YASEMİN ÇELİK ALTUNOĞLU, Kerim GÜNEY, Pinar BALOGLU, MEHMET CENGİZ BALOĞLU

Türk Göknar Taksonlarında Genetik Çeşitlilik Analizleri

Çalışmanın amacı: Beş Abies taksonu Türkiye'de doğal olarak dağılmıştır. Abies nordmanniana' nın üç alt türü ve A. cilica 'nın iki alt türü vardır. Bu çalışmada, cpDNA bölgeleri, trnR-trnN ve rps18-rpl20 bölgelerine göre hem Türk taksonlarında hem de dünyanın diğer Abies taksonlarında filogenetik ilişkileri göstermeyi amaçladık. Materyal ve yöntem: CTAB metodu ile DNA izolasyonunun ardından ilgili göknar DNA bölgeleri çoğaltılmış ve dizilenmiştir. Filogenetik ağaçlar, 1000 tekrarlı maximum likelihood (maksimum olabilirlik) yöntemi kullanılarak oluşturulmuştur. Temel sonuçlar: Türkiye’deki göknar taksonları arasında bu bölgeler bakımından ayrım yapmak zor gözükmektedir. rps18-rpl20 dizileri temelindeki filogenetik ağaca dayanarak, bazı Abies cilicica subsp. isaurica, A. nordmanniana subsp. equi-trojani ve A. cilicica subsp. cilicica üyeleri A. spectabilis ve A. densa ile aynı sınıfta yer alırken, bazı A. nordmanniana subsp. bornmulleriana, A. cilicica subsp. isaurica ve A. nordmanniana subsp. equi-trojani üyeleri ise Türkiye’ye yakın yayılış gösteren A. alba ve Kuzey Amerika’da yayılışı bilinen A. amabilis ile aynı sınıfta yer almıştır. Araştırma Vurguları: Mevcut çalışma, Türkiye’deki göknar türleri arasında cpDNA çeşitliliğinin dağılımına ve Türkiye göknarları ile dünyada yayılış gösteren diğer göknar türleri arasındaki genetik çeşitliliğe yeni bir ışık tutmaktadır.

Anahtar Kelimeler:

Göknar, Moleküler Taksonomi, Kloroplast DNA Bölgesi

Genetic Diversity Analysis of cpDNA in Turkish Abies Taxa

Aim of study: Five Abies taxa naturally distributed in Turkey. Abies nordmanniana has three subspecies and A. cilica has two subspecies. In this study, we aimed to show phylogenetic relationships both in Turkish taxa and in other Abies taxa from around the world based on cpDNA regions, trnR-trnN and rps18-rpl20 regions. Material and methods: Following CTAB-based DNA isolation method, the relevant fir DNA regions were amplified and sequenced. Phylogenetic trees were constructed using maximum likelihood method with 1000 bootstrap replicates. Main results: It was difficult to make distinctions among the Turkish Abies taxa based on the sequenced DNA regions. Based on rps18-rpl20 phylogenetic tree, some members of Abies cilicica subsp. isaurica, A. nordmanniana subsp. equi-trojani and A. cilicica subsp. cilicica were in the same clade with A. spectabilis and A. densa; However, some members of A. nordmanniana subsp. bornmuelleriana, A. cilicica subsp. isaurica and A. nordmanniana subsp. equi-trojani were placed in a clade with A. alba placed near Turkey and A. amabilis known from North America. Highlights: This study provides new insights into the distribution of cpDNA variation in Abies species in Turkey and the genetic variation between firs in Turkey and the rest of the world.

Keywords:

Fir, Molecular Taxonomy, Chloroplast DNA Region,

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