Zinnur GEREK, İSMAİL MALKOÇ, Seçil Nazife PARLAK

The Effect of Estrogen Treatment on Eccentric Exercise-Induced Damage in Rat Kidneys

Bu çalışmanın amacı, anabolik steroidlerin etkilerini eksantrik egzersiz uygulanan sıçanların böbrek dokusunda, biyokimyasal ve histolojik yöntemler kullanarak incelemektir. Sprague Dawley erkek fareler (n = 36), eşit şekilde östrojen uygulanan (17-? Estradiol, günde 10 mg/kg) ve östrojen uygulanmayan iki gruba ayrıldı. Bu iki grup da kendi aralarında eksantrik egzersiz uygulanan (n = 12) ve uygulanmayan (n = 6) olmak üzere iki alt gruba ayrıldı. Daha sonra eksantrik egzersiz uygulanan gruplarda egsersiz işleminde bir saat (n = 6) ve 48 saat (n = 6) sonra dekapite edilerek iki alt gruba ayrıldı. Östrojenin eksantrik egzersiz uygulanan sıçanların böbrek dokusunda histopatolojik etkileri olduğu görüldü. Östrojen ve östrojen+eksantrik egzersiz uygulanan grupların böbrek dokularında hemoraji, damarlarda endotel bağımlı vazodilatasyonlar ve proksimal tübüller, glomerüller ve bağ dokusunda da dejeneratif değişiklikler saptandı. Eksantrik egzersiz ve eksantrik egzersiz+östrojen uygulanan gruplarda distal tübüllerde dejenerasyonlar ve dilatasyonlar, peritübüler kapiller dilatasyonları ve bağ dokuya polimorfonükleer lökosit (PMNL) sızıntıların saptandı. Bununla birlikte östrojen uygulanan gruplarda PMNL infiltrasyonunda dikkat çekici bir azalma izlendi.. Biyokimyasal sonuçlar Kontrol grubu dışındaki diğer deneysel gruplarda oksidatif stress olduğunu gösterdi. Östrojenin eksantrik egzersize bağlı böbrek hasarı önlemede anti-inflamatuar etkisinin görülmesine rağmen yeterli derecede etkin olamayabileceği ve klinik uygulamalarda böbrek dokuları üzerinde toksik etkileri olabileceği sonucuna varılmıştır. Bu alanda ileriye yönelik daha ayrıntılı çalışmalar yapılabilir

Östrojen Tedavisinin Eksantrik Egzersiz Uygulanan Sıçan Böbrekleri Üzerinde Oluşturduğu Hasara Etkisi

The aim of this study was to examine the effects of anabolic steroids on eccentric exercise-induced kidney tissue, using biochemical and histological methods. Sprague Dawley male rats (n=36) were equally divided into the estrogen (17-? Estradiol, 10 mg/kg per day)induced and non estrogen-induced groups. Both groups were subdivided into as rest groups (n=6), and as exposed to the eccentric exercise groups (n=12) that were also subdivided into decapitated one hour (n=6) and 48 hour (n=6) groups after eccentric exercise. Histopathological effects of estrogen treatment on eccentric exercise-induced rat kidney tissues were seen. The histopathological results revealed that hemorrhage, endothelium-dependent vasodilatations and degenerative changes in proximal tubules, glomeruli, and connective tissue in the estrogen-given and estrogen-given plus eccentric exercise groups. Distal tubule degenerations and dilatations with peritubular capillary dilatations and polymorphonuclear leucocyte (PMNL) infiltrations to connective tissue in eccentric exercise and eccentric exercise plus estrogen-given groups were detected but also it was noticeable decreases in PMNL infiltrations in estrogen-given groups. Biochemical results showed oxidative stress in experimental groups except Control group. It has been concluded that estrogen could not have been effective for preventing eccentric exercise-induced kidney damage and might have toxic effects on kidney tissues on clinical practice however anti-inflammatory effect on kidney tissue detected. It has been requested further investigation

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