Lemon Peel Extract for Synthesizing Non-Toxic Silver Nanoparticles through One-Step Microwave-Accelerated Scheme

In this study, biofunctionalized globular or oval shaped silvernanoparticles were obtained by using aqueous extract of lemon peel(Citrus limon) via microwave-accelerated heating system. WhileUV−visible spectroscopy, FTIR and XRD analyses were applied torecognize the formation of nano-silver, TEM and Zeta analysis wereemployed to reveal their morphological features. UV–vis spectrum offabricated AgNPs indicated its characteristic maximum absorbance at445 nm. Phytosynthesized silver nanoparticles were poly-dispersedwith Z-average value of 41.86 nm, and showed excellent stability forseveral months with no aggregation and agglomeration. The non-toxicnature of the developed Ag nanoparticles was further confirmed byapplying on healthy mouse fibroblast L929 cell line, which mayexpand their potentials for further studies related to medical scienceand other biological applications.

Mikrodalga Hızlandırmalı Yöntemi İle Limon Kabuk Ekstraktsiyonu Kullanarak Non-toksik Gümüş Nanopartiküllerin Sentezlenmesi

Bu çalışmada, mikrodalga hızlandırmalı ısıtma sistemi yöntemi ile limon kabuk (Citrus limon) ekstraktsiyonu kullanarak biyofonksiyonel küre şeklinde veya oval şekilli gümüş nanopartiküller elde edilmiştir. Çalışmada gümüş nanopartiküllerin oluşumunu tanımak için UV–vis absorpsiyon spektroskopi, FTIR ve XRD analizleri uygulanırken, nanopartiküllerin morfolojik özelliklerini ortaya çıkarmak için TEM ve Zeta analizi uygulanılmıştır. Üretilmiş AgNP’lerin UV-vis spektrumu, 445 nm’de karakteristik maksimum absorbansını göstermiştir. Fitosentezlenmiş gümüş nanopartiküller, Z-ortalama değeri 41,86 nm olan poli-dispersiyon haline getirilmesi ile birlikte herhangi bir agregasyon ve agglomerasyon olmadan birkaç ay boyunca mükemmel stabilite sergilemiştir. Daha sonra, sağlıklı fare fibroblast hücreleri (L929 hücre çizgisi) üzerindeki biyosentezlenmiş bu Ag NP’lerin non-toksik özelliği doğrulanmıştır. Bu durum, tıp bilimlerin yanı sıra çeşitli biyolojik uygulamalarda da bu nanopartiküllerin potansiyellerini göstermektedir.

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