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Markör destekli piramit potivirüs direnç genleri, Ruanda fasulye (Phaseolus vulgaris L.) genotipleri

Özellikle Doğu ve Orta Afrika'da bulunan ve fasulye veriminde önemli bir tehdit olan fasulye adi mozaik virüsü ve fasulye adi mozaik nekroz virüsü potirviruse aittir. Dayanıklılığı arttırmak ve verimi stabilize etmek için dominant ve resesif direnç genlerini birleştirmek virüsün bilinen tüm suşlarına karşı en etkili hastalık kontrol stratejisidir. Çalışmanın amacı, hem dominant I hem de resesif bc-3 genlerini, marker destekli geri melezleme ıslahı yoluyla hassas Ruanda fasulyelerine transfer etmektir. I geni (BCMV-48289723-CAPS) ve bc-3 geni (ENM-CAPS) için spesifik markerler BC progenlerinde seçilim için kullanıldı. Başarılı gen kombinasyonu progenilerde BC1F1 ve BC2F1'in %33'ünde, BC3F1'in %43'ünde ve BC3F2 %16'sında başarıyla gerçekleştirilmiştir. Tohum rengi, bazı BC3 progenilerinde kombine direnç genleriyle tamamen geri kazanılmıştır. Gelişmiş hatların, mahsulü Doğu ve Orta Afrika koşullarında hem BCMV hem de BCMNV'ye karşı koruması beklenmektedir.

Anahtar Kelimeler:

Geri melezleme, Fasulye adi mozaik virüsü, Fasulye adi mozaik nekroz virüsü, Fasulye

Marker-assisted pyramiding potyvirus resistance genes into Rwandan common bean (Phaseolus vulgaris L.) genotypes

Bean common mosaic virus and Bean common mosaic necrosis virus belong to Potyvirus and the disease poses significant threat to bean yield especially in East and Central Africa. Combining the dominant and recessive resistance genes is the most effective disease control against all known strains of the virus, a strategy to enhance the durability and stabilize the yield. The aim of the study was to transfer both the dominant I and recessive bc-3 genes into susceptible Rwandan common beans via marker-assisted backcross breeding. The markers specific for I gene (BCMV-48289723-CAPS) and for bc-3 gene (ENM-CAPS) were used for selection in BC progenies. Successful gene combination was established in 33% of BC1F1 and BC2F1, 43% of BC3F1 and 16% of BC3F2 progenies. The seed color was fully recovered on some of the BC3 progenies with combined resistance genes. The advanced lines are expected to shield the crop against both BCMV and BCMNV under East and Central African conditions.

Keywords:

Backcross (BC), Bean common mosaic virus (BCMV), Bean common mosaic necrosis virüs (BCMNV), Phaseolus vulgaris,

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