BURAK ÇOBAN, Cengiz SAĞLAM, Nursel ESER, İLKNUR BABAHAN BİRCAN

Studying DNA Interactions of Ni(II) Complexes of Thiosemicarbazone Containing Vic-Dioxime Ligands

Vic-dioxime ligands and their metal complexes are very important for their biological activities and thoroughly studied. In addition, several thiosemicarbazone derivatives were synthesized and their potentials as anti-tumor agents were reported. As well as this, Nickel (II) complexes, among the other metal complexes, have an important proportion in DNA interaction studies. The compounds which have all these features (vic-dioxime, thiosemicarbazone group and Ni(II)) are not evaluated for their interactions with DNA. DNA interactions of Ni(II) complex of thiosemicarbazone containing vic-dioxime ligand will be studied by using UV titration, floresans, and agaroz gel electrophoresis methods for the first time.

Tiyosemikarbazon İçeren Vis-Dioksim Ligandının Ni(II) Kompleksinin DNA Etkileşimlerinin İncelenmesi

Vis-dioksim ligantları biyolojik aktiviteleri açısından çok önemlidir ve metal kompleksleri yoğun bir şekilde çalışılmıştır. Ayrıca birçok tiyosemikarbazon türevi de sentezlenmiş ve potansiyel anti-tümör ajanları olarak rapor edilmiştir. DNA’ya bağlanma çalışmalarında kullanılan çeşitli metal kompleksleri arasında Nikel (II) kompleksleri de yer almaktadır. Bütün bu özellikleri (visdioksim, tiyosemikarbazon grubu ve Ni(II)) bir arada bulunduran bileşikler literatürde DNA’ya bağlanması açısından daha evvel hiç değerlendirilmemiştir. Tiyosemikarbazon içeren vis-dioksim ligandının Ni(II) kompleksinin DNA ile etkileşimleri UV titrasyon yöntemi, flouresans, ve agaroz jel elektroforez yöntemleri ile ilk defa çalışılmıştır.

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