In Vitro Fermentation Characteristics of Camelina Meal Comparison with Soybean Meal

The search for new and cheap sources of protein has been increased lately. Although camelina meal has antinutritive factors; compare to soybean it can be widely useable. The objective of this study is to remove the question mark in minds about camelina meal and to determine the fermentation characteristics parameters including pH, ammonia-N level, volatile fatty acids concentration as well as total gas volume, methane proportion and the estimated degradation of camelina meal in comparison with soybean meal. Basically, we used in vitro gas production system according to modified Hohenheim Gas Test (HFT) to compare camelina meal and soybean meal. Rumen contents obtained from two Holstein cows. There was no significant difference of pH and ammonia-N concentration between soybean meal and camelina meal, whereas total volatile fatty acid and acetate concentration were reduced in camelina meal. Additionally, total gas production, fermentative CO2 and estimated ME and organic matter digestibility were not altered. However, methane production decreased significantly in camelina meal fermenters. Consequently, it was concluded that camelina meal can be replaced of soybean meal, since microbial fermentation does not change and it might reduce the methane emission in which has commonly major effect on environmental pollution as a sera gas.

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  • Abdl-Rahman, M. A. (2010). In vitro Manipulation of Rumen Fermentation Efficiency by Fumaric acid – Bentonite Coupled Addition as an Alternative to Antibiotics. Journal of Agricultural Science, 2(2), p174. https://doi.org/10.5539/jas.v2n2p174
  • Alves, F. J. L., Ferreira, M. de A., Urbano, S. A., de Andrade, R. de P. X., da Silva, Á. E. M., de Siqueira, M. C. B., … Silva, J. D. L. (2016). Performance of lambs fed alternative protein sources to soybean meal. Revista Brasileira de Zootecnia, 45(4), 145–150. https://doi.org/10.1590/S1806-92902016000400001
  • Bayat, A. R., Kairenius, P., Stefański, T., Leskinen, H., Comtet-Marre, S., Forano, E., … Shingfield, K. J. (2015). Effect of camelina oil or live yeasts (Saccharomyces cerevisiae) on ruminal methane production, Rumen fermentation, And milk fatty acid composition in lactating cows fed grass silage diets. Journal of Dairy Science, 98(5), 3166–3181. https://doi.org/10.3168/jds.2014-7976
  • Brandao, V. L. N., Silva, L. G., Paula, E. M., Monteiro, H. F., Dai, X., Lelis, A. L. J., … Faciola, A. P. (2018). Effects of replacing canola meal with solvent-extracted camelina meal on microbial fermentation in a dual-flow continuous culture system. Journal of Dairy Science, 101(10), 9028–9040. https://doi.org/10.3168/jds.2018-14826
  • Chaney, A. L., Marbach, E. P. (1962). Modified reagents for determination of urea and ammonia. Clinical Chemistry, 8(2), 130-132.
  • Colombini, S., Broderick, G. A., Galasso, I., Martinelli, T., Rapetti, L., Russo, R., & Reggiani, R. (2014). Evaluation of Camelina sativa (L.) Crantz meal as an alternative protein source in ruminant rations. Journal of the Science of Food and Agriculture, 94(4), 736–743. https://doi.org/10.1002/jsfa.6408
  • Ebeid, H. M., Hassan, F., Li, M., Peng, L., Peng, K., Liang, X., & Yang, C. (2020). Camelina sativa L. Oil Mitigates Enteric in vitro Methane Production, Modulates Ruminal Fermentation, and Ruminal Bacterial Diversity in Buffaloes. Frontiers in Veterinary Science, 7, 550. https://doi.org/10.3389/fvets.2020.00550
  • Florou-Paneri, P., Christaki-Sarikaki, E., Peng, J., Tsinas, A., Skoufos, I., Bonos, E., … Tzora, A. (2014). Alternative protein sources to soybean meal in pig diets. Journal of Food, Agriculture & Environment, 12, 655–660. https://doi.org/10.1234/4.2014.5214
  • Geissler, C., Hoffmann, M., Hickel, B. (1976). Gas chromatographic estimation of volatile fatty acids. Archives of Animal Nutrition, 26, 123-129.
  • Haddad, S. G. (2006). Bitter vetch grains as a substitute for soybean meal for growing lambs. Livestock Science, 99(2–3), 221–225. https://doi.org/10.1016/j.livprodsci.2005.06.014
  • Halmemies-Beauchet-Filleau, A., Shingfield, K. J., Simpura, I., Kokkonen, T., Jaakkola, S., Toivonen, V., & Vanhatalo, A. (2017). Effect of incremental amounts of camelina oil on milk fatty acid composition in lactating cows fed diets based on a mixture of grass and red clover silage and concentrates containing camelina expeller. Journal of Dairy Science, 100(1), 305–324. https://doi.org/10.3168/jds.2016-11438
  • Hao, X. Y., Yu, S. C., Mu, C. T., Wu, X. D., Zhang, C. X., Zhao, J. X., & Zhang, J. X. (2020). Replacing soybean meal with flax seed meal: Effects on nutrient digestibility, rumen microbial protein synthesis and growth performance in sheep. Animal, 14(9), 1841–1848. https://doi.org/10.1017/S1751731120000397
  • Lawrence, R. D., Anderson, J. L., & Clapper, J. A. (2016). Evaluation of camelina meal as a feedstuff for growing dairy heifers. Journal of Dairy Science, 99(8), 6215–6228. https://doi.org/10.3168/jds.2016-10876
  • Menke, H.H. & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55. Retrieved from https://ci.nii.ac.jp/naid/10025840911
  • Moriel, P., Nayigihugu, V., Cappellozza, B. I., Gonçalves, E. P., Krall, J. M., Foulke, T., … Hess, B. W. (2011). Camelina meal and crude glycerin as feed supplements for developing replacement beef heifers. Journal of Animal Science, 89(12), 4314–4324. https://doi.org/10.2527/jas.2010-3630
  • Paula, E. M., da Silva, L. G., Brandao, V. L. N., Dai, X., & Faciola, A. P. (2019, October 1). Feeding canola, camelina, and carinata meals to ruminants. Animals, Vol. 9, p. 704.
  • Russo, R., & Reggiani, R. (2017). Glucosinolates and Sinapine in Camelina Meal. Food and Nutrition Sciences, 08(12), 1063–1073. https://doi.org/10.4236/fns.2017.812078
  • Salas, H., Castillejos, L., López-Suárez, M., & Ferret, A. (2019). In Vitro Digestibility, In Situ Degradability, Rumen Fermentation and N Metabolism of Camelina Co-Products for Beef Cattle Studied with a Dual Flow Continuous Culture System. Animals, 9(12), 1079. https://doi.org/10.3390/ani9121079
  • Sizmaz, O., Calik, A., Sizmaz, S., & Yildiz, G. (2016). A comparison of camelina meal and soybean meal degradation during incubation with rumen fluid as tested in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63, 157-161.
  • Vincent, I. C., Hill, R., & Williams, H. L. (Dep. V. M. and A. H. T. R. V. C. B. P. P. B. H. E. 1NB (UK)). (1988). Rapeseed meal in the diet of pubertal heifers during early pregnancy. Animal Production (UK). Retrieved from https://agris.fao.org/agris-search/search.do?recordID=GB8901689
  • Wang, S., Kreuzer, M., Braun, U., & Schwarm, A. (2017). Effect of unconventional oilseeds (safflower, poppy, hemp, camelina) on in vitro ruminal methane production and fermentation. Journal of the Science of Food and Agriculture, 97(11), 3864–3870. https://doi.org/10.1002/jsfa.8260
  • Waraich, E. A., Ahmed, Z., Ahmad, R., Yasin Ashraf, M., Saifullah, Naeem, M. S., & Rengel, Z. (2013). Camelina sativa, a climate proof crop, has high nutritive value and multiple-uses: A review. Australian Journal of Crop Science, 7(10), 1551–1559.
  • Wolin, M. J. (1960). A theoretical rumen fermentation balance. Journal of Dairy Science, 43(10), 1452–1459. https://doi.org/10.3168/jds.S0022-0302(60)90348-9
  • Zagorakis, K., Liamadis, D., Milis, C., Dotas, V., Dotas, D. (2015). Nutrient digestibility and in situ degradability of alternatives to soybean meal protein sources for sheep. Small Ruminant Research, 124, 38-44.