DÜŞÜK VE YÜKSEK OVER REZERVLİ VAKALARDA VEGF POLİMORFİZM SIKLIĞININ KIYASLANMASI

Amaç: Vasküler endotelyal büyüme faktörü (VEGF), overyan anjiogenezde kilit rol oynayan bir sitokindir. Biz de çalışmamızda düşük ve yüksek over rezervli infertil kadınlarda VEGF gen polimorfizminin over rezervi ile olası ilişkisini araştırmayı amaçladık.Gereç ve Yöntemler: Bu çalışmaya, polikistik over sendromlu (PCOS) 64 kadın ile prematür overyan yetmezlikli (POF) 30 kadın dahil edildi. Periferik venöz kan örneklerinden elde edilen DNA’da polimeraz zincir reaksiyonu (PCR) yöntemi ile VEGF geni-2578 C/A tek nükleotid polimorfizmi (SNP) incelendi. İstatistiki analiz SPSS 16.0 programı ile yapıldı.Bulgular: PCOS ve POF gruplarının ortalama yaş, vücut kitle indeksi, TSH ve prolaktin seviyeleri yönünden anlamlı bir fark yoktu. PCOS grubu için genotip dağılımı %56.2 CC, %26.6 CA ve %17.2 AA iken, POF grubu için ise %26.7 CC %53.3 CA ve %20 AA idi. Gruplar arasında genotip frekansları bakımından farklılık saptandı (p = 0.018). Mutant A allelinin, over rezervini minimal azaltıcı etkisi olduğu gözlendi (95%CI= 0.12-0.22, OR: 0.5, p<0.01).Sonuç: Çalışmamızda gözlediğimiz VEGF -2578 C/A polimorfizmindeki mutant A allelinin over rezervi üzerine olan etkisini tartışmak için genişletilmiş popülasyon çalışmalarına ihtiyaç vardır.

Anahtar Kelimeler:

VEGF polimorfizmi, over rezervi, prematür overyan yetmezlik

The Comparison of VEGF Polymorphism Frequency between Cases with Low and High Ovarian Reserve

Objective: Vascular endothelial growth factor (VEGF) is a cytokine playing key role in ovarian angiogenesis. We aimed to investigate the possible relation between VEGF gene polymorphism and ovarian reserve in infertile women suffering from either low or high ovarian reserve.Material and Methods: This study was conducted on 64 women suffering from polycystic ovary syndrome (PCOS) and 30 women suffering from premature ovarian failure (POF). VEGF-2578 C/A single nucleotide polymorphism (SNP) was investigated with polymerase chain reaction (PCR) method on DNA extracted from peripheral venous blood sample. Statistical analyses were done using SPSS 16.0 programme.Results: There was no significant difference between PCOS and POF groups for age, body mass index, TSH and prolactin levels. The genotype frequencies of PCOS and POF groups were respectively 56.2% CC, 26.6% CA, 17.2% AA and 26.7% CC, 53.3% CA, 20% AA. There was a significant difference between groups for genotype frequencies (p=0.018). We observed minimal negative influence of mutant A allele on ovarian reserve (95%CI= 0.12-0.22, OR: 0.5, p<0.01).Conclusion: We observed a minimal negative influence of VEGF-2578 C/A polymorphism on ovarian reserve. New and expanded population studies are needed to discuss our results.

Keywords:

VEGF polymorphism, premature ovarian failure, polycystic ovary syndrome,

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