Samsun İli ve Çevresindeki Atopik Dermatitisli Köpeklerde Serum Spesifik IgE Tespiti ile Alerjen Tayini

Bu çalışma ile Samsun ili ve çevresindeki Atopik Dermatitis (AD)’li köpeklerde ilk kez serum spesifik immunoglobin E (IgE) ile alerjen profillerinin belirlenmesi amaçlanmıştır. Değişik ırk, cinsiyet ve yaşlardaki 21 adet köpeğe Favrot Kriterleri ile AD teşhisi konulmuştur. Köpeklere ait serum örnekleri serolojik olarak 20 farklı alerjene spesifik IgE yönünden incelenmiştir. AD’li köpeklerde 20 farklı alerjenin görülme oranlarına cinsiyet, yaş ve beslenme şekillerinin etkisi araştırılmıştır. Samsun ili ve çevresindeki AD’li köpeklerdeki farklı alerjen profilleri arasında en yüksek %95 oranında ev tozu akarı Dermatophagoides farinae seropozitifliği saptanmıştır. Bununla birlikte köpeklerde farklı oranlarda diğer alerjenlere yönelik seropozitiflik saptanmıştır. Dişi köpeklerde erkek köpeklere oranla sadece Ctenocephalides spp. seropozitifliğinin yüksek olduğu istatistiksel olarak tespit edilmiştir (p<0,05). Bununla birlikte AD’li köpeklerde yaş ve beslenme şekillerinin alerjen görülme sıklığına etkisinin olmadığı tespit edilmiştir (p>0,05). Samsun ili ve çevresindeki AD’li köpeklerde ev tozu ile depo akarlarının serum spesifik IgE değerlerinin çok yüksek oranlarda pozitiflik verdiği saptanmıştır.

Anahtar Kelimeler:

Alerjen, Atopik dermatitis, Köpek, Samsun, Spesifik IgE

Allergen Determination with Detection of Serum Specific IgE in Dogs with Atopic Dermatitis from Samsun and Vicinity

It is aimed to detect allergen determination via serum specific immunoglobulin E (IgE) for the first time in dogs diagnosed as Atopic Dermatitis (AD) from Samsun and vicinity within this present study. Twenty one dogs with different age, breed and gender diagnosed with AD based up on the Favrot’s criteria. Serum samples of the dogs were analyzed by detection of serum specific IgE for determination of 20 different allergens. The effects of gender, age and the feeding routes for those 20 different allergens separately were analyzed. Based up on the grading of the allergens detected in 21 dogs with AD from Samsun and vicinity; results reflects that Dermatophagoides farinae was stated as first degree within a very high seropositivity rate (95%). Moreover, in varying degree of seropositivitiy rates of other allergens were also detected. Females showed statistically (p<0.05) higher positivity rate to the 20th allergen, Ctenocephalides spp. more than males. Furthermore, we did not found any significant effects of age and feeding routes on the allergens in the dogs with AD (p>0.05). Dogs with AD from Samsun and vicinity had very high serum specific IgE levels against to the house dust and storage mites.

Keywords:

Allergen, Atopic dermatitis, Dog, Samsun, Specific IgE,

PDF

___

Açıcı M, İğde M, Beyhan YE, Pekmezci GZ, Gürler AT, Hökelek M, Umur Ş. Ev ve işyerlerinde ev tozu ve akar faunası. Ondokuz Mayıs Üniv Tıp Fak Derg. 2009; 25(1): 7.
Carlotti DN, Costargent F. Analysis of positive skin tests in 449 dogs with allergic dermatitis. Prakt Med Chir An de Cie. 1992; 27: 53-69.
Chanthick C, Anaman S, Buathet K. The prevalence of positive intradermal allergy tests in 114 dogs with atopic dermatitis in the Bangkok metropolis, Thailand. Vet Immunol Immunopathol. 2008; 126: 256-262.
Codner EC, Tinker MK. Reactivity to intradermal injections of extracts of house dust and house dustmite in healthy dogs and dogs suspected of being atopic. J Am Vet Med Assoc. 1995; 206: 812-816.
De Weck AL. What can we learn from the allergic zoo? Int Arch Allergy Immunol. 1995; 107: 13-18.
Favrot C, Steffan J, Seewald W, Picco F. A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol. 2010; 21(1): 23-31.
Griffin CE, DeBoer DJ. The ACVD task force on canine atopic dermatitis (XIV): clinical manifestations of canine atopic dermatitis. Vet Immunol Immunopathol. 2001; 81(3-4): 255-269.
Halliwell RE, Schwartzman RM, Rockey JH. Antigenic relationship between human IgE and canine IgE. Clin Exp Immunol. 1972; 10(3): 399-407.
Hammerling R, De Weck AL. Comparison of two diagnostic tests for canine atopy using monoclonal anti-IgE antibodies. Vet Dermatol. 1998; 9: 191-199.
Hill PB, DeBoer DJ. The ACVD task force on canine atopic dermatitis (IV): environmental allergens. Vet Immunol Immunopathol. 2001; 81: 169-186.
Jeffers JG, Shanley KJ, Meyer EK. Diagnostic testing of dogs for food hypersensitivity. J Am Vet Med Assoc. 1991; 198(2): 245-250.
Kang MH, Kim HJ, Jang HJ, Park HM. Sensitization rates of causative allergens for dogs with atopic dermatitis: detection of canine allergen-specific IgE. J Vet Sci. 2014; 15: 545-550.
Kim HJ, Kang MH, Park HM. Common allergens of atopic dermatitis in dogs: comparative finings based on intradermal tests. J Vet Sci. 2011; 12: 287-290.
Masuda K, Sakaguchi M, Fujiwara S, Kurata K, Yamashita K, Odagiri T, Nakao Y, Matsuki N, Ono K, Watari T, Hasegawa A, Tsujimoto H. Positive reactions to common allergens in 42 atopic dogs in Japan. Vet Immunol Immunopathol. 2000; 73: 193-204.
Mueller RS, Bettenay SV, Tideman L. Aero-allergens in canine atopic dermatitis in southeastern Australia based on 1000 intradermal skin tests. Aust Vet J. 2000; 78: 392-399.
Nesbitt GH. Canine allergic inhalant dermatitis: a review of 230 cases. J Am Vet Med Assoc. 1978; 172: 55-60.
Nesbitt GH, Kedan GS, Cacciolo P. Canine atopy. Ethiology and diagnosis. Comp Cont Educ Pract. 1984; 6: 73-85.
Nødtvetd A, Bergvall K, Sallander M, Egenvall A, Emanuelson U, Hedhammar A. A case‐control study of risk factors for canine atopic dermatitis among boxer, bullterrier and West Highland white terrier dogs in Sweden. Vet Dermatol. 2007; 18: 309-315.
Prelaud P, Guague ÁE, Alhaidari Z, Faivre N, Heripret D. Reevaluation of diagnostic criteria of canine atopic dermatitis. Rev Med Vet. 1998; 149: 1057-1064.
Rosser Jr EJ. Diagnosis of food allergy in dogs. J Am Vet Med Assoc. 1993; 203(2): 259-262.
Roussel AJ, Bruet V, Bourdeau PJ. Characterisation of dog sensitisation to grass pollen in western France from 1999 to 2010. Vet Rec. 2013; 172: 686.
Saridomichelakis MN, Koutinas AF, Gioulekas D, Leontidis L. Canine atopic dermatitis in Greece: clinical observations and the prevalence of positive intradermal test reactions in 91 spontaneous cases. Vet Immunol Immunopathol. 1999; 69: 61-73.
Scott DW. Observations on canine atopy. J Am Anim Hosp Assoc. 1981; 17: 91-100.
Scott DW, Miller WH, Griffin CE. Muller and Kirk’s Small Animal Dermatology 5th edn. Philadelphia: W.B. Saunders, 1995; pp. 500-518.
Sousa CA, Norton AL. Advances in methodology for diagnosis of allergic skin disease. Vet Clin Small Anim Pract. 1990; 20(6): 1419-1427.
Sture GH, Halliwell RE, Thoday KL, van den Broek AH, Henfrey JI, Lloyd DH, Mason IS, Ferguson E. Canine atopic disease: the prevalence of positive intradermal skin tests at two sites in the north and south of Great Britain. Vet Immunol Immunopathol. 1995; 44: 293-308.
Temizel EM, Aytug N. Intradermal testing results and clinical features in dogs with atopic dermatitis in Turkey. J Biol Environ Sci. 2011; 5: 87-89.
Willemse T, van den Brom WE. Investigation of the symptomatology and the significance of immediate skin test reactivity in canine atopic dermatitis. Res Vet Sci. 1983; 34: 261-265.
Willemse T, van den Brom WE, Rijnberk A. Effect of hyposensitization on atopic dermatitis in dogs. J Am Vet Med Assoc. 1984; 184: 1277-1280.
Zunic M. Comparison between immunodot tests and the intradermal skin test in atopic dogs. Vet Dermatol. 1998; 9: 201-205.
Zur G, Ihrke PJ, White SD, Kass PH. Canine atopic dermatitis: a retrospective study of 266 cases examined at the University of California, Davis, 1992-1998. Part I. Clinical features and allergy testing results. Vet Dermatol. 2002; 13(2):89-102.