Farklı Benzil Amino Pürin ve Gibberellik Asit Kombinasyonunun Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Mikroçoğaltımı Üzerine Etkisi

Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne geneneksel tıp sisteminde hastalıkların tedavisinde kullanılan önemli akuatik tıbbi bitkidir. Bu çalışmada, 6-benzil amino purin (BAP) ve gibberellik asit (GA3)’in farklı kombinasyonlarını içeren kültür ortamında R. rotundifolia’nın boğum eksplnatlarından in vitro sürgün rejenerasyonu araştırılmıştır. Kültür ortamlarında ilk sürgün oluşumları 10 gün sonunda gözlenmiştir. Sekiz hafta sonunda deneme sonlandırılmış ve rejenerasyon verileri alınmıştır. Sürgün rejenerasyon oranı %72,22-100,00 arasında sıralanmıştır. %100 sürgün rejenerasyon oranı 0,25-0,75 mg/L BAP + 0,25 mg/L GA3 içeren MS ortamında kaydedilmiştir. Eksplant başına maksimum sürgünler (18,38 adet) ve en uzun sürgünler (2,36 cm) 0,25 mg/L BAP + 0,25 mg/L GA3 eklenmiş kültürlerde elde edilmiştir. Çoğaltım ortamında sürgünlerin köklenmesi nedeniyle ayrıca köklendirme çalışması yürütülmemiştir. Rejenere sürgünlerin akvaryum ortamına alıştırılması başarıyla sağlanmıştır. Bu rapor, tıbbi bitki R. rotundifolia’nın çoklu üretimini sunması bakımından önemlidir. İleride bu bitki ile yürütülecek sekonder metabolit üretimi ve gen aktarım çalışmalarına yardımcı olabilir.

Anahtar Kelimeler:

Boğum eksplantı, Doku kültürü, Sürgün rejenerasyonu, BAP

The Effect of Different Combinations of Benzyl Amino Purine and Gibberellic Acid on Micropropagation of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne is an important aquatic medicinal plant for the treatment of diseases in the traditional medicine system. In this study, in vitro shoot regeneration from the nodal explants of R. rotundifolia in culture medium containing different combinations of 6-benzyl amino purine (BAP) and gibberellic acid (GA3) was investigated. First shoot formation was observed after 10 days in culture medium. At the end of eight weeks, the trial was completed and regeneration data were obtained. Shoot regeneration rate is listed as 72.22-100.00%. 100% shoot regeneration rate was recorded in MS medium including 0.25-0.75 mg/L BAP + 0.25 mg/L GA3. The maximum shoots per explant (18.38) and the longest shoots (2.36 cm) were obtained in cultures with 0.25 mg/L BAP + 0.25 mg/L GA3. No rooting study was carried out due to the rooting of the shoots in the multiplication environment. The acclimation of regenerated shoots to the aquarium environment has been successfully achieved. This report is important in that it offers multiple production of medicinal plant R. rotundifolia. In the future, it can help with the production of secondary metabolites and gene transfer studies.

Keywords:

Nodal explant, Tissue culture, Shoot regeneration, BAP,

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