The immune causes of non-responsiveness to hepatitis B vaccine in uremics and the changes in the immunity in zinc deficiency have been found to have similarities. The aim of this study was to investigate the role of serum zinc levels on the response to hepatitis B vaccination in hemodialysis children. Hepatitis B serological markers and serum zinc levels were determined in total 33 hemodialysis children. Seronegative 17 patients were vaccinated and evaluated for the response. Serum zinc concentrations was measured by atomic absorption spectrophotometer. Age equivalent 50 healthy children formed the control group. Statistical analysis was perfomed with Mann-Whitney non-parametric test. The mean serum zinc levels of the total hemodialysis group as well as the responder and non-responder subgroups were statistically lower than the control group. No statistical difference in serum zinc levels between the responders and non-responders was found. In conclusion, the poor response to hepatitis B vaccination in hemodialysis children was not found to have any connection with low serum zinc levels.