Thymus Praecox’un Kimyasal Bileşimi, Antibakteriyel ve Antioksidan Aktiviteleri

Çalışmanın amacı: Kastamonu bölgesinden toplanan Thymus praecox'un kimyasal bileşimini, antioksidan kapasitesini ve antibakteriyel aktivitesini belirlemektir. Materyal ve yöntem: Kimyasal bileşimin belirlenmesinde spektroskopik ve kromatografik analizler uygulandı. Kekik özleri beş farklı çözücü kullanılarak hazırlandı. HPLC kullanılarak Thymus praecox içeriğinde bulunan flavonoidlerin varlığı ve GC-MS kullanılarak uçucu yağın kimyasal içeriği araştırıldı. Kekiğin yaprak ve sapındaki mineral analizleri ICP-OES ile belirlendi. Antioksidan kapasite, DPPH serbest radikal temizleme yöntemi ve H2O2 süpürme yöntemi gibi iki yöntem kullanılarak belirlendi. On bakteri, Gram (+) ve Gram (-) üzerindeki antibakteriyel aktivite disk difüzyon yöntemi kullanılarak test edildi. Temel sonuçlar: Deneysel sonuçlar, kekik ekstraktlarının bazı bakterilere karşı antibakteriyel aktiviteye sahip olduğunu göstermiştir. Sonuç olarak, en çok etkilenen bakterinin S. aureus olduğu bulundu. İnhibisyon zon çapının 8-12 mm arasında olduğu belirlendi. Çözücülerin antibakteriyel aktivite, antioksidan kapasite, toplam fenolik ve toplam flavonoid miktarları üzerine etkisi gözlendi. Araştırma vurguları: Çalışmalar T. praecox'un doğal bir antimikrobiyal ve antioksidan ajan olarak kullanılma potansiyeline sahip olduğunu ve doğal bir destek tedavisi olarak kullanılabileceğini gösterdi.

Chemical Composition, Antibacterial and Antioxidant Activities of Thymus Praecox

Aim of Study: It was aimed to determine the chemical composition, antioxidant and antibacterial activity of the Thymus praecox which distributed in the Kastamonu region. Material and methods: Spectroscopic and chromotographic analysis were applied in the determination of the chemical composition. Thyme extracts were prepared using five different solvents. In Thymus praecox, the presence of flavonoids using HPLC and the chemical content of essential oil using GC-MS were investigated. The analyses of the mineral were determined in leaf and stem of thyme by ICP-OES. Antioxidant capacity was determined using two methods such as DPPH free radical scavenging and H2O2 scavenging. Antibacterial activity on ten bacteria, Gram (+) and Gram (-) was tested using the disc diffusion method. Main results: Experimental results showed that thyme extracts have antibacterial activities against some bacteria. As a result, it was found that the most affected bacteria was S. aureus. Inhibition zone diameter was determined to be between 8-12 mm. The effect of solvent on antibacterial activity, antioxidant capacity, total phenolic and total flavonoid amounts were observed. Highlights: These results showed that T. praecox has the potential to be used as a natural antimicrobial and antioxidant agent, and can be used as a natural supportive treatment.

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Kastamonu Üniversitesi Orman Fakültesi Dergisi-Cover
  • ISSN: 1303-2399
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2001
  • Yayıncı: Kastamonu Üniversitesi
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