Çanakkale ilinde Karnabahar mozaik virüsü (Cauliflower mosaic virus; CaMV) izolatlarının tanılanması ve karakterizasyonu

Bu çalışmada Çanakkale ili ve ilçelerinde 2010-2011 üretim yılları içinde bir sörvey çalışması yürütülerek Karnabahar mozaik virüsü (Cauliflower mosaic virus, CaMV)’ne benzer semptom gösteren karnabahar ve lahana bitkilerinden 84 örnek toplanmıştır. Toplanan örnekler CaMV’nin varlığını belirlemek amacıyla DAS-ELISA yöntemiyle test edilmiştir. DAS-ELISA testi sonucunda 84 örnekten 63’ü CaMV ile infekteli bulunmuştur. İnfekteli bulunan örnekler içerisinden seçilen 6 izolatın moleküler özelliklerini belirlemek amacıyla kılıf protein (CP) genleri klonlanarak nükleik asit dizilimleri belirlenmiştir. Klonlama ve sekans analizi yapılan CaMV izolatlarına özgü nükleotid dizilimleri gen bankasında bulunan ve dünyanın farklı üretim bölgelerinden CaMV izolatlarının kılıf protein genleriyle karşılaştırılmıştır. Bu karşılaştırmalar sonucunda Çanakkale izolatlarının CP nükleotid dizilimlerinin kendi içinde % 93-100, dünya izolatları ile % 92-97 oranında bir benzerliğe sahip olduğu bulunmuştur. Yapılan filogenetik analizler sonucunda Çanakkale izolatlarının dünyanın farklı bölgelerindeki izolatlarla farklı düzeylerde ilişki gösterdiği saptanmıştır.

Anahtar Kelimeler:

Çanakkale, Karnabahar mozaik virüsü, DAS-ELISA, klonlama, sekanslama

Identification and characterization of Cauliflower mosaic virus (CaMV) isolates in Çanakkale province

In this study, a survey was conducted in Çanakkale province and sub provinces on cauliflower and cabbage plants that show Cauliflower mosaic virus-like symptoms between the years 2010-2011. A total of 84 samples were collected from these areas and tested by DAS-ELISA for the presence of Cauliflower mosaic virus (CaMV). As a result of the DAS-ELISA analysis 63 out of 84 samples were found infected with CaMV. The coat protein genes of 6 isolates were cloned and sequenced. Isolates were further characterized and the sequences obtained from CaMV isolates of Çanakkale were compared with known sequences from other part of the world to determine the genetic differences and evolutionary relationships among CaMV isolates from Çanakkale and the other parts of the world. Comparison of CP genes revealed that CP gene of CaMV isolates from Çanakkale province and sub provinces showed 93-100 % and isolates with the world 92-97 % identity in their nucleotide sequence, respectively. Phylogenetic analysis of the CP gene sequences showed that CaMV isolates from Çanakkale province and sub provinces displayed different level of genetic relationship with CaMV isolates from those of the world.

Keywords:

Çanakkale, Cauliflower mosaic virus, DAS-ELISA, Cloning, sequencing,

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