Salvia candidissima Vahl.’ın uçucu yağ ve ekstraktının kimyasal bileşenlerinin, antioksidan ve antifungal aktivitesinin belirlenmesi

Bu çalışmanın amacı, ada çayı (Salvia candidissima Vahl.) bitki uçucu yağının bileşenlerinin ve bitki patojeni fungal etmenlere karşı antifungal etkinlikleri, bitki metanolik ekstraktının ise antioksidan aktivitelerinin araştırılmasıdır. S. candidissima uçucu yağında GC/MS kullanılarak 54 bileşen belirlenmiştir. Tanımlanan bu bileşenler uçucu yağın % 99.98’ini oluşturduğu saptanmıştır. Uçucu yağın temel bileşenleri Spathulenol (% 12,75), Caryophyllene oxide (% 8.67), Ledene oxide (% 6.98) ve o-Cymene (% 6.03) olarak belirlenmiştir. Uçucu yağın, antifungal etkinliği fungal hastalık etmenlerinden Rhizoctonia solani ve Aternaria solani’ye karşı araştırılmıştır. Çalışma sonucunda 10 μl petri-1 uçucu yağ uygulaması sonucunda A. solani’nin miselyum gelişimini % 57.92 oranında R. solani’nin miselyum gelişimini ise % 51.87 oranında engellediği belirlenmiştir. Bitki ekstraktları ile yapılan antioksidan çalışması sonucunda, Serbest Radikal Giderme DPPH (lC50 22.96 ± 0.45), Demir İndirgeme Gücü (FRAP 1.20 ± 0.16 mmol TE g-1 eksrakt), Bakır İndirgeme Gücü (CUPRAC 3.30 ± 0.12mmol TE g-1 ekstrakt) ve Serbest Katyon Radikali Giderme TEAC ise (lC50 9.25 ± 0.40 (µg ml-1)) olarak belirlenmiştir. Toplam Fenolik (TP) ve Toplam Flavonoid (TF) içeriği sırasıyla 83.53 ± 5.92 mg GAE g-1 ekstrakt ve 59.02 ± 3.59 mg QE g-1 ekstrakt olarak tespit edilmiştir. Bu sonuçlar; S. candidissima uçucu yağın bitki patojeni funguslara karşı dikkate değer bir antifungal aktiviteye sahip olmasının yanısıra bitki metanolik ekstraktları güçlü bir antioksidant kapasitesine sahip olduğunuda göstermiştir.

Anahtar Kelimeler:

Antioksidant, Antifungal, Uçucu yağ, Kimyasal bileşen, Salvia candidissima Vahl.

Determination of the chemical components, antioxidant and antifungal activities of essential oil and plant extract of Salvia candidissima Vahl.

The aim of this study is to investigate the chemical components, antioxidant and antifungal activities of the essential oil and plant methanolic extract of Salvia candidissima Vahl. plant, which is one of the sage species. By using Gas Chromatography (GC) and Gas Chromatography Mass Spectrometry (GC-MS) method, it was determined that there are 54 components in the essential oil of S. candidissima. These identified components were found to constitute 99.98% of the essential oil. The main components of the essential oil were determined as Spathulenol (12.75%), Caryophyllene oxide (8.67%), Ledene oxide (6.98%) and o-Cymene (6.03%). A antifungal activity of the essential oil against Rhizoctonia solani and Aternaria solani was found. As a result of the study, it was determined that the mycelial growth of A. solani was inhibited by 57.92% as a result of application of 10 μL petri-1 essential oil, while that of R. solani was inhibited by 51.87%. As a result of the antioxidant study conducted with plant extract, the values of Free Radical Scavenging DPPH (lC50 22.96 ± 0.45), Iron Reduction Power (FRAP 1.20 ± 0.16 mmol TE g-1 extract), Copper Reduction Power (CUPRAC 3.30 ± 0.12 mmol TE g-1 extract) and Free Cation Radical Scavenging TEAC (lC50 9.25 ± 0.40 (µg ml-1)) were determined. The Total Phenolic (TP) and Total Flavonoid (TF) contents were found as 83.53 ± 5.92 mg GAE g-1 extract and 59.02 ± 3.59 mg QE g-1 extract, respectively. These results showed that the essential oil and plant methanolic extract of S.candidissima has a significant antifungal activity against plant pathogenic fungi and strong antioxidant activity.

Keywords:

Antioxidant, Antifungal, Essential oil, Chemical component, Salvia candidissima Vahl.,

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Bayrak A, Akgül A (1987) Composition of essential oils from Turkish Salvia species. Phytochemistry; 26: 846–847.
Chalchat JC, Michet A, Pasquier B (1998) Study of the clones of Salvia officinalis L. yields and chemical composition of essential oil. Flavour And Fragrance Journal, Vol.13, 68-70.
Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods”. J. Food Drug Analysis, 10, 178-182.
Çadırcı E, Süleyman H, Gürbüz P, Kuruüzüm UA, Güvenalp Z, Demirezer LÖ (2012) Anti-inflammatory effects of different extracts from three Salvia species. Turk. J. Biol., 36: 59-64.
Delamare APL, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S (2007) Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem., 100 pp. 603–608.
Demirci B, Başer KHC, Yıldız B, Bahçecioğlu Z (2003) Composition of the essential oils of six endemic Salvia spp. from Turkey. Flavour Fragr. J., 18, 116–121.
Diri HA (2006) Salvia candidissima VAHL. Characterization Of Essential Compositions and Determınation Of Antioxidant Activity of Salvia candidissima Vahl. MUGLA University Institute of Science and Technology. pp. 104.
Halliwell B, Gutteridge JMC (1990) A Role of free radicals and catalytic metal ions in human disease: An overview. In: Methods in Enzymology, 186, 1-85.
Karabacak E (2009) Türkiye'nin Avrupa-Sibirya fitocoğrafik bölgesindeki Salvia L. (Lamiaceae) cinsinin revizyonu. (Doktora Tezi). Çanakkale Onsekiz Mart Üniversitesi. Fen Bilimleri Enstitüsü, Çanakkale, s. 205.
Karık M (2013) Marmara Bölgesindeki Anadolu Ada Çayı (Salvia fruticosa Mill.) Populasyonlarının Morfolojik ve Kalite Özelliklerinin Belirlenmesi, Kültüre Alınma Olanaklarının Araştırılması. Doktora tezi T.C Namık Kemal Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Ana Bilim Dalı. 2013, s. 4-5.
Kelen M, Tepe B (2008) Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Biores. Technol., 99, pp. 4096–4104.
Kılıç T, Dirmenci T, Satil F, Bilsel G, Kocagoz T, Altun M, Goren AC (2005) Fatty acid composition of seed oils of three Turkish Salvia species and biological activities. Chemistry of Natural Compounds, 41, 276-279.
Liyana-Pathirana CM, Shahidi F (2005). Antioxidant activity of soft commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions”. J. Agric. Food Chem; 53: 2433–2440.
Oyaizu M (1986) Studies on product of browning reaction prepared from glucose amine. Japan Journal of Nutrition, 44, 307-315.
Özler MA, Duru ME, Diri HA, Harmandar M (2009) Antioxıdant Activity And Chemical Composition of The Essential oil of Salvia Candidissima Vahl. Grownig Wild in Turkey. I International Medicinal and Aromatic Plants Conference on Culinary Herbs.
Pandey DK, Tripathi NN, Tripathi RD, Dixit SN (1982) Fungitoxic and phytotoxic properties of essential oil of Hyptis suaveolens. Z Pflanzenkrankheiten Pflanzenschutz 89: 344–349.
Re R, Pellegrini N, Protrggente, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Madicine, 26: 1231-1237.
Serteser A, Gök V (2003) Doğal Antioksidanların Biyoyararlılığı. 3. Gıda Mühendisliği Kongresi. 2-4 Ekim, S. 83-98, Ankara.
Seyoum A, Asres K, El-Fiky FK (2006) Structure-radical scaveng-ing activity relationship of flavonoids. Phytochemistry. 67: 2058-2070.
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folinciocalteu reagent”. Methods in Enzymology, 299: 152-178.
Şenkal BC, İpek A, Gürbüz B (2012) Türkiye florasında bulunan adaçayı (Salvia L. spp.) türlerinin uçucu yağ içeriklerinin değerlendirilmesi. Tıbbı ve Aromatik Bitkiler Sempozyumu 13-15 Eylül 2012, s. 166-176.
Telci I, Bayram E, Yilmaz G, Avci B (2006) Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochemical Systematics and Ecology, 34, 489-497.
Tepe B, Sokmen M, Akpulat HA, Sokmen A (2006) Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chemistry 95 200–204.
Tosun M, S Ercisli, Sengul M, Ozer H, Polat T, Ozturk E (2009) Antioxidant Properties and Total Phenolic Content of Eight Salvia Species from Turkey. Biol Res 42: 175-181.
Yılar M (2014) Tokat Ve Çevresinde Yaygın Olarak Görülen Salvıa Türlerinin Antifungal Ve Biyoherbisidal Aktivitelerinin Belirlenmesi, Doktora Tezi T.C Gaziosmanpaşa Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı. s. 58.
Yılar M, Kadıoglu I (2016) Antifungal Activities of some Salvia Species Extracts on Fusarium oxysporum f. sp. radicis- lycopersici (Forl) Mycelium Growth In-vitro. Egyptian Journal of Biological Pest Control, 26(1), 115-118.
Yıldırım N, Bekler FM, Yıldırım NC (2010) In Vıtro Antimicrobial Evaluation of Commercial Tea Extracts Against Some Pathogen Fungi and Bacteria. Dig. J. Nanomat. Biostruc. 5 (4), 821-827.
Young IS, Woodside J V (2001) Antioxidants in Health and Disease”. Journal of Clinical Pathology, 54: 176-186.