Spermatozoon liyofilizasyonu: Hayvan genetik kaynaklarının korunması için yeni bir saklama modeli olabilir mi?
Her ülkenin, kendine özgü hayvan genetik kaynaklarını, nesli tükenme tehlikesine karşın veya gelecekte gen aktarım çalışmaları için koruması, önemli bir stratejik planlamadır. Gen bankalarında sıvı azot bağımlı olarak saklanan spermatozoon genetik materyallerinin, sıvı azot ile kontaminasyon riskinin bulunması ve sıvı azot ikmali aksaklıklarına bağlı olarak, spermatozoonların zarar görerek fertilizasyon yeteneğini kaybetmesi muhtemeldir. Son yıllarda sıvı azot ile spermatozoon saklama modeline alternatif olarak gösterilen spermatozoon liyofilizasyonu (dondurup-kurutarak saklama) işlemi sonucunda, spermatozoon motilite kaybı ve hücre membran hasarı görülmesine rağmen DNA bütünlüğü korunmaktadır. Liyofilize spermatozoon genetik materyalinin, intra-sitoplazmik spermatozoon enjeksiyonu (ICSI) ile laboratuvar hayvanları başta olmak üzere yaban hayvanları ve çiftlik hayvanlarında in vitro fertilizasyon uygulaması ile yavru alınması mümkündür. Bu derlemede liyofilize spermatozoon protokolleri, saklama koşulları ve in vitro fertilizasyon çalışmaları hakkında bilgi verilmesi amaçlanmıştır.
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- 1. Abdalla H, Hirabayashi M, Hochi S (2009): The ability of freeze-
dried bull spermatozoa to induce calcium oscillations and
resumption of meiosis. Theriogenology, 71(3): 543–52.
- 2. Adams GDJ, Cook I, Ward KR (2015): The principles of freze-drying.
Cryopreservation and Freeze-Drying Protocols. Ed.:
Wolkers WF, Oldenof H, s:121-143.
- 3. Akın AO (2017): Çiftlik Hayvanları genetik kaynaklarının korunması
ve sürdürülebilir kullanımı küresel stratejilerinin ve
Türkiye örneğinin değerlendirilmesi. Doktora Tezi, Ankara
Üniversitesi Fen Bilimleri Enstitüsü.
- 4. Ak K, Cirit Ü, Sandal Aİ, Arıcı R, Özmen MF (2017): Dondurulmuş
Spermanın Depolanması ve Kullanımında Saha Koşullarında
Yapılan Hatalı Uygulamalar ve Çözüm Önerileri. Turkiye
Klinikleri Reproduction and Artificial Insemination-Special
Topics, 3(1): 44-59.
- 5. Anzalone DA, Palazzese L, Iuso D, Martino G, Loi P (2018):
Freeze-dried spermatozoa: An alternative biobanking option
for endangered species. Animal Reproduction Science, 190:
85–93.
- 6. Arav A, Saragusty J (2016): Directional freezing of sperm
and associated derived technologies. Animal Reproduction
Science, 169: 6–13.
- 7. Benedict M (1905): The determination of water in foods and
physiological preparations. Arn J Physiol, 13: 309–329.
- 8. Bialy G, Smith VR (1957): Freeze-drying of bovine spermatozoa.
Journal of Dairy Science, 40: 739-745.
- 9. Billy D, Potts M (2001): Life and death of dry prokaryotes. Res
Microbiol, 153: 7–12.
- 10. Birleşmiş Milletler Gıda ve Tarım Örgütü (FAO) (2012):
Croyoconservation of animal genetic resources. FAO Animal
Production and Health Guidelines No. 12. Rome.
- 11. Choi YH, Varner D, Love CC, Hartman DL, Hinrichs K
(2011): Production of live foals via intracytoplasmic injection
of lyophilized sperm and sperm extract in the horse.
Reproduction, 142: 529-538.
- 12. Derijik AA, Heijden GW, Giele M, Philippens ME, Van Bayel CC,
Boer P (2006): Gamma H2AX signaling during sperm chromatin
remodelling in the mouse zygote. DNA Repair 5: 959–971.
- 13. Ergün Z (2015). Biyolojik maddelerin kurutularak saklanması:
liyofilizasyon. Etlik Vet Mikrobiyoloji Dergisi, 26(1): 35-40.
- 14. Flosdorf EW, Mudd S (1935): Procedure and apparatus for
preservation in “lyophile” form of serum and other biological
substances. The J. of Immunology, 29(5): 389-425.
- 15. Flosdorf EW, Kımball AC (1939): Studies with H. Pertussis:
Maintenance of Cultures in Phase I. J Bacteriol 35: 696–704.
- 16. Gianaroli L, Magli MC, Stanghelline I, Crippa A, Crivello B,
Pescatorı ES, Ferrarettı AP (2012): DNA integrity is maintained
after freeze-drying of human spermatozoa. Fertility and
sterility, 97(5): 1067-1073.
- 17. Gil L, Olaciregui M, Luño V, Malo C, González N, Martínez
F (2014). Current Status of Freeze-Drying Technology to
Preserve Domestic Animals Sperm. Reproduction in Domestic
Animals, 49: 72–81.
- 18. Hara H, Tagiri M, Hwang IS, Takahashi M, Hirabayashi M, Hochi
S (2014): Adverse effect of cake collapse on the functional integrity
of freeze-dried bull spermatozoa. Cryobiology, 68(3):
354–360.
- 19. Harrouk W, Codrington A, Vinson R, Robaire B, Hales BF (2000):
Paternal exposure to cyclophosphamide induces DNA damage
and alters the expression of DNA repair genes in the rat
preimplantation embryo. Mutation Research, 461: 229–241.
- 20. Hochi S, Abdalla H, Hara H, Hirabayashi M (2011): Challenging
endeavour for preservation of freeze-dried mammalian spermatozoa.
Journal of Reproduction and Development, 57(5):
557-563.
- 21. Jaroudi S, Kakourou G, Cawood S, Doshi A, Ranieri DM,
Serhal P (2009): Expression profiling of DNA repair genes in
human oocytes and blastocysts using microarrays. Human
Reproduction, 24: 2649–2655.
- 22. Kaneko T, Whittingham DG, Yanagimachi R (2003a): Effect
of pH value of freeze-drying solution on the chromosome
integrity and developmental ability of mouse spermatozoa.
Biology of reproduction, 68(1): 136-139.
- 23. Kaneko T, Whittingham DG, Overstreet JW, Yanagimachi R
(2003b): Tolerance of the mouse sperm nuclei to freeze-drying
depends on their disuliyofilizeide status. Biology of
reproduction, 69(6): 1859-1862.
- 24. Kaneko T, Nakagata N (2006): Improvement in the long-term
stability of freeze-dried mouse spermatozoa by adding of a
chelating agent. Cryobiology, 53(2): 279-282.
- 25. Kaneko T, Serikawa T (2012): Successful long-term peservation
of rat sperm by freeze-drying. Cryobiology, 64(3): 211-
214.
- 26. Kaneko T, Ito H, Sakamoto H, Onuma M, Inoue-Murayama M
(2014): Sperm preservation by freeze-drying for the conservation
of wild animals. PloS one, 9(11): e113381.
- 27. Kaneko T (2016): Sperm freeze-drying and micro-insemination
for biobanking and maintenance of genetic diversity in
mammals. Reproduction, Fertility and Development, 28(8):
1079-1087.
- 28. Kawase Y, Araya H, Kamada N, Jishage K, Suzuki H (2005):
Possibility of long-term preservation of freeze-dried mouse
spermatozoa. Biology of reproduction, 72(3): 568-573.
- 29. Keskintepe L, Pacholczyk G, Machnicka A, Norris K, Curuk
MA, Khan I, Brackett BG (2002): Bovine blastocyst development
from oocytes injected with freeze-dried spermatozoa.
Biology of Reproduction, 67(2): 409–415.
- 30. Keskintepe L, Eroğlu A (2015): Freeze-drying of Mammalian
Sperm. Cryopreservation and Freeze-Drying Protocols. Ed.:
Wolkers WF, Oldenof H, p:489-497.
- 31. Krokida MK, Karathanos VT, Maroulis ZB (1998): Effect of
freeze-drying conditions on shrinkage and porosity of dehydrated
agricultural products. Journal of Food Engineering,
35(4): 369–380.
- 32. Kusakabe H, Yanagimachi R, Kamiguchi Y (2007): Mouse and
human spermatozoa can be freeze-dried without damaging
their chromosomes. Human Reproduction, 23(2): 233–239.
- 33. Kwon IK, Park KE, Niwa, K (2004): Activation, pronuclear formation,
and development in vitro of pig oocytes following
intracytoplasmic injection of freeze-dried spermatozoa.
Biology of Reproduction, 71(5): 1430–1436.
- 34. Liu JL, Kusakabe H, Chang CC, Suzuki H, Schmidt DW, Julian
M, Yang X (2004): Freeze-dried sperm fertilization leads to
full-term development in rabbits. Biology of Reproduction,
70(6): 1776–1781.
- 35. Lopez-fernandez C, Fernandez JL, Gosalbez A, Arroyo F, Vazquez
JM, Holt WV, Gosalvez J (2008): Dynamics of sperm DNA fragmentation
in domestic animals: Ram. Theriogenology, 70(6):
898-908.
- 36. Malik A, Laily M, Zakir MI (2015): Effects of long term storage
of semen in liquid nitrogen on the viability, motility and
abnormality of frozen thawed Frisian Holstein bull spermatozoa.
Asian Pacific Journal of Reproduction, 4(1): 22–25.
- 37. Men NT, Kikuchi K, Furusawa T, Dang-Nguyen TQ, Nakai M,
Fukuda A, Tajima A (2016): Expression of DNA repair genes
in porcine oocytes before and after fertilization by ICSI using
freeze-dried sperm. Animal Science Journal, 87(11): 1325–
1333.
- 38. Morishita N, Ochi M, Horiuchi T (2019): Development of
golden hamster embryos effectively produced by injection
of sperm heads sonicated in Tris-HCl buffer with EGTA.
Reproductive Medicine and Biology, 18(1): 83–90.
- 39. Nireeaha GR, Divya L, Sowmya C, Venkateshan N, Babu MN,
Lavakumar V (2013): Lyophilization/freeze drying—an review.
International Journal of Novel Trends in Pharmaceutical
Sciences, 3(4): 87-98.
- 40. Nur Z, Birler S, Üstüner B, Zık B, Özdaş ÖB, Akkoç CÖ
(2009-2012). Koç spermasının liyofilizasyonu ve intra-stoplazmik
sperm enjeksiyonu yöntemi ile embriyo üretimi.
(Yayınlanmamış veriler) TÜBİTAK Projesi, TEBAG 108R018.
- 41. Olaciregui M, Luño V, Gonzalez N, De Blas I, Gil L (2015):
Freeze-dried dog sperm: Dynamics of DNA integrity.
Cryobiology, 71(2): 286–290.
- 42. Olaciregui M, Luño V, Martí JI, Aramayona J, Gil L (2016):
Freeze-dried stallion spermatozoa: evaluation of two chelating
agents and comparative analysis of three sperm DNA
damage assays. Andrologia, 48(9): 988–994.
- 43. Olaciregui M, Luño V, Domingo P, González N, Gil L (2017):
In vitro developmental ability of ovine oocytes following
intracytoplasmic injection with freeze-dried spermatozoa.
Scientific Reports, 7(1): 1096.
- 44. Polge C, Smith AU, Parkes AS (1949): Revival of spermatozoa
after vitrification and dehydration at low temperatures.
Nature, 164(4172): 666.
- 45. Ringleb J, Waurich R, Wibbelt G, Streich WJ, Jewgenow K
(2011): Prolonged storage of epididymal spermatozoa does
not affect their capacity to fertilise in vitro-matured domestic
cat (Felis catus) oocytes when using ICSI. Reproduction,
Fertility and Development, 23(6): 818.
- 46. Shackell LF (1909): An improved method of desiccation,
with some applications to biological problems. Arn J
Physiol 20: 325–340.
- 47. Shahba MI, El-Sheshtawy RI, El-Azab AS, Abdel-Ghaffar AE,
Zıada MS, Zaky AA (2016): The effect of freeze-drying media
and storage temperature on ultrastructure and DNA of freeze-
dried buffalo bull spermatozoa. Asian Pacific Journal of
Reproduction, 5(6): 524-535.
- 48. Sherman JK (1954): Freezing and freeze-drying of human
spermatozoa. Fertility and sterility, 5(4): 357-371.
- 49. Şen U (2013): Liyofilize spermanın in vitro embriyo üretiminde
kullanımı. 8.Ulusal Zootekni Bilim Kongresi, 5-7 Eylül, Bildiri
Kitabı, s:183-188.
- 50. Wakayama T, Yanagimachi R (1998): Development of normal
mice from oocytes injected with freeze-dried spermatozoa.
Nature Biotechnology, 16(7): 639–641.
- 51. Wossidlo M, Arand J, Sebastiano V, Lepikhov K, Boiani M,
Reinhardt R, Walter J (2010): Dynamic link of DNA demethylation,
DNA strand breaks and repair in mouse zygotes. The
EMBO journal, 29(11): 1877-1888.
- 52. Yanagımachi R (1994): Mammalian fertilization. The physiology
of reproduction, Knobil E, Neill JD, Eds.: Raven Press,
p:189-317.
- 53. Yavaş İ, Cantekin Z, Korkmaz Yavaş T (2013): Sıvı azot ile yapılan
kryopreservasyon tekniklerinde mikrobiyal kontaminasyon
riskleri ve çözüm önerileri. Lalahan Hay. Arşt. Enst. Derg.
53(1): 39-45
- 54. Yöney T (2005): Gıdaları dondurarak kurutup saklamak nasıl
çalışır? Bilim teknik dergisi, 12:101.