DİDEM PEKMEZCİ, SENA ÇENESİZ, Duygu ÇAKIROĞLU, GÜLAY ÇİFTCİ, Ahmet ÇIRA, GÜVENÇ GÖKALP

Status of lipid peroxidation, cell destruction and the antioxidant capacity in foals with lower respiratory tract disease

Bu çalışma ile alt solunum yolu hastalıkları şekillenen taylarda lipit peroksidasyon düzeyini belirlemek amacı ile serum malondialdehit (MDA), hücresel hasar seviyesi için serum siyalik asit (SA) ve serum antioksidan kapasiteleri için serum seruloplazmin (CP), serum ürik asit (UA) ve serum albumin seviyelerinin değerlendirilmesi amaçlanmıştır. Çalışmada deney grubunu yaşları 5.5±1.5 aylık olan alt solunum yolu hastalığı şekillenen 8 adet tay oluştururken, kontrol grubunu ise 8 adet sağlıklı tay oluşturmuştur. Çalışma ve kontrol grupları arasında serum MDA, CP, SA ve UA seviyeleri bakımından istatiksel olarak farklılık (P

Anahtar Kelimeler:

antioksidanlar, biyokimyasal belirleyiciler, seruloplazmin, teşhis, tanı teknikleri, tanı değeri, tay, at, lipit peroksidasyonu, lipid peroksit, malonaldehit, solunum yolu hastalıkları, solunum sistemi, serum albumin, siyalik asit, ürik asit

Alt solunum yolu hastalıkları olan taylarda lipit peroksidasyon, hücresel hasar ve antioksidan kapasite durumları

The aim of this study was to demonstrate the total serum malondialdehyde (MDA) levels for lipid peroxidation status, total serum sialic acid (SA) levels for cell destruction level, serum ceruloplasmin (CP), albumin and uric acid (UA) for the antioxidant capacity in foals with lower respiratory tract disease. The material of the study was consisted of 8 foals as the study group, with lower respiratory tract disease and 8 healthy foals as the control group, aged between 5.5±1.5 months. The results revealed a significant difference (P<0.05), between the groups for serum MDA, CP, SA and UA levels, but a difference had not been determined for serum albumin level. In conclusion, it was determined that serum MDA, SA, CP and UA levels may be useful parameters as an indicator of lipid peroxidation, cell destruction and antioxidant capacity in foals with lower respiratory tract disease.

Keywords:

antioxidants, biochemical markers, ceruloplasmin, diagnosis, diagnostic techniques, diagnostic value, foals, horses, lipid peroxidation, lipid peroxides, malonaldehyde, respiratory diseases, respiratory system, serum albumin, sialic acids, uric acid,

PDF

___

1. Wilson WD: Foal pneumonia: An overview. Proceedings of the 38th Annu Conv AAEP, pp. 203-229, 1992.
2. Cohen ND: Causes of and farm management factors associated with disease and death in foals. J Am Vet Med Assoc, 204, 1644-1651, 1994.
3. Meram I, Aktaran S: Serbest radikallerin biyomoleküller üzerine etkileri. Arşiv, 11, 299-304, 2002.
4. Lunec J, Halloran SP, White AG, Dormandy TL: Free radical oxidation (peroxidation) products in serum and synovial fluid in rheumatoid arthritis. J Rheumatol, 8, 233-245, 1981.
5. Winrow VR, Winyard PG, Morris CJ, Blake DR: Free radicals in inflammation: second messengers and mediators of tissue destruction. Br Med Bull, 49, 506-522, 1993.
6. Halliwel B, Chrica S: Lipid peroxidation. Its mechanism measurements and significance. Am J Clin Nutr, 57 (Suppl.): 715-725, 1999.
7. Jacobsen S, Andersen PH. The acute phase protein serum amyloid A (SAA) as a marker of inflammation in horses. Equine Vet Edu, 19, 38-46, 2007.
8. Okumura M, Asano M, Tagami M, Tsukiyama K, Fujinaga T: Serum copper and ceruloplasmin activity at the early growing stage in foals. Can J Vet Res, 62, 122-126, 1998.
9. Karapehlivan M, Atakisi E, Citil M, Kankavi O, Atakisi O: Serum sialic acid levels in calves with pneumonia. Vet Res Comm, 31, 37-41, 2007.
10. Yurtseven S, Uysal H: Decreased serum sialic acid, albumin-globulin ratio and total protein levels in cattle heavily infected with Theileria annulata. Ankara Üniv Vet Fak Derg, 56, 141-144, 2009.
11. Moshage HJ, Janssen JAM, Franssen JH, Hafkenscheid JCM, Yap SH: Study of the molecular mechanism of decreased liver synthesis of albumin in inflammation. J Clin Invest, 79, 1635-1641, 1987.
12. MacKinnon KL, Molnar Z, Lowe D, Watson ID, Shearer E: Measures of total free radical activity in critically ill patients. Clin Biochem 32, 263-268, 1999.
13. Yoshioka T, Kawada K, Shimada T, Mori M: Lipid peroxidation in maternal and cord blood and protective mechanism aganist actived oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376, 1979.
14. Warren L: The thiobarbituric acid assay of sialic acids. J Biol Chem, 234, 1971-1975, 1959.
15. John PWM: Statistical Design and Analysis of Experiments (Classics in Applied Mathematics No 22.) Society for Industrial Mathematics. 1999.
16. Lykkesfeldt J, Svendsen O: Oxidants and antioxidants in disease: Oxidative stress in farm animals. Vet J, 173, 502-511. 2007.
17. Wessely-Szponder J, Bobowiec R, Martelli F, Wojcik M, Kosior-Korzecka U: Assessment of neutrophil components as markers of lung injury in the course of bovine respiratory tract infections, Pol J Vet Sci, 7, 157-161. 2004.
18. Lauritzen B, Lykkesfeldt J, Friis C: Evaluation of a single dose versus a divided dose regimen of amoxicillin in treatment of Actinobacillus pleuro-pneumoniae infection in pigs. Res Vet Sci, 79, 61-67, 2005.
19. White A, Estrada M, Walker K, Wisnia P, Filgueira G, Valdes F, Araneda O, Behn C, Martinez R: Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comp Biochem Physiol A, 128, 99-104, 2001.
20. Kızıl O, Kızıl M: Ürtiker semptomlu atlarda plazma alkalen fosfataz, aspartat amino transferaz, laktat dehidrogenaz, gama glutamil transferaz, total protein, albumin ve lipid peroksidasyon düzeyleri. FÜ Sağ Bil Vet Derg, 20, 167-172, 2006.
21. Kızıl O, Kızıl M, Yüce A: Sancı semptomlu atlarda plazma oksidatif stress parametreleri. Kafkas Univ Vet Fak Derg, 12 (1): 37-40, 2006.
22. Gündoğdu S, Ertekin A: İnsanlarda üst ve alt solunum yolu enfeksiyon-larının lipit peroksidasyonu, antioksidan vitaminler ve antioksidan savunma sistemleri üzerine etkilerinin araştırılması. YYÜ Vet Fak Derg, 17, 19-25, 2006.
23. Li GF, He YF, Huang MR, Guo JL, Wu QT, Chen SJ: Determination of serum superoxide dismutase, glutathione peroxidase, total antioxidative capacity, malondialdehyde in patients with pneumonia. Di Yi Jun Yi Da Xue Xue Bao, 23, 961-962, 2003.
24. Deaton CM, Marlin DJ, Smith NC, Harris PA, Dagleish MP, Schroter RC, Kelly F: Effect of acute airway inflammation on the pulmonary antioxidant status. Exp Lung Res, 7, 653-670, 2005.
25. Art T, Kirschvink N, Smith N, Lekeux P: Indices of oxidative stress in blood and pulmonary epithelium lining fluid in horses suffering from recurrent airway obstruction. Equine Vet J, 31, 397-401, 1999.
26. Okumura M, Fujinaga T, Yamashita K: Isolation, characterization, and quantitative analysis of ceruloplasmin from horses. Am J Vet Res 52, 1979-1985, 1991.
27. Smith JE, Cipriano JE: Inflammation-induced changes in serum iron analytes and ceruloplasmin in Shetland ponies. Vet Pathol, 24, 354-356, 1987.
28. Sobol G, Pyda E: Copper and ceruloplasmin concentrations in serum of infants with pneumonia. Pneumonol Alergol Pol, 63, 378-381, 1995
29. Pfeffer A, Rogers KM: Acute phase response of sheep: Changes in the concentrations of ceruloplasmin, fibrinogen, haptoglobin and the major blood cell types associated with pulmonary damage. Res Vet Sci, 46, 118-24, 1989.
30. Van Tongeren JHM, Cluysenaar OJJ, Lamers CBH, de Mulder PHM, Yap SH: Causes of hypoalbuminaemia. In, Yap SH, Majoor CLH, Van Tongeren JHM (Eds): Clinical Aspects of Albumin. pp. 117-133, Martinus Nijhoff Publishing Co., The Hague, Netherlands, 1978.
31. Vengust M, Stæmpfli H, Prescott JF: Rhodococcus equi pleuropneumonia in an adult horse. Can Vet J, 43 (9): 706-708, 2002.
32. King, LG: That dog still has pneumonia? How to approach tough cases of bacterial pneumonia in dogs. Proceeding of the SEVC, Southern European Veterinary Conference, Oct. 2-4, Barcelona, Spain, 2009.
33.Anker SD, Doehner W, Rauchhaus M, Sharma R, Francis D, Knosalla C, Davos CH, Cicoira M, Shamim W, Kemp M, Segal R, Osterziel KJ, Leyva F, Hetzer R, Ponikowski P, Coats AJ: Uric acid and survival in chronic heart failure: validation and application in metabolic, functional, and hemodynamic staging. Circulation 107, 1991-1997, 2003.
34. Mair TS: Bacterial pneumonia associated with corticosteroid therapy in three horses. Vet Rec, 138 (9): 205-207, 1996.
35. Kirschvink N, Fiévez L, Bougnet V, Art T, Degand G, Smith N, Marlin D, Roberts C, Harris P, Lekeux P: Effect of nutritional antioxidant supplementation on systemic and pulmonary antioxidant status, airway inflammation and lung function in heaves-affected horses. Equine Vet J, 34, 705-712, 2010.
36. McCuller JA, Bartmess KC: Role of neuraminidase in lethal synergism between influenza virus and Streptococcus pneumoniae. J Infect Dis, 187, 1000-1009, 2003.