Özge SÜFER, Fuat BOZOK, Hatıra TAŞKIN, Saadet BÜYÜKALACA

Türkiye’den Toplanan Cordyceps militaris’in Bazı Fiziksel ve Kimyasal Özellikleri

Çalışmanın amacı: Türk Cordyceps militaris’i, renk, doku, toplam fenolik düzeyi ve antioksidan aktivite gibi bazı kalite özelliklerinin belirlenmesi amacıyla toplanmıştır. Materyal ve metod: 2018 yılında İstanbul’daki bir araziden toplanan mantar örnekleri (kültür ortamında yetişmemiş, yabani) Eurx genematrix universal isolation kit kullanılarak toplam genomik DNA’nın belirlenmesi için analiz edilmiştir. C. militaris gövdesinin renk ve dokusal özellikleri sırasıyla bir renk ölçer (Munsell sistemine göre) ve doku analizörü (doku profili analizi, TPA uygulanarak) vasıtasıyla tayin edilmiştir. C. militaris’in metanol içerisinde hazırlanmış özütü, toplam fenolik tayinine (Folin Ciocalteu yöntemi) ve antioksidan aktivite testine (DPPH yöntemi) tabi tutulmuştur. Sonuçlar: L*, a*, b*, C*, H° (renk) değerleri sırasıyla 34.96, 18.84, 21.22, 28.41 ve 48.57 olarak kaydedilmiştir. Örneklerin sertliği 26.25 N’dir. Numunelerin toplam fenolik miktarı ve DPPH radikali süpürme aktivitesi 2316.22 mg gallik asit eşdeğeri (GAE)/L özüt ve 3.98 mg troloks eşdeğeri (TE)/L özüt olarak bulunmuştur. Önemli vurgular: Bu ön çalışmanın önerisi, C. militaris’in yüksek biyoaktif molekül ve antioksidan potansiyelinden dolayı, çeşitli gıda formülasyonlarında bir besin öğesi olmasının yararlı olabileceğidir.

Anahtar Kelimeler:

Biyolojik Aktivite, Cordycipitaceae, Tıbbi Makromantar

Some Physical and Chemical Properties of Fruitbodies of Cordyceps militaris Collected in Turkey

Aim of study: Turkish Cordyceps militaris was collected in order to determine its certain quality characteristics such as color, texture, total phenolics level and antioxidant activity. Material and method: Samples (not cultivated, wild) which were harvested from a field of Istanbul in 2018 were analyzed by using Eurx genematrix universal isolation kit for determining total genomic DNA. Color and textural properties of C. militaris body were measured by a colorimeter (according to Munsell system) and texture analyzer (applying texture profile analysis, TPA) respectively. The methanolic extract of C. militaris was subjected to total phenolic determination (by Folin Ciocalteu method) and antioxidant activity (by DPPH method) tests. Main results: L*, a*, b*, C*, H° (color) values were recorded as 34.96, 18.84, 21.22, 28.41 and 48.57 respectively. Hardness of samples was 26.25 N. Total phenolic amounts and DPPH radical scavenging activity of specimens were found as 2316.22 mg gallic acid equivalent (GAE)/L extract and 3.98 mg trolox equivalent (TE)/L extract. Highlights: The recommendation of this preliminary study is that C. militaris may be useful in various food formulations as an ingredient because of its high bioactive molecule and antioxidant potential.

Keywords:

Biological Activity, Cordycipitaceae, Medicinal Macrofungi,

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