Şükran ÖZTÜRK, Yasin HAZER, BANU KAŞKATEPE, MUHAMMET ÖREN

Bazı Yosun Türlerinin Toplam Fenol İçeriklerinin, Antibakteriyel ve Antioksidan Aktivitelerinin Belirlenmesi

Bu çalışmanın amacı Cratoneuron filicinum (Hedw.) Ladin, Campyliadelphus chrysophylus (Brid.) R.S. Chopra. Plasteurhynchium striatum (Spruce) M. Fleisch, Palamocladium euchloron (Bruch ex Müll. Hal.) Wijk & Margad gibi briyofit türlerinin toplam fenol içeriklerini, antibakteriyel ve antioksidan aktivitelerini belirlemektir. Bu türlerin metanolik, etanolik, kloroform, aseton ve su ekstraktlarının antibakteriyel aktiviteleri mikrodilüsyon yöntemi ile, antioksidan aktivitesi 2,2-difenil-1-pikrilhidrazil serbest radikal süpürme yeteneği ile, Toplam fenol içerikleri ise standart olarak gallik asit eşdeğerleri ile folin ciocalteu reaktifi kullanılarak nicel olarak belirlenmiştir. En yüksek DPPH serbest radikal süpürme aktivitesi yaklaşık %65 ile C. filicinum’da gözlendi. P. euchloron’un toplam fenolik içeriği en yüksek ekstre değeri 0,027±0,002 mg/g olarak belirlenmiştir. Briyofitlerin metanol ekstraktının önemli antioksidan aktiviteye ve fenolik içeriğe sahip olduğu bulundu. Ekstraktların test edilen mikroorganizmalara karşı antibakteriyel etkisi gözlenmedi.

Anahtar Kelimeler:

Antimikrobiyal aktivite, Antioksidan aktivite, Briyofitler, Toplam fenol içeriği

Determination of Total Phenol Contents, Antibacterial and Antioxidant Activity of Some Mosses Species

The aim of this study were to determine total phenol contents, antibacterial and antioxidant activity of bryophyte species including Plasteurhynchium striatum (Spruce) M. Fleisch, Palamocladium euchloron (Bruch ex Müll. Hal.) Wijk & Margad. and Cratoneuron filicinum (Hedw.) Spruce and Campyliadelphus chrysophylus (Brid.) R.S. Chopra. Antibacterial activity of methanolic, ethanolic, chloroform, acetone and water extracts of these species was determined by microdilution method. Antioxidant activity of these species was evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging ability. The total phenol content of these species was determined quantitatively using the folin ciocalteu reagent, with gallic acid equivalents as the standard. The highest DPPH free radical scavenging activity was observed in about 65% C. filicinum. Total phenolic content of P. euchloron was determined the highest extract value of 0,027±0,002 mg/g. The methanol extract of bryophytes was found to have significant antioxidant activity and phenolic contents. Antibacterial effect of the extracts was not observed against the tested microorganisms

Keywords:

Antimicrobial activity, Antioxidant activity, Bryophytes, Total phenol contents,

PDF

___

Abdille, H., Singh, RP., Jayaprakasha, GK., Jena, BS. 2005. Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem., 90:891-896. Doi: 10.1016/j.foodchem.2004.09.002.
Bhattarai, HD., Paudel, B., Lee, HK., Joung, H., Yima, H. 2009. In vitro antioxidant capacities of two benzonaphthoxanthenones: Ohioensins F and G isolated from the antarctic moss Polytrichastrum alpinum. Z. Naturforsch., 64:197-200. Doi: 10.1515/znc-2009-3-408.
Bodade, RG., Borkar, PS., Arfeen, MS., Khobragade, CN. 2008. In vitro screening of bryophytes for antimicrobial activity. Journal of Medicinal Plants., 7:23-28.
Chobota, V., Kubicova, L., Nabbout, S., Jahodar, L., Hadacek, F. 2008. Evaluation of antioxidant activity of some common mosses. Z Naturforsch C J Biosci., 63. 476-482. Doi: 10.1515/znc-2008-7-802.
Cui, Y., Kim, DS., Park, KC. 2005. Antioxidant effect of Inonotus obliquus. J. Ethnopharmacol., 96:79-85. Doi: 10.1016/j.jep.2004.08.037.
Cvetić, T., Sabovljević, A., Pristov, JB., Sabovljević, M. 2009. Effects of day length on photosynthetic pigments and antioxidative metabolism of in vitro cultured moss Atrichum undulatum Hedw. P. Beauv. (Bryophyta). Bot Serb., 33(1):83-88.
Das, NP., Pereira, TA. 1990. Effects of flavonoids on thermal autooxidation of palm oil: structure–activity relationship. J. Am. Oil Chem. Soc., 67:255-258.
De Gaulejac, NSC., Glories, Y., Vivas, N. 1999. Free radical scavenging effect of anthocyanins in red wines. Food Res. Int., 32:327-333. Doi: 10.1016/S0963-9969(99)00093-9.
Ellnain-Wojtaszek, M., Kruczynski, Z., Kasprzak, J. 2003. Investigation of the free radical scavenging activitiy of Ginkgo biloba L. leaves. Fitoterapia, 74:1-6. Doi: 10.1016/s0367-326x(02)00306-4.
Glime, JM. 2007. Economic and ethnic uses of bryophytes. In flora of North America Editorial Committee, ed. Flora of North America, North of Mexico. Bryophyta 27:14-41.
Goffinet, B., Shaw, AJ. 2009. Bryophyte biology, 2nd ed., Cambridge University Press, The Edinburgh Building, Cambridge CB2 8RU, UK, 565. Ann. Bot., 104:vi–x. Doi: 10.1093/aob/mcp109.
Hinneburg, I., Dorman, HJG., Hiltunen, R. 2006. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem., 97:122-129. Doi: 10.1016/j.foodchem.2005.03.028.
Hsiao, G., Teng, CM., Wu, CL., Ko, FN. 1996. Marchantin H as a natural antioxidant and free radical scavenger. Arch. Biochem. Biophys., 334(1):18-26. Doi: 10.1006/abbi.1996.0424.
International Organization for Standardization. 2006. ISO 20776-1: Clinical laboratory testing and in vitro diagnostic test systems - Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices -Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.
Jeong, SI., Kim, HS., Jeon, IH., Kang, HJ., Mok, JY., Cheon, CJ., Yu, HH., Jang, SI. 2014. Antioxidant and anti-inflammatory effects of ethanol extracts from Perilla frutescens. Korean J Food Sci Technol., 46:87-93. Doi: 10.9721/KJFST.2014.46.1.87.
Kumar, J., Dhar, P., Tayade, AB., Gupta, D., Chaurasia, OP., Upreti, DK., Arora, R., Srivastava, RB. 2014. Antioxidant Capacities, Phenolic Profile and Cytotoxic Effects of Saxicolous Lichens from Trans-Himalayan Cold Desert of Ladakh. PloS One., 9:6. Doi: 10.1371/journal.pone.0098696.
Magtoto, LM., Alonzo, EAM., Lacerna, MZT., Santiago, JC. 2015. Total Phenol Content and Antioxidant Activity of Bryum billardieri Schwaegr. IJCPR., 7(1):19-20.
Mainasara, MM., Abu Bakar, MF., Md Akim, A., Linatoc, AC., Abu Bakar, FI., Ranneh, YKH. 2021. Secondary Metabolites, Antioxidant, and Antiproliferative Activities of Dioscorea bulbifera Leaf Collected from Endau Rompin, Johor, Malaysia. Based Complement Alternat Med., 2021:8826986. Doi: 10.1155/2021/8826986.
Mishra, K., Ojha, H., Chaudhury, NK. 2012. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chem., 130:1036-1043. Doi: 10.1016/j.foodchem.2011.07.127.
Pejin, B., Bogdanović-Pristov, J. 2012. ABTS cation scavenging activity and total phenolic content of three moss species. Hem Ind., 66(5):723-726. Doi: 10.2298/hemind120131022p.
Pejin, B., Bogdanovic-Pristov, J., Pejin, I., Sabovljevic, M. 2013. Potential antioxidant activity of the moss Bryum moravicum. Nat. Prod. Res., 27(10):900-902. Doi: 10.1080/14786419.2012.665915.
Pejin, B., Savic, A., Kien-Thai, Y., Mojovic, M. 2014. Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy. Cryptogamie Bryol., 35(2):173-179. Doi: 10.7872/cryb.v35.iss2.2014.173.
Rice-Evans, CA., Miller, NJ., Bolwell, PG., Bramley, PM., Pridham, JB. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res., 22:375-383. Doi: 10.3109/10715769509145649.
Sabovljević, M., Bijelović, A., Grubišić, D. 2001. Bryophytes as a potential source of medicinal compounds. Lek. Sirov., 21:17-29.
Sabovljević, A., Vujicic, M., Skoric, M., Bajic-Ljubicic, J., Sabovljević, M. 2012. Axenically culturing the bryophytes: Establishment and propagation of the pleurocarpous moss Thamnobryum alopecurum Nieuwland ex Gangulee (Bryophyta, Neckeraceae) in in vitro conditions. Pak. J. Bot., 44(1):339-344.
Shai, LJ., Masoko, P., Mokgotho, MP., Magano, SR., Mogale, AM., Boaduo, N., Eloff, JN. 2010. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa. S. Afr. J. Bot., 76:465-470. Doi: 10.1016/j.sajb.2010.03.002.
Singleton, VL., Rossi, JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16:144-158.
Singleton, V., Orthofer, R., Lamuela-Ravento´s, R. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In L. Packer (Ed.). Oxidants and antioxidants, part A. Meth. Enzymol., 299: pp. 152–178. New York: Academic Press. Doi: 10.1016/S0076-6879(99)99017-1.
Tunalıer, Z., Öztürk, N., Koşar, M., Başer, KHC., Duman, H., Kırımer, N. 2002: Bazı Sideritis türlerinin antioksidan etki ve fenolik bileşikler yönünden incelenmesi. Bitkisel İlaç Hammaddeleri Toplantısı, s. 76-93, Eskişehir.
Umamaheswari, M., Chatterjee, TK. 2008. In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. Afr. J. Trad. Compl. Altern. Med., 5:61-73.
Velioglu, YS., Mazza, G., Gao, L., Oomah, BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agri. Food Chem. 46:4113-4117. Doi: 10.1021/jf9801973.
Yağlıoğlu, MS., Abay, G., Demirtaş, I., Yağlıoğlu, AS. 2017. Phytochemical screening, antiproliferative and cytotoxic activities of the mosses Rhytidiadelphus triquetrus (Hedw.) Warnst. and Tortella tortuosa (Hedw.) Limpr. Anatolian Bryol. 3(1):31-42. Doi: 10.26672/anatolianbryology.317785
Wang, H., Cao, G., Prior, RL. 1996. Total antioxidant capacity of fruits. J. Agric. Food Chem., 44:701-705. Doi: 10.1021/jf950579y.