Karbontetraklorür’ün Neden Olduğu Sıçan Böbrek Dokusu Hasarlarına Karşı Kuersetin’in Koruyucu Etkisi

Bu çalışmada, sıçan böbrek dokusunda karbontetraklorür’ün neden olduğu hasara karşı kuersetin’in etkisi araştırıldı. Çalışmada ağırlıkları yaklaşık (200-300) gr ağırlığında 28 adet yetişkin wistar albino sıçan kullanıldı ve dört gruba ayrıldı. 3 günlük uygulamayı takiben sıçanlar anestezi altına alınarak böbrek dokuları alındı. Böbrek dokusunda SOD, CAT, GPx enzim aktiviteleri ve (8-OHdG) düzeyi değerlendirildi. SOD, CAT, GPx enzim aktiviteleri değerlendirildiğinde, kontrol grubuna oranla CCl4 uygulanan grupta enzim aktivitesinde azalış, CCl4 grubuna oranla CCl4 + kuersetin uygulaması yapılan grupta enzim aktivite düzeyinde artış olduğu belirlendi. Çalışmada, karbontetraklorüre maruz kalan sıçanların böbrek dokusunda 8-OHdG düzeyi değerlendirildiğinde; kontrole oranla karbontetraklorür uygulanan grupta artma, yine kontrole oranla karbontetraklorür + kuersetin uygulanan grupta ise artma, karbontetraklorür grubuna oranla ise karbontetraklorür + kuersetin uygulanan grupta düşme olduğu gözlendi. Kuersetinin, CCl4 tarafından indüklenen böbrek doku hasarı üzerine koruyucu etkisinin olduğu kanaatine varıldı.

Anahtar Kelimeler:

Böbrek hasarı, Karbotetraklorür, Kuersetin, Koruyucu etki, Oksidatif stres

The Protective Effect of Quercetin Against to Renal Tissue Damage Induced Carbontetrachloride in Rat

In this study, the protective effect of quercetin against to the renal tissue damage caused by carbontetrachloride in rat was investigated. It was used 28 adults wistar albino rats weighing approximately 200-300 gr and were divided into four groups. Following 3 days of intraperitoneal application, rats were anesthetized and kidney tissues were taken.As biochemical parameters, SOD, CAT, GPx activities and (8-OHdG) levels were evaluated in renal tissue. When SOD, CAT, GPx enzyme activity was evaluated, decrease in enzyme activity in CCl4 group class with control group and increase in enzyme activity in CCl4 + quercetin group class withCCl4 group was determined.In this study, when 8-OHdG level of kidney tissue exposed to carbontetrachloride was evaluated; In the group treated with carbontetrachloride compared to the control, it was observed that the increase in the group of carbontetrachloride + quercetin compared to the control and the decrease in the group of carbontetrachloride + quercetin compared to the carbontetrachloride group. It was concluded that quercetin had protective effect on CCl4-induced kidney tissue damage.

Keywords:

Renal damage, Carbontetrachloride, Quercetin, Protective effect, Oxidative stress,

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