Ecballium elaterium’un Farklı Kanser Hücre Hatlarına Karşı Sitotoksik Aktivitesi

Bu çalışma kapsamında, halk arasında acı kavun olarak bilinen Ecballium elaterium’un, bazı kanser hücre hatlarına karşı sitotoksik etkilerinin araştırılması hedeflenmiştir. Eski Mısırlılar döneminden beri tanınan bir drog olan E. elaterium birçok hastalığa karşı kullanılmıştır. Bu çalışmada ise son derece saldırgan ve tedavisi bulunmayan malign melanoma (SK-MEL-30), kolon kanseri (CACO-2), meme kanseri (MCF-7) ve küçük hücreli olmayan akciğer kanseri (H1299) hücre hatlarına karşı E. elaterium’un meyve ve yaprak ekstrelerinin sitotoksik etkileri MTT testi ile değerlendirilmiştir. E. elaterium’un meyve ve yaprak ekstreleri aynı zamanda sağlıklı fare fibroblast hücre hattı (L929) üzerinde denenmiştir. Sonuç olarak; E. elaterium’un meyve ve yaprak ekstreleri; SK-MEL-30 hücre hattına karşı zayıf, H1299 hücre hattına karşı orta, MCF-7 hücre hattına karşı yüksek sitotoksik aktivite sergilemiştir. CACO-2 hücre hattına karşı ise her iki ekstrenin de toksik bir etkisi olmadığı gözlemlenmiştir. Ayrıca, meyve ekstrelerinin yaprak ekstrelerinden daha yüksek sitotoksik aktivite sergilediği tespit edilmiştir.

Anahtar Kelimeler:

Ecballium elaterium, meme kanseri, akciğer kanseri, sitotoksik aktivite

Cytotoxic Activity of Ecballium elaterium Against Various Cancer Cell Lines

This study aims to investigate the cytotoxic effects of Ecballium elaterium, known as squirting cucumber or exploding cucumber among the people, against some cancer cell lines. E. elaterium has been used as a drug against many diseases since the ancient Egyptians. The cytotoxic effects of fruits and leaves extracts of E. elaterium against malignant melanoma (SK-MEL-30), colon (CACO-2), breast (MCF-7), and non-small cell lung (H1299) cancerous cell lines were evaluated by the MTT test. Toxicity of the fruits and leaves extracts of E. elaterium were also tested on the mouse fibroblast cell line (L929). As a result, fruits and leaves extracts of E. elaterium showed weak cytotoxic activity against the SK-MEL-30 cell lines, while moderate cytotoxic activity against H1299 cell lines, and high cytotoxic activity against the MCF-7 cell lines. Against the CACO-2 cell line, however, it was observed that both extracts had no cytotoxic effects. Also, fruit extracts exhibited higher cytotoxic activity than leaf extracts.

Keywords:

Ecballium elaterium, Breast cancer, Lung cancer, Cytotoxic activity,

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Arslan D, Ekinci A, Arıcı A, Bozdemir E, Akil E, Özdemir HH, 2017. Effects of Ecballium elaterium on Brain in a Rat Model of Sepsis-Associated Encephalopathy. Libyan Journal of Medicine, 12 (1): 1-6.
Attard E, Cuschieri A, 2004. Cytotoxicity of Cucurbitacin E Extracted from Ecballium elaterium in vitro. Journal of Natural Remedies, 4: 137-144.
Attard E, Brincat MP, Cuschieri A, 2005. Immunomodulatory Activity of Cucurbitacin E Isolated from Ecballium elaterium. Fitoterapia, 76: 439-441.
Baytop T, 1999. Türkiye’de Bitkilerle Tedavi Geçmişte ve Bugün. Nobel Tıp Kitabevleri s. 206-207, İstanbul-Türkiye.
Batistatou A, Cook MG, Massi D, 2009. Histopathology report of cutaneous melanoma and sentinel lymph node in Europe: a web-based survey by the Dermatopathology Working Group of the European Society of Pathology. Virchows Archiv, 454: 505-511.
Baurebaba L, Gilbert-Lopez B, Oukil N, Bedjou F, 2020. Phytochemical composition of Ecballium elaterium extracts with antioxidant and anti-inflammatory activities: Comparison among leaves, flowers and fruits extracts. Arabian Journal of Chemistry, 13: 3286-3300.
Bizid S, Sabbah M, Msakni I, Slimene B, Mohamed G, Bouali R, Abdallah H, Abdelli N, 2014. Cholestatic Hepatitis due to Ecballium elaterium Ingestion. Clinics and Research in Hepatology and Gastroenterology, 39 (5): e61-e63.
Bohlooli S, Jafari N, Jahed S, 2012. Cytotoxic Effect of Freeze-Dried Extract of Ecballium elaterium Fruit on Gastric Adenocarcinoma (AGS) and Esophageal Squamous Cell Carcinoma (KYSE30) Cell Lines. Journal of Gastrointestinal Cancer, 43: 579-583.
Chen X, Bao J, Guo J, Ding Q, Lu J, Huang M, Wang Y, 2012. Biological Activities and Potential Molecular Targets of Cucurbitacins: A Focus on Cancer. Anti-Cancer Drugs, 23: 777-787.
Disse M, Reich H, Lee PK, Schram SS, 2016. A review of the association between parkinson disease and malignant melanoma. Dermatologic Surgery 42: 141-146.
Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, Bedi YS, Taneja SC, Bhat HK, 2008. Medicinal Plants and Cancer Chemoprevention, Current Drug Metabolism, 9 (7): 581-591.
El Sayed ZI, Badr WH, 2012. Cucurbitacin Glucosides and Biological Activities of the Ethyl Acetate Fraction from Ethanolic Extract of Egyptian Ecballium elaterium. Journal of Applied Sciences Research, 8 (2): 1252-1258.
Felhi S, Saoudi M, Daoud A, Hajlaoui H, Ncir M, Chaabane R, El Feki A, Gharallah N, Kadri A, 2017. Investigation of Phytochemical Contents, in vitro Antioxidant and Antibacterial Behavior and in vivo Anti-inflammatory Potential of Ecballium elaterium Ethanol Fruits Extract. Food Science and Technology, 37 (4): 558-563.
Global Canser Observatory (GCO), 2020. Estimated Number of Deaths in 2018, https://gco.iarc.fr/today/online-analysis-table?v=2018&mode=cancer&mode_population=continents&population=900&populations=900&key=asr&sex=0&cancer=39&type=0&statistic=5&prevalence=0&population_group=0&ages_group%5B%5D=0&ages_group%5B%5D=17&group_cancer=1&include_nmsc=1&include_nmsc_other=1 (Erişim Tarihi: 07.07.2020).
Hamidi M, Ghasemi S, Bighdilou BB, Koohi DE, Yousefbeyk F, 2019. Evaluation of Antioxidant, Antibacterial and Cytotoxic Activity of Methanol Extract from Leaves and Fruits of Iranian Squirting Cucumber (Ecballium elaterium (L.) A. Rich). Research Journal of Pharmacognosy 7 (1): 23-29.
Jacquot C, Rousseau B, Carbonnelle D, Chinou I, Malleter M, Tomasoni C, Roussakis C, 2014. Cucurbitacin-D-Induced CDK1 mRNA Up-Regulation Causes Proliferation Arrest of a Non-small Cell Lung Carcinoma Cell Line (NSCLC-N6). Anticancer Research, 34: 4797-4806.
Jafargholizadeh N, Zargar SJ, Aftabi Y, 2018. The Cucurbitacins D, E, and I from Ecballium elaterium (L.) Upregulate the LC3 Gene and Induce Cell-Cycle Arrest in Human Gastric Cancer Cell Line AGS. Iranian Journal of Basic Medical Sciences, 21 (3): 253-259.
Khalil AM, Qaoud KM, 1993. Toxicity and Partial Characterization of Ecballium elaterium Fruit Juice. International Journal of Pharmaceutics, 3: 135-141.
Ma G, Luo W, 1, Lu J, Ma DL, Leung CH, Wang Y, Chen X, 2016. Cucurbitacin E Induces Caspase-Dependent Apoptosis and Protective Autophagy Mediated by ROS in Lung Cancer Cells. Chemico-Biological Interactions, 253: 1-9.
Mazokopakis EE, Karefilakis CM, Starakis IK, 2009. The Safety and Efficacy of the Fruit Juice of Ecballium elaterium in the Treatment of Acute Rhinosinusitis. Journal of Alternative and Complementary Medicine, 15 (12): 1273-1274.
Molavi O, Torkzaban F, Jafari S, Asnnashari S, Asgharian P, 2020. Chemical Compositions and Anti-Proliferative Activity of the Aerial Parts and Rhizomes of Squirting Cucumber, Cucurbitaceae. Jundishapur Journal of Natural Pharmaceutical Products, 15 (1): e82990.
Mukherjee AK, Basu S, Sarkar N, Ghosh AC, 2001. Advances in Cancer Therapy with Plant Based Natural Products. Current Medicinal Chemistry, 8 (12): 1467-1486.
Raikhlin-Eisenkraft B, Bentur Y, 2000. Ecballium elaterium (Squirting Cucumber) — Remedy or Poison? Journal of Toxicology. Clinical Toxicology, 38: 305-308.
Rao MM, Meshulam H, Lavie D, 1974. The Constituents of Ecballium elaterium L. Part XXIII Cucurbitacins and Hexanorcucurbitacins. Journal of the Chemical Society, Perkin Transactions, 1: 2552-2556.
Touihri-Barakati I, Kallech-Ziri O, Boulila A, Khwaldia K, Marrakchi N, Hanchi B, Hosni K, Luis J, 2016. Targetting v3 and 51 Integrins with Ecballium elaterium (L.) A. Rich. Seed Oil. Biomedicine and Pharmacotherapy, 84: 1223-1232.
Touihri-Barakati I, Kallech-Ziri O, Ayadi W, Kovaciç H, Hanchi B, Hüsnü K, Luis J, 2017. Cucurbitacin B Purified from Ecballium elaterium (L.) A. Rich from Tunisia Inhibits 51 Integrin-Mediated Adhesion, Migration, Proliferation of Human Glioblastoma Cell Line and Angiogenesis. European Journal of Pharmacology, 797: 53-161.
Touihri I, Kallech-Ziri O, Boulila A, Fatnassi S, Marrakchi N, Luis J, Hanchi B, 2019. Ecballium elaterium (L.) A. Rich. Seed Oil: Chemical Composition and Antiproliferative Effect on Human Colonic Adenocarcinoma and Fibrosarcoma Cancer Cell Lines. Arabian Journal of Chemistry, 12: 2347-2355.
Türkiye İstatistik Kurumu (TİK), 2020. Ölüm Nedeni İstatistikleri 2018. http://www.tuik.gov.tr/PreTabloArama.do?metod=search&araType=hb_x (Erişim tarihi: 07.07.2020).
Yılmaz K, Karakuş F, Eyol E, Tosun E, Yılmaz İ, Ünüvar S, 2018. Cytotoxic Effects of Cucurbitacin I and Ecballium elaterium on Breast Cancer Cells. Natural Product Communications, 13: 1445-1448.