Melisa BEYHAN YILMAZ, Fahriye ZEMHERİ NAVRUZ, Ecem SEÇGİNLİ

Drosophila melanogaster’de Antidepresan (Sitalopram) Kullanılarak Oluşturulan Strese Karşı E ve C Vitamininin Koruyucu Etkisi

Bu çalışmada antidepresan etken maddesi olan Sitalopram’ın D. melanogaster ömür uzunluğuna ve eşey oranına olumsuz etkileri ve buna karşı C ve E vitamininin koruyuculuğu araştırıldı. Sitalopram’ın 3 farklı dozu (0-1mM) ve C-E vitaminlerinden (25mg/L) oluşturulan gruplara beslenme yolu ile verildi. Döl sayısı deneyi 7 gün süreyle, ömür uzunluğu deneyi 39 gün süreyle uygulandı. Bu çalışmada yer verilen döl sayısı deneylerinde, uygulama gruplarında döl veriminin azalması beklenirken, erişkin sineklerin oluşturduğu döl sayısının kontrol grubu ile yakın sayılarda olduğu gözlemlendi. Ayrıca vitaminli gruplarda ise döl sayısı kontrole kıyasla düşüşe geçti. Bununla birlikte ömür uzunluğu deneyinde kontrol grubunda hayatta kalma yüzdesi %80’lere düşerken Sitalopram’lı gruplarda hayatta kalma yüzdesi %80’nin üzerinde kaldı. Ancak Sitalopram ve vitaminlerin birlikte uygulandığı grupların hayatta kalma oranları %60’lara inerek kontrol grubuna göre düşük seyretti. Sonuç olarak; Sitalopram’ın sineklerin döl sayısını değiştirmediği, ömür uzunluğu deneylerinde ise vitamin ile birlikte alınan ilacın kontrol grubuna kıyasla ömrü olumsuz etkilediği belirlendi.

Anahtar Kelimeler:

Sitalopram, C vitamini, E vitamini, yavru döl sayısı, ömür uzunluğu

Protective Effect of Vitamins E and C Against Stress Generated Using Antidepressant (Citalopram) in Drosophila melanogaster

In the study, the negative effects of Citalopram, which is an antidepressant active ingredient, on D. melanogaster lifespan and sex ratio, and the protection of vitamins C and E against this were investigated. Three different doses of citalopram (0-1mM) and vitamins C and E (25mg/L) were given to the groups by diet. The number of progeny tests was carried out for 7 days, and the longevity test was performed for 39 days. In the progeny number experiments included in this study, it was observed that while the fertility of the treatment groups was expected to decrease, the number of offspring formed by adult flies was close to the control group. In addition, the number of offspring in the vitamin group decreased compared to the control. However, in the longevity experiment, the survival rate in the control group decreased to 80%, while the survival rate in the Citalopram groups remained above 80%. However, the survival rate of the groups in which citalopram and vitamins were administered together was lower than the control group, decreasing to 60%. As a result; It was determined that citalopram did not change the number of offspring of the flies, and in the longevity experiments, the drug taken together with the vitamin affected the lifespan negatively compared to the control group.

Keywords:

citalopram, vitamin E, vitamin C, number of spring, longevity,

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