Bazı maddelerin Heterorhabditis bacteriophora HBH ırkının in vitro kitle üretimi üzerine etkileri

Biyolojik mücadele, tarımda kimyasal pestisitlere karşı önemli bir alternatif yöntemdir. Entomopatojen nematodlar (EPN) çoğunlukla toprak kaynaklı zararlı böceklere karşı kullanılan doğa dostu biyolojik mücadele ajanlarıdır. Uzun yıllardan beri EPN’ler kitle halinde ticari olarak üretilmektedirler. Kitle üretimi genellik in vitro katı ve sıvı kültürler içerisinde yapılmaktadır. Kitle üretiminden sonra EPN’ler çeşitli dolgu maddeleri ile formüle edilmekte ve ticari ürün olarak satılmaktadır. Kitle üretiminde kullanılan en yaygın maddeler arasında protein ve yağ kaynağı olarak Nutrient Broth, Yeast ekstraktı, soya unu ve çeşitli bitkisel yağlar bulunmaktadır. Kitle üretiminin verimini artırmak için bu üretim ortamı içerisinde farklı maddeler eklenebilmektedir. Bu çalışmada, lesitin ve yumurta sarısı ve ikisinin birleşiminin Heterorhabditis bacteriophora HBH ırkının kitle üretiminin verimi üzerindeki etkileri incelenmiştir. Çalışmada H. bacteriophora türünün Türkiye’de farklı iklime sahip bölgelere ait izolatlarının hibritlenmesi ile elde edilmiş HBH ırkı kullanılmıştır. Kitle üretiminin veriminin hesaplanmasında infektif jüvenil (IJ) sayısı ölçüt olarak belirlenmiştir. Sonuçlar incelendiğinde tüm uygulamaların kontrole göre istatistiksel olarak daha etkili olduğu, en iyi uygulamanın ise lesitin kullanılan ortam ile elde edildiği belirlenmiştir. Bu sonuç ile lesitinin in vitro katı kitle üretiminde ek madde olarak kullanılabileceği tespit edilmiştir.

Anahtar Kelimeler:

Entomopatojen nematod, in vitro, Katı ortam, Biyolojik mücadele

Effects of several ingredients on in vitro mass production of Heterorhabditis bacteriophora HBH strain

Biological control is a great alternative to chemical pesticides in agriculture. Entomopathogenic nematodes (EPNs) are environmentally safe biocontrol agents that are used mostly against soil-dwelling insect pests. EPNs have been commercially mass produced for several decades. Mass production is generally done in vitro using solid and liquid cultures. After mass production, they are formulated with filler compounds and sold as commercial products. Most common substances for in vitro production are nutrient broth, yeast extract, soyflour and different plant oils for protein and lipid source. To increase the production yield of the mass culture, several compounds can be added to the media. In this study; lecithin, egg yolk and the both together were used in solid medium and effects of these additional compounds were examined on mass production of hybrid Heterorhabditis bacteriophora HBH strain, which was obtained from hybridization of Turkish isolates. Infective juveniles (IJs) of the strain were targeted as mass production yield criteria. According to the results, all treatments showed statistically better yields compared to control, and lecithin was statistically the best treatment among others. Thus, lecithin could be used as an additional compound in in vitro solid mass production media.

Keywords:

Entomopathogenic nematode, in vitro, Solid media, Biological control,

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