TEOMAN ZAFER APAN, Murat YIDIRIM, MEHMET BAŞALAN, AYLİN KASIMOĞLU

Kırıkkale Yöresinde İnsan ve Hayvan Kaynaklarından İzole Edilen E. coli O157:H7’nin Prevalansı

Amaç: Escherichia coli O157:H7 suşu hemorajik kolite neden olmakta ve çok düşük konsantrasyonlarda bile ciddi salgınlara yol açabilmektedir. Bulaşma kontamine yiyeceklerin fekal-oral yol ile alınması ile gerçekleşmekte fakat doğrudan insan teması ile de görülebilmektedir. Ülkemizde E. coli O157:H7 prevalansı genel olarak bilinmemekle beraber Kırıkkale bölgesinde de bilinmemektedir. E. coli O157:H7 prevalansını belirlemek amacı ile Kırıkkale bölgesinde insan, besi hayvanı, yem ve et örnekleri bir yıl süre ile araştırıldı.Gereç ve Yöntemler: Bütün örnekler mümkün olan en kısa sürede doğru ve steril şartlarda laboratuvara getirildi. E. coli O157:H7 izolasyonunda 89 gastroenteritli hastanın dışkı örneği, 108 sığır dışkısı, 69 değişik hayvan yemi ve 84 et (kıyma) örneği klasik kültürü takiben Dynabead ve ELISA metotları kullanılarak belirlendi. Kıyma örnekleri çalışma testleri yapılıncaya kadar -70 derecede bekletildi.Bulgular: E. coli O157:H7 araştırılan insan ve hayvanlarda, yem veya et (kıyma) örneklerinde tespit edilemedi. Sonuç: Bulgularımız ahırdan insana uzanan besin zincirinin E. coli O157:H7 suşu açısından güvenli olduğunu göstermiştir. Öte yandan diğer besin ve su kaynakları bu mikroorganizma için potansiyel kaynaklar olabilir.

Anahtar Kelimeler:

Escherichia coli, ELISA, seroprevalans, insan, sığır, ELISA

PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE

Objective: Escherichia coli O157:H7 strain is a cause of hemorrhagic colitis and may give rise to severe outbreaks even at a low concentration. Transmission may occur through fecal-oral route with contaminated food but direct transmission from personal contact is also possible. Presence of E. coli O157:H7 was investigated in humans, cattle, animal feed, and ground beef over a one-year year period in order to determine the prevalence in the Kırıkkale region.Material and Methods: All samples were transferred to the microbiology laboratory as rapidly as possible under appropriate and sterile conditions. The isolation of E. coli O157:H7 was performed by serotyping with Dynabeads and ELISA methods in stool specimens in 89 patients with gastroenteritis, 108 cattle, 69 different animal broth samples, and 84 samples from ground beef after culture using classical methods. Minced meat samples were kept at -70 degrees until working tests.Results: E. coli O157:H7 was not detected in humans or animals or sources including animal feed and beef carcasses. Conclusion: Our results indicate that the meat chain from cattle to humans is safe with respect to the E. coli O157:H7 strain. On the other hand, other food or water sources may be potential sources for this microorganism.

Keywords:

Escherichia coli, seroprevalence, human, cattle, ELISA, ELISA,

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