Sara YASEMİN, Ayşi̇n GÜZEL DEĞER, Sertan ÇEVİK, Nezihe KÖKSAL

Benchmarking of the Effects of Salinity on Antioxidant Enzymes Activities, Lipid Peroxidation and H2O2 Levels in the Leaves of Two Zinnia Species

In this study, it was aimed to investigate the effects of salinity, whichis an important environmental problem, in the cultivation ofornamental plants (such as zinnia) and irrigation with high saltwater, especially on the antioxidant defense mechanism. For thispurpose, the two Zinnia species were irrigated by differentconcentrations of saline water (50, 100, 150, 200 mM NaCl); effects ofsalinity on superoxide dismutase (SOD), catalase (CAT), glutathionereductase (GR) lipid peroxidation (MDA) and hydrogen peroxide(H2O2) in the leaves were determined. The results showed that salinityconspicuously increased SOD, CAT, GR, H2O2 and MDA content attwo Zinnia species compared to the control groups. It was found thatSOD and CAT enzyme activities increased remarkably with 150 mMNaCl in both Zinnia species, but decreased with 200 mMNaCl.The highest GR enzyme activity was observed in 200mM saltconcentration at Zinnia marylandica ‘Double Zahara Fire Improved’.MDA and H2O2 levels were observed higher in Zinnia elegans ‘ZinnitaScarlet’.To conclude; it may be said that these two Zinnia varieties cantolerate salt concentration up to 150 mM.

İki Zinnia Türünün Yapraklarında Tuzluluğun Antioksidan Enzim Aktiviteleri, Lipid Peroksidasyonu ve H2O2 Düzeyleri Üzerine Etkilerinin Karşılaştırılması

Bu çalışmada, önemli bir çevresel sorun olan tuzluluğun, süs bitkilerinin (zinnia gibi) yetiştirilmesinde ve yüksek tuzlu su ile sulamada, özellikle antioksidan savunma mekanizması üzerindeki etkilerinin araştırılması amaçlanmıştır. Bu amaçla, iki Zinnia türü farklı konsantrasyonlarda tuzlu su (50, 100, 150, 200 mM NaCl) ile sulanmıştır; tuzluluğun yapraklardaki süperoksit dismutaz (SOD), katalaz (CAT), glutatyon redüktaz (GR) lipit peroksidasyonu (MDA) ve hidrojen peroksit (H2O2) üzerindeki etkileri belirlenmiştir. Sonuçlar, tuzluluğun iki Zinnia türünde SOD, CAT, GR, H2O2 ve MDA içeriğini kontrol gruplarına kıyasla belirgin şekilde arttırdığını göstermiştir. SOD ve CAT enzim aktivitelerinin her iki Zinnia türünde 150 mM NaCl ile önemli ölçüde arttığı, ancak 200 mM NaCl ile azaldığı bulunmuştur. En yüksek GR enzim aktivitesi, Zinnia marylandica "Double Zahara Fire Improved" da 200mM tuz konsantrasyonunda gözlenmiştir. MDA ve H2O2 seviyeleri Zinnia elegans 'Zinnita Scarlet' da daha yüksek olarak gözlemlenmiştir. Sonuç olarak, bu iki Zinnia çeşidinin 150 mM'a kadar tuz konsantrasyonunu tolere edebildiği söylenebilir.

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