed significant differences. Genotypes differences were not significant; however, the two plasmids showed variables response transient gus assays. Plasmid pCAMBIA 1301 show significant superiority over pTOK 233 in these experiments.

Keywords:

Transformation, gus expression, Allium cepa, Allium sativum,

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