Deneysel Florozis Oluştulmuş Ratlarda Kitosan ve Kitosan Oligosakkaridin Serum ve Doku Siyalik Asit Düzeyleri Üzerindeki Etkileri

Bu çalışmada deneysel florozisli ratlarda, kitosan (CH) ve kitosan oligosakkaritin (COS), serum ve dokularda (karaciğer, böbrek, beyin ve testis) toplam siyalik asit (TSA) düzeyine etkisi araştırılmıştır. Gruplar kontrol, sodyum florür (NaF), NaF + kitosan (NaF + CH), NaF + kitosan oligosakkarit (NaF + COS), kitosan (CH) ve kitosan oligosakkarit (COS) olarak oluşturuldu. NaF gruplarının içme suyu 100 ppm sodyum florür konsantrasyonunda hazırlandı. Deney gruplarında, doksan gün süreyle kitosan ve kitosan oligosakkarit 250 mg/kg dozunda oral yolla uygulandı. Çalışma sonunda serum ve karaciğer, böbrek, beyin ve testis doku homojenizatlarının TSA düzeyi spektrofotometrik yöntemle belirlendi. Florür uygulanan grupta (NaF), kontrol grubu ile karşılaştırıldığında, serum, karaciğer, böbrek, beyin ve testis dokularında TSA düzeylerinin arttığı görüldü (p<0.05). NaF grubuna göre, NaF+CH grubunda serum seviyelerinde, NaF+COS grubunda ise serum, karaciğer ve böbrek dokularında belirgin düşüş vardı. Beyin dokusu sialik asit düzeyi açısından kontrol ve deney grupları arasında fark olmadığı belirlendi (p>0.05). Sonuç olarak, flor toksikasyonunun, serum ve dokularda hücre hasarına neden olarak, TSA düzeylerinde artışa neden olduğu düşünülebilir. Sunulan çalışmada, CH ve COS'un TSA seviyelerini düşürdüğü gösterilmiştir. Ayrıca bu çalışmada, COS'un TSA seviyesini azaltmada daha etkili olduğu görüldü.

Anahtar Kelimeler:

Florozis, Sodyum florid, Total siyalik asit, Serum, Doku

Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis

In this study, the effect of chitosan (CH) and chitosan oligosaccharide (COS) on serum and tissue (liver, kidney, brain and testis) total sialic acid (TSA) level was investigated in rats with experimental fluorosis. The groups were formed as control, sodium fluoride (NaF), NaF+chitosan (NaF+CH), NaF+chitosan oligosaccharide (NaF+COS), chitosan (CH) and chitosan oligosaccharide (COS). Drinking water of NaF groups was prepared at a concentration of 100 ppm sodium fluoride. Chitosan and chitosan oligosaccharide were given to Experimental groups as 250 mg/kg dose by gastric gavage for ninety days. At the end of the study, TSA level was determined in serum, liver, kidney, brain and testicular tissues. Compared with the control group, it was found that TSA levels increased in serum, liver, kidney, brain and testis tissues in the group treated with sodium fluoride (p<0.05). According to the NaF group, there was a significant decrease in serum levels in the NaF+CH group and in the serum, liver and kidney tissues in the NaF+COS group. It was determined that there was no difference between the control and experimental groups in terms of brain tissue sialic acid level (p>0.05). In conclusion, it can be thought that fluorine intoxication causes an increase in TSA levels by causing cell damage in serum and tissues. In the study presented, CH and COS have been shown to reduce TSA levels. Also, in this study, COS was found to be more effective in reducing the TSA level.

Keywords:

Fluorosis, Sodium fluoride, Total sialic acid, Serum, Tissue,

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