Karpuzun (Citrullus Lanatus) Dış ve İç Kabuğundaki Antioksidanların Koruyucu Etkisi

İnsan sağlığına faydalı olduğu bilinen karpuz (Citrullus lanatus) yaygın olarak tüketilmektedir. Çalışma, C. lanatus’un dış ve iç kabuklarının fitokimyasal bileşenleri, serbest radikal temizleme aktiviteleri ve besleyici içeriğini değerlendirmek amacıyla yapılmıştır. Bu amaçla, çalışmada karpuzun dış ve iç kabuklarından elde edilen etanol ekstraktında toplam flavonoid / fenolik içeriği ve antioksidan aktivitesi belirlendi. Antioksidan aktivite, CUPRAC, DPPH, FRAP ve potasyum tiyosiyanat yöntemi gibi in vitro deneysel yöntemle belirlenmiştir. C. lanatus dış ve iç kabuğundaki toplam fenolik ve flavonoid içeriği sırasıyla 40.5 ve 32.4 mg GAE g-1 ve 12.6 and 5.8 mg QE g -1 olarak bulunmuştur. C. lanatus dış ve iç kabuğundan elde edilen etanol ektrakt konsantrasyonu (20 μg/ml), aynı konsantrasyondaki a-tokoferolle (% 85.3) karşılaştırıldığında maksimum lipit peraksidasyonunda % 73.2 ve % 72.0 oranında inhibisyon sağladı. Sonuç olarak, dış ve iç kabuklar metal indirgeme kapasitesi ve orta derecede serbest radikal giderme aktivitesine sahip olup ve aynı zamanda lipid peroksidasyon sürecini inhibe eder. Bu nedenle, C. Lanatus'un özellikle dış kabuğu, radikal süpürücüler gibi davranan, oksidasyonu inhibe eden veya geciktiren ve lipid peroksidasyonunu geciktirerek besin maddelerinin raf ömrünü uzatan doğal bir bileşene sahip olabilir.

Anahtar Kelimeler:

Radical giderme aktivitesi, oksidatif stres, oksidatif stres, citrullus lanatus, antioksidan

Effect of Antioxidants as Preservatives in the Outer and Inner Shells of Watermelon (Citrullus Lanatus)

Watermelons (Citrullus lanatus) that are known to be beneficial to human health are widely consumed. The study was performed out to investigate the phytochemical components, free radical scavenging activities and nutritive contents of the outer and inner shells of C. lanatus. For this purpose, the study was determined the total flavonoid/phenolic content and antioxidant activity of ethanol extract prepared from outer and inner shells. The antioxidant activity was determined by in vitro experimental method, such as CUPRAC, DPPH, FRAP and potassium thiocyanate method. Total phenolic and flavonoid contents in outer and inner shell of C. lanatus were found to be 40.5 and 32.4 mg GAE g-1 and 12.6 and 5.8 mg QE g-1, respectively. The concentration of ethanol extract obtained from C. Lanatus outer and inner shell (20 μg/mL) has inhibition of maximum lipid peroxidation in rate % 73.2 and % 72.0 respectively compared to that of α-Tokoferol (% 85.3) at the same concentration. As a result, the outer and inner shells have metal reduction capacity and moderate free radical removal activity and also inhibited the lipid peroxidation process. Therefore, C. Lanatus's especially the outer shell may have a natural ingredient that acts as radical scavengers, inhibits or delays the oxidation and prolongs the shelf life of nutrients by delaying the lipid peroxidation.

Keywords:

Radical cavenging activity, oxidative stress, citrullus lanatus, antioxidant,

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