Elif AYAZOĞLU DEMİR, SELİM DEMİR, İbrahim TURAN

Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

Ethyl pyruvate (EP) is a simple aliphatic ester derived from pyruvicacid which is an endogenous metabolite. Although various studieshave investigated the antioxidant and anti-inflammatory propertiesof EP, there has been only limited research into the cytotoxic effect ofEP on cancer cells. The aim of this study was to determine thecytotoxic effects of EP on cells representing common cancer types. EPwas purchased commercially and intermediate stock solutions wereprepared with phosphate buffer saline. The cytotoxic effect of EP onhuman melanoma (VMM917), cervix (HeLa), breast (MCF-7), lung(A549), liver (HepG2), colon (WiDr) cancer and normal fibroblast (BJ)cells was determined using the MTT assay. Cisplatin was used as apositive control in cytotoxicity experiments. The results showed thatEP exhibits selective cytotoxic effect on VMM917 (10.1-fold) and HeLa(3.04-fold) cells compared to BJ cells. This study shows for the firsttime that EP has a highly selective cytotoxic effect, especially onmelanoma and cervix cancer cells. The mechanism of this effect needsto be elucidated by more extensive studies.

Etil Piruvatın Çeşitli Kanser Hücre Hatları Üzerindeki Sitotoksik Etkisinin İncelenmesi

Etil piruvat (EP), endojen bir metabolit olan pirüvik asitten türetilen basit bir alifatik esterdir. Çeşitli çalışmalar EP'nin antioksidan ve anti-inflamatuar özelliklerini ortaya koymuş olmasına rağmen, EP'nin kanser hücreleri üzerindeki sitotoksik etkisi hakkında sınırlı sayıda araştırma bulunmaktadır. Bu çalışmanın amacı, EP'nin yaygın kanser türlerini temsil eden hücreler üzerindeki sitotoksik etkilerini belirlemektir. Çalışmada kullanılan EP ticari olarak satın alınmış ve ara stok çözeltiler fosfat tamponu ile hazırlanmıştır. EP'nin insan melanoma (VMM917), serviks (HeLa), meme (MCF-7), akciğer (A549), karaciğer (HepG2), kolon (WiDr) kanseri ve normal fibroblast (BJ) hücreleri üzerindeki sitotoksik etkisi MTT testi kullanılarak belirlenmiştir. Sitotoksisite deneylerinde pozitif kontrol olarak sisplatin kullanılmıştır. Sonuçlar EP'nin BJ hücrelerine kıyasla VMM917 (10.1 kat) ve HeLa (3.04 kat) hücreleri üzerinde seçici sitotoksik etki gösterdiğini ortaya koymuştur. Bu çalışma, EP’nin özellikle melanoma ve serviks kanseri hücreleri üzerinde oldukça seçici bir sitotoksik etkiye sahip olduğunu ilk kez göstermektedir. Bu etkinin mekanizmasının daha kapsamlı çalışmalarla açıklanması gerekmektedir.

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