Isolation of Citrobacter freundii from Rainbow Trout (Oncorhynchus mykiss) in Freshwater Cage

In this study, the bacteriological examination was done in case of disease suspect of rainbow trout (Oncorhynchus mykiss) which kept in cages in the Black Sea Region of Turkey. The bacterial agent was identified as Citrobacter braakii by rapid test kit (API 20E, Profile: 1704553), further identification was performed by 16S rRNA gene sequencing. Bacteria were identified as Citrobacter freundii by further molecular analysis. The antibacterial susceptibility of bacteria was also determined for 6 different antibiotics. The bacteria was sensitive to florfenicol, enrofloxacin, oxytetracycline and trimethoprim+sulfamethoxazole and resistant to erythromycin and amoxicillin-clavulanate. The most effective antibiotic was florfenicol. The disease was treated with florfenicol.

Tatlısu Üzerindeki Kafeslerde Üretilen Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Citrobacter freundii İzolasyonu

Bu çalışmada, Türkiye’nin Karadeniz Bölgesi'nde kafeslerde yetiştirilen gökkuşağı alabalıkları (Oncorhynchus mykiss) hastalık şüphesi sonrası bakteriyolojik olarak araştırılmıştır. Balıklardan izole edilen bakteriyel etken, hızlı teşhis kiti ile Citrobacter braakii olarak isimlendirilmiştir (API 20E, Profil: 1704553). Bakterinin kesin tanımlanması ise 16S rRNA gen bölgesinin sekansı ile gerçekleştirilmiştir ve bakteri Citrobacter freundii olarak tanımlanmıştır. Ayrıca bakterinin altı farklı antimikrobiyale karşı antibakteriyel hassasiyeti belirlenmiştir. Bakteri florfenikol, enrofloksasin, oksitetrasiklin ve trimethoprim-sulfamethaksazol antibiyotiklerine karşı duyarlı, eritromisin ve amoksisilin antibiyotiklerine karşı ise dirençli olarak bulunmuştur. En etklili antibiyotik florfenikoldür. Hastalık florfenikol ile tedavi edilmiştir.

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